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  4. Ubiquitin Antibody (YA022)

Ubiquitin Antibody (YA022)

Cat. No.: HY-P80363
Data Sheet SDS COA User Guide for Antibodies Technical Support

Ubiquitin Antibody (YA022) is a non-conjugated and Rabbit origined monoclonal antibody , targeting to Ubiquitin. It can be used for WB,ICC/IF,IHC-P,FC assays with tag free, in the background of Human, Mouse.

For research use only. We do not sell to patients.

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10 μL In-stock
50 μL In-stock
100 μL In-stock
250 μL   Get quote  

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Description

Ubiquitin Antibody (YA022) is a non-conjugated and Rabbit origined monoclonal antibody , targeting to Ubiquitin. It can be used for WB,ICC/IF,IHC-P,FC assays with tag free, in the background of Human, Mouse.

Tag

Free

Gene ID
SwissProt ID
Molecular Weight

Observed band size: 8-300 kDa

Purity

Protein A affinity purified.

Subcellular Location

Cytoplasm, Nucleus, Membrane, Mitochondrion.

Conjugation

Non-conjugated

Modification

Unmodified

RRID
Research Field

Neuroscience

Product Categories

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Clonality

Recombinant, Monoclonal

Host

Rabbit

Reactivity

Human, Mouse, Rat

Dilution Ratio

WB: 1:500 ;ICC/IF: 1:50-1:200 ;IHC-P: 1:200-1:500 ;FC: 1:50-1:100

  • Immunohistochemical analysis of paraffin-embedded Mouse esophagus tissue using Ubiquitin Antibody (YA022). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80363, 1/1000) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Immunohistochemical analysis of paraffin-embedded Mouse esophagus tissue using Ubiquitin Antibody (YA022). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80363, 1/1000) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Application

WB, ICC/IF, IHC-P, FC

Appearance

Liquid

Formulation

Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Isotype

IgG

Sensitivity

Endogenous

Immunogen

Synthetic peptide corresponding to Human Ubiquitin.AA range:90-120.

Database
Documentation

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Product Name:
Ubiquitin Antibody (YA022)
Cat. No.:
HY-P80363
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