2. Antibodies
  3. Primary Antibodies
  4. Polyclonal Antibodies Flow Cytometry Antibodies
  5. Ubiquitin Antibody

Ubiquitin Antibody is a non-conjugated and Rabbit origined polyclonal antibody, targeting to Ubiquitin. It can be used for WB,IHC-P,ICC/IF,FC assays with tag free, in the background of Human, Mouse, Rat.

For research use only. We do not sell to patients.

Size Price Stock Quantity
10 μL USD 70 In-stock
50 μL USD 185 In-stock
100 μL USD 300 In-stock
250 μL   Get quote  

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Description

Ubiquitin Antibody is a non-conjugated and Rabbit origined polyclonal antibody, targeting to Ubiquitin. It can be used for WB,IHC-P,ICC/IF,FC assays with tag free, in the background of Human, Mouse, Rat.
Background

Ubiquitin: This gene encodes ubiquitin, one of the most conserved proteins known. Ubiquitin has a major role in targeting cellular proteins for degradation by the 26S proteosome. It is also involved in the maintenance of chromatin structure, the regulation of gene expression, and the stress response. Ubiquitin is synthesized as a precursor protein consisting of either polyubiquitin chains or a single ubiquitin moiety fused to an unrelated protein. This gene consists of three direct repeats of the ubiquitin coding sequence with no spacer sequence. Consequently, the protein is expressed as a polyubiquitin precursor with a final amino acid after the last repeat. An aberrant form of this protein has been detected in patients with Alzheimer's disease and Down syndrome. Pseudogenes of this gene are located on chromosomes 1, 2, 13, and 17. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013]
Tag

Free
Gene ID
SwissProt ID
Synonyms
FLJ25987; MGC8385; ubiquitin B; Ubiquitin; UBCEP1; UBCEP2; RPS27A
Molecular Weight

Predicted band size: 8 kDa; Observed band size: 8-300 kDa
Purity

affinity purified
Conjugation

Non-conjugated
Modification

Unmodified
RRID
Research Field

Neuroscience
Product Categories

Primary Antibody; Rabbit Polyclonal Antibody
Clonality

Polyclonal
Host

Rabbit
Reactivity

Human, Mouse, Rat
Dilution Ratio

WB: 1:500-1:1,000 ;IHC: 1:50-1:100 ;IF: 1:50-1:200 ;FC: 1:50-1:100
Application

WB, IHC-P, ICC/IF, FC
Appearance

Liquid
Formulation

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.
Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Shipping

Shipping with blue ice.
Isotype

IgG
Sensitivity

Endogenous
Immunogen

Synthetic peptide corresponding to Human Ubiquitin.The exact sequence is proprietary to MCE.
Database

Entrez Gene: 7314 Human ; 22187 Mouse ; 192255 Rat

SwissProt: P0CG47 Human ; P0CG48 Human ; P62979 Human ; P62987 Human ; P62988 Human ; P0CG49 Mouse ; P62991 Mouse ; P0CG51 Rat P62989

OMIM: 191339 Human

Documentation
Data Sheet (235 KB) SDS (251 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)

Ubiquitin Antibody Related Classifications

Help & FAQs
  • How should lyophilized recombinant proteins be reconstituted and stored?

    1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss. 2. Taking 10 μg as an example, first add 20 μL of reconstituted solution provided by MCE and use a pipette to gently resuspend the lyophilized protein until it is fully dissolved.. (For most proteins, the reconstitution solution we provide is sterile water. If a diluent other than water is required, it will be indicated in the product's Certificate of Analysis (COA).). 3. Add an additional 80 μL of buffer/culture medium containing carrier protein (either 0.1% BSA, 5% HSA, 10% FBS, or 5% trehalose), and then use a pipette to gently mix until uniform. The final concentration is should not be lower than 100 μg/mL. 4. Aliquot at least 20 μL per tube. 5. After aliquoting, store it frozen at a temperature ranging from -20ºC to -80ºC, and it can be preserved for 3 to 6 months.

  • How should solution-form recombinant proteins be stored?

    1. The product can be stored in its original form and diluted as needed upon use. 2. Alternatively, dilute with a buffer/culture medium containing a carrier protein (either 0.1% BSA, 5% HSA, 10% FBS, or 5% alginate), mix well by pipetting, and ensure that

  • Why is it necessary to add carrier proteins?

    Carrier proteins are commonly added to enhance the stability of recombinant proteins, preventing them from adhering to the walls of the container during freezing or thawing processes. Plastic tubes have a certain adsorptive capacity for proteins, which may lead to difficulty in separating the protein from the tube walls, resulting in a decrease in the actual concentration of the protein in the solution and thus affecting its activity. To minimize such losses, it is recommended to add a commonly used carrier protein solution prior to the long-term storage of recombinant protein products.

  • Carrier protein types and options?

    In cases where the carrier protein is not expected to influence the experimental outcomes, an appropriate carrier protein, such as 0.1% BSA (Bovine Serum Albumin), 5% HSA (Human Serum Albumin), 10% FBS (Fetal Bovine Serum), or 5% trehalose, can be incorpo

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