Antimicrobial agent-8

Name: Antimicrobial agent-8
Brand: MedChemExpress
SKU: HY-151402
Rating: 4.5 (1 reviews)

Cat. No.: HY-151402 Purity: 95.12%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Antimicrobial agent-8 (Compound 15) is a potent antimicrobial agent, and shows potent antimicrobial activity with an MIC range of 2-8 μg/mL against Gram-negative and Gram-positive bacteria. Antimicrobial agent-8 shows anti-inflammatory activity against lipopolysaccharide-induced inflammation.

For research use only. We do not sell to patients.

Antimicrobial agent-8 Chemical Structure

CAS No. : 2978694-22-1

Size	Price	Stock	Quantity
5 mg	USD 625	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 1000	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 2000	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 3200	In-stock	Estimated Time of Arrival: December 31
100 mg		Get quote
200 mg		Get quote

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Based on 1 publication(s) in Google Scholar

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Biological Activity
Purity & Documentation
References
Customer Review

Description

In Vitro

Antimicrobial agent-8 (2.8-56.4 μM; 24 h) inhibits Gram-negative bacteria and Gram-positive bacteria growth^[1].
Antimicrobial agent-8 (5 and 20 μg/mL; 18 h) inhibits the production of nitric oxide (NO) and tumor necrosis factor-α (TNF-α) by lipopolysaccharide-stimulated in RAW 264.7 cells^[1].
Antimicrobial agent-8 (1-32 μg/mL, 16 h; 8-128 μg/mL; 24 h) shows potent biofilm inhibitory (MBIC₅₀=1 μg/mL) and eradicating activities (MBEC₅₀=8 μg/mL) by MDRPA bacteria^[1].
Antimicrobial agent-8 exhibits proteolytic resistance and salt/serum stability^[1].
Antimicrobial agent-8 displays synergistic or additive effects when combined with selected clinically used antibiotics^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:	E. coli [KCTC 1682], P. aeruginosa [KCTC 1637], S. epidermidis [KCTC 1917] and S. aureus [KCTC1621]
Concentration:	2.8-56.4 μM
Incubation Time:	24 hours
Result:	Inhibited Gram-negative bacteria with MIC values of 5.4 μM for E. coli [KCTC 1682] and P. aeruginosa [KCTC 1637]. Inhibited Gram- positive bacteria with MIC values of 2.7 μM and 5.4 μM for S. epidermidis [KCTC 1917] and S. aureus [KCTC1621], respectively.

Cell Viability Assay^[1]

Cell Line:	RAW 264.7 macrophages
Concentration:	5 and 20 μg/mL
Incubation Time:	18 hours
Result:	Observed LPS-stimulated production of NO with an inhibitory rate of 90.79% at 5 μg/mL. Exhibited inhibitory effects on the LPS-stimulated production of TNF-α with an inhibitory rate of 95.4% at 20 μg/mL.

Molecular Weight

746.95

Formula

C₃₉H₅₄N₁₆

CAS No.

2978694-22-1

Appearance

Solid

Color

White to off-white

SMILES

NCCN(C1=NC(NCC2=CC=CC=C2)=NC(N3CCN(C4=NC(NCC5=CC=CC=C5)=NC(N(CCN)CCN)=N4)C(CC6=CC=CC=C6)C3)=N1)CCN

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (133.88 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.3388 mL	6.6939 mL	13.3878 mL
5 mM	0.2678 mL	1.3388 mL	2.6776 mL
10 mM	0.1339 mL	0.6694 mL	1.3388 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: 2.5 mg/mL (3.35 mM); Clear solution; Need ultrasonic

This protocol yields a clear solution of 2.5 mg/mL.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 2.5 mg/mL (3.35 mM); Clear solution; Need ultrasonic

This protocol yields a clear solution of 2.5 mg/mL.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: 2.5 mg/mL (3.35 mM); Clear solution; Need ultrasonic

This protocol yields a clear solution of 2.5 mg/mL. If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 95.12%

Select Batch:

Data Sheet (275 KB) SDS (251 KB)

COA (251 KB) HNMR (159 KB) RP-HPLC (168 KB) LCMS (261 KB)

Handling Instructions (2659 KB)

References

[1]. Dinesh Kumar S, et al. Cationic, amphipathic small molecules based on a triazine-piperazine-triazine scaffold as a new class of antimicrobial agents. Eur J Med Chem. 2022 Sep 8;243:114747. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	1.3388 mL	6.6939 mL	13.3878 mL	33.4694 mL
	5 mM	0.2678 mL	1.3388 mL	2.6776 mL	6.6939 mL
	10 mM	0.1339 mL	0.6694 mL	1.3388 mL	3.3469 mL
	15 mM	0.0893 mL	0.4463 mL	0.8925 mL	2.2313 mL
	20 mM	0.0669 mL	0.3347 mL	0.6694 mL	1.6735 mL
	25 mM	0.0536 mL	0.2678 mL	0.5355 mL	1.3388 mL
	30 mM	0.0446 mL	0.2231 mL	0.4463 mL	1.1156 mL
	40 mM	0.0335 mL	0.1673 mL	0.3347 mL	0.8367 mL
	50 mM	0.0268 mL	0.1339 mL	0.2678 mL	0.6694 mL
	60 mM	0.0223 mL	0.1116 mL	0.2231 mL	0.5578 mL
	80 mM	0.0167 mL	0.0837 mL	0.1673 mL	0.4184 mL
	100 mM	0.0134 mL	0.0669 mL	0.1339 mL	0.3347 mL