2. Cell Cycle/DNA Damage
  3. Eukaryotic Initiation Factor (eIF)
  4. ATF4-IN-1

ATF4-IN-1 (Compound 21) is an ATF4 inhibitor and also an eIF2B activator. ATF4-IN-1 can be used in research on neurodegenerative diseases.

For research use only. We do not sell to patients.

ATF4-IN-1 Chemical Structure

ATF4-IN-1 Chemical Structure

CAS No. : 2991057-76-0

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5 mg USD 610 In-stock
10 mg USD 980 In-stock
25 mg USD 1980 In-stock
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Customer Review

Based on 1 publication(s) in Google Scholar

ATF4-IN-1 Related Antibodies

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  • Biological Activity

  • Purity & Documentation

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Description

ATF4-IN-1 (Compound 21) is an ATF4 inhibitor and also an eIF2B activator. ATF4-IN-1 can be used in research on neurodegenerative diseases[1].

In Vitro

ATF4-IN-1 dose-dependently inhibits the expression of ATF4 in HEK-293T cells, with an IC50 of 32.43 nM[1].
ATF4-IN-1 (0-1000 nM, 3 h) reduces ATF4 protein expression in HEK-293T cells[1].
ATF4-IN-1 (200 nM, 3 h) inhibits the transcription of ATF4 mRNA in HeLa cells[1].
ATF4-IN-1 activates eIF2B, with an EC50 of 5.844 nM in HEK-293T cells[1].
ATF4-IN-1 has an IC50 of 96 μM for inhibiting the proliferation of HEK-293T cells.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

ATF4-IN-1 Related Antibodies

Western Blot Analysis[1]

Cell Line: HEK-293 T
Concentration: 0, 1, 10, 100, 500, 1000 nM
Incubation Time: 3 h
Result: Inhibited ATF4 expression.

Real Time qPCR[1]

Cell Line: HeLa
Concentration: 200 nM
Incubation Time: 3 h
Result: Inhibited mRNA transcription of ATF4.
Molecular Weight

486.35

Formula

C24H21Cl2N3O4
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O=C(N[C@H]1CC[C@H](C2=NN=C(C3=CC4=CC(Cl)=CC=C4O3)O2)CC1)COC5=CC=C(Cl)C=C5
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 8.33 mg/mL (17.13 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.0561 mL 10.2807 mL 20.5613 mL
5 mM 0.4112 mL 2.0561 mL 4.1123 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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  • Dilution Calculator

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This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo Dissolution Calculator
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.0561 mL 10.2807 mL 20.5613 mL 51.4033 mL
5 mM 0.4112 mL 2.0561 mL 4.1123 mL 10.2807 mL
10 mM 0.2056 mL 1.0281 mL 2.0561 mL 5.1403 mL
15 mM 0.1371 mL 0.6854 mL 1.3708 mL 3.4269 mL
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ATF4-IN-1 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

ATF4-IN-12991057-76-0Eukaryotic Initiation Factor (eIF)amyotrophic lateral sclerosisALSADeIF2BInhibitorinhibitorinhibit

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