Ayanin

Name: Ayanin
Brand: MedChemExpress
SKU: HY-N2913
Rating: 4.5 (1 reviews)

Cat. No.: HY-N2913 Purity: 98.18%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Ayanin is a bioflavonoid isolated from Psychotria serpens. Ayanin is a non-selective phosphodiesterase_1-4 inhibitor and can be used for the study of respiratory disease,such as allergic asthma et al.

For research use only. We do not sell to patients.

Ayanin Chemical Structure

CAS No. : 572-32-7

Size	Price	Stock	Quantity
1 mg	USD 210	In-stock	Estimated Time of Arrival: December 31
5 mg	USD 440	In-stock	Estimated Time of Arrival: December 31
10 mg		Get quote
50 mg		Get quote

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This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 1 publication(s) in Google Scholar

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PDE1 PDE2 PDE3 PDE4 PDE5 PDE6 PDE7 PDE9 PDE10 PDE11 PDE12 PDE8 Autotaxin

Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target^[1]

PDE2A

PDE4

Cellular Effect

Cell Line	Type	Value	Description	References
2008	IC₅₀	50 μM Compound: 16	Inhibition of MRP1 expressed in human 2008 cells assessed as calcein AM accumulation by cell based assay Inhibition of MRP1 expressed in human 2008 cells assessed as calcein AM accumulation by cell based assay	[PMID: 21354800]
A2780 ADR	IC₅₀	9.4 μM Compound: 16	Inhibition of P-gp expressed in A2780adr cells by calcein AM accumulation assay Inhibition of P-gp expressed in A2780adr cells by calcein AM accumulation assay	[PMID: 21354800]
A549	IC₅₀	20.79 μM Compound: 6a	Anticancer activity against human A549 cells by HTS assay Anticancer activity against human A549 cells by HTS assay	[PMID: 25139569]
Calu-1	IC₅₀	> 50 μM Compound: 6a	Anticancer activity against human Calu1 cells by HTS assay Anticancer activity against human Calu1 cells by HTS assay	[PMID: 25139569]
HeLa	IC₅₀	19.47 μM Compound: 6a	Anticancer activity against human HeLa cells by HTS assay Anticancer activity against human HeLa cells by HTS assay	[PMID: 25139569]
HOP-62	IC₅₀	20.06 μM Compound: 6a	Anticancer activity against human HOP62 cells by HTS assay Anticancer activity against human HOP62 cells by HTS assay	[PMID: 25139569]
LOX IMVI	IC₅₀	45.97 μM Compound: 6a	Anticancer activity against human LOXIMVI cells by HTS assay Anticancer activity against human LOXIMVI cells by HTS assay	[PMID: 25139569]
M14	IC₅₀	37.07 μM Compound: 6a	Anticancer activity against human M14 cells by HTS assay Anticancer activity against human M14 cells by HTS assay	[PMID: 25139569]
MCF7	IC₅₀	0.68 μM Compound: 16	Inhibition of BCRP expressed in MCF-7 MX cells using Hoechst 33342 staining Inhibition of BCRP expressed in MCF-7 MX cells using Hoechst 33342 staining	[PMID: 21354800]
MDCK	IC₅₀	0.46 μM Compound: 16	Inhibition of BCRP expressed in MDCK cells using Hoechst 33342 staining Inhibition of BCRP expressed in MDCK cells using Hoechst 33342 staining	[PMID: 21354800]
MDCK	IC₅₀	13 μM Compound: 16	Inhibition of MDR1 expressed in MDCK cells using rhodamine 123 staining by flow cytometry Inhibition of MDR1 expressed in MDCK cells using rhodamine 123 staining by flow cytometry	[PMID: 21354800]
NCI-H1299	IC₅₀	> 50 μM Compound: 6a	Anticancer activity against human H1299 cells by HTS assay Anticancer activity against human H1299 cells by HTS assay	[PMID: 25139569]
NCI-H157	IC₅₀	18.64 μM Compound: 6a	Anticancer activity against human NCI-H157 cells by HTS assay Anticancer activity against human NCI-H157 cells by HTS assay	[PMID: 25139569]
NCI-H1792	IC₅₀	> 50 μM Compound: 6a	Anticancer activity against human NCI-H1792 cells by HTS assay Anticancer activity against human NCI-H1792 cells by HTS assay	[PMID: 25139569]
NCI-H1944	IC₅₀	21.92 μM Compound: 6a	Anticancer activity against human NCI-H1944 cells by HTS assay Anticancer activity against human NCI-H1944 cells by HTS assay	[PMID: 25139569]
NCI-H460	IC₅₀	12.79 μM Compound: 6a	Anticancer activity against human H460 cells by HTS assay Anticancer activity against human H460 cells by HTS assay	[PMID: 25139569]
NCI-H460	IC₅₀	5.7 μM Compound: 9	Inhibition of ABCG2 expressed in human NCI-H460 cells assessed as inhibition of PhA accumulation after 2 to 20 hrs relative to fumitremorgin C Inhibition of ABCG2 expressed in human NCI-H460 cells assessed as inhibition of PhA accumulation after 2 to 20 hrs relative to fumitremorgin C	[PMID: 21275386]
NCI-H522	IC₅₀	20.21 μM Compound: 6a	Anticancer activity against human NCI-H522 cells by HTS assay Anticancer activity against human NCI-H522 cells by HTS assay	[PMID: 25139569]

In Vitro

Ayanin inhibits interleukin (IL)-4 production from purified basophils with an IC₅₀ value of 2.2 μM^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

344.32

Formula

C₁₈H₁₆O₇

CAS No.

572-32-7

Appearance

Solid

Color

White to yellow

SMILES

O=C1C(OC)=C(C2=CC=C(OC)C(O)=C2)OC3=CC(OC)=CC(O)=C13

Structure Classification

Others

Initial Source

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility

In Vitro:

DMSO : 250 mg/mL (726.07 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.9043 mL	14.5214 mL	29.0428 mL
5 mM	0.5809 mL	2.9043 mL	5.8085 mL
10 mM	0.2904 mL	1.4521 mL	2.9043 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (6.04 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 98.18%

Select Batch:

Data Sheet (273 KB) SDS (251 KB)

COA (249 KB) LCMS (90 KB)

Handling Instructions (2659 KB)

References

[1]. Fei-Peng Lee, et al. Ayanin, a Non-Selective Phosphodiesterase 1-4 Inhibitor, Effectively Suppresses Ovalbumin-Induced Airway Hyperresponsiveness Without Affecting xylazine/ketamine-induced Anesthesia. Eur J Pharmacol. 2010 Jun 10;635(1-3):198-203. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.9043 mL	14.5214 mL	29.0428 mL	72.6069 mL
	5 mM	0.5809 mL	2.9043 mL	5.8086 mL	14.5214 mL
	10 mM	0.2904 mL	1.4521 mL	2.9043 mL	7.2607 mL
	15 mM	0.1936 mL	0.9681 mL	1.9362 mL	4.8405 mL
	20 mM	0.1452 mL	0.7261 mL	1.4521 mL	3.6303 mL
	25 mM	0.1162 mL	0.5809 mL	1.1617 mL	2.9043 mL
	30 mM	0.0968 mL	0.4840 mL	0.9681 mL	2.4202 mL
	40 mM	0.0726 mL	0.3630 mL	0.7261 mL	1.8152 mL
	50 mM	0.0581 mL	0.2904 mL	0.5809 mL	1.4521 mL
	60 mM	0.0484 mL	0.2420 mL	0.4840 mL	1.2101 mL
	80 mM	0.0363 mL	0.1815 mL	0.3630 mL	0.9076 mL
	100 mM	0.0290 mL	0.1452 mL	0.2904 mL	0.7261 mL

Ayanin Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Ayanin572-32-7Phosphodiesterase (PDE)respiratory diseasetracheal relaxanthypertensionairway hyperresponsivenessallergic asthmaInhibitorinhibitorinhibit

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Ayanin

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Ayanin Related Antibodies

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References

Customer Review

Ayanin Related Antibodies

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Complete Stock Solution Preparation Table

Ayanin Related Classifications

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