2. Anti-infection Cell Cycle/DNA Damage Metabolic Enzyme/Protease Immunology/Inflammation NF-κB Apoptosis
  3. HIV DNA/RNA Synthesis HBV Reactive Oxygen Species Apoptosis
  4. AZT triphosphate tetraammonium

AZT triphosphate tetraammonium  (Synonyms: 3'-Azido-3'-deoxythymidine-5'-triphosphate tetraammonium)

Cat. No.: HY-116364B Purity: 99.45%
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AZT triphosphate (3'-Azido-3'-deoxythymidine-5'-triphosphate) tetraammonium is an active triphosphate metabolite of Zidovudine (AZT). AZT triphosphate tetraammonium exhibits antiretroviral activity and inhibits replication of HIV. AZT triphosphate tetraammonium also inhibits the DNA polymerase of HBV. AZT triphosphate tetraammonium activates the mitochondria-mediated apoptosis pathway.

For research use only. We do not sell to patients.

AZT triphosphate tetraammonium Chemical Structure

AZT triphosphate tetraammonium Chemical Structure

CAS No. : 106060-92-8

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1 mg USD 250 In-stock
5 mg USD 950 In-stock
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25 mg USD 3200 In-stock
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100 mg USD 8200 In-stock
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Based on 1 publication(s) in Google Scholar

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AZT triphosphate tetraammonium Related Antibodies

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Description

AZT triphosphate (3'-Azido-3'-deoxythymidine-5'-triphosphate) tetraammonium is an active triphosphate metabolite of Zidovudine (AZT). AZT triphosphate tetraammonium exhibits antiretroviral activity and inhibits replication of HIV. AZT triphosphate tetraammonium also inhibits the DNA polymerase of HBV. AZT triphosphate tetraammonium activates the mitochondria-mediated apoptosis pathway[1][2][3].

Cellular Effect
Cell Line Type Value Description References
Sf9 IC50
22100 nM
Compound: AZT-TP
Inhibition of HCMV recombinant DNA polymerase expressed in baculovirus infected SF9 cells after 12 mins by scintillation proximity assay
Inhibition of HCMV recombinant DNA polymerase expressed in baculovirus infected SF9 cells after 12 mins by scintillation proximity assay
[PMID: 20167488]
Sf9 IC50
5800 nM
Compound: AZT-TP
Inhibition of Varicella zoster virus recombinant DNA polymerase expressed in baculovirus infected SF9 cells after 12 mins by scintillation proximity assay
Inhibition of Varicella zoster virus recombinant DNA polymerase expressed in baculovirus infected SF9 cells after 12 mins by scintillation proximity assay
[PMID: 20167488]
In Vitro

AZT triphosphate (3'-Azido-3'-deoxythymidine-5'-triphosphate) tetraammonium accumulation disrupts the mitochondrial tubular network in H9c2 cells through Treatment with 100 µM Zidovudine (AZT) for 48h. AZT triphosphate tetraammonium accumulation causes downregulation of Opa1 and upregulation of Drp1. AZT triphosphate tetraammonium causes mitochondrial dysfunction, increases the production of cytotoxic reactive oxygen species (ROS), and impairs the balance of the mitochondrial quality control system in H9c2 cell model established from rat embryonic myoblasts[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

AZT triphosphate tetraammonium Related Antibodies
Molecular Weight

575.30

Formula

C10H28N9O13P3
CAS No.
Appearance

Solid

Color

Off-white to light yellow

SMILES

O=C1NC(N([C@@H](C[C@@H]2N=[N+]=[N-])O[C@@H]2COP(OP(O)(OP(O)(O)=O)=O)(O)=O)C=C1C)=O.N.N.N.N
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

-20°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (173.82 mM; ultrasonic and warming and heat to 70°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.7382 mL 8.6911 mL 17.3822 mL
5 mM 0.3476 mL 1.7382 mL 3.4764 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    90% Corn Oil

    Solubility: ≥ 5 mg/mL (8.69 mM); Clear solution

    This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.7382 mL 8.6911 mL 17.3822 mL 43.4556 mL
5 mM 0.3476 mL 1.7382 mL 3.4764 mL 8.6911 mL
10 mM 0.1738 mL 0.8691 mL 1.7382 mL 4.3456 mL
15 mM 0.1159 mL 0.5794 mL 1.1588 mL 2.8970 mL
20 mM 0.0869 mL 0.4346 mL 0.8691 mL 2.1728 mL
25 mM 0.0695 mL 0.3476 mL 0.6953 mL 1.7382 mL
30 mM 0.0579 mL 0.2897 mL 0.5794 mL 1.4485 mL
40 mM 0.0435 mL 0.2173 mL 0.4346 mL 1.0864 mL
50 mM 0.0348 mL 0.1738 mL 0.3476 mL 0.8691 mL
60 mM 0.0290 mL 0.1449 mL 0.2897 mL 0.7243 mL
80 mM 0.0217 mL 0.1086 mL 0.2173 mL 0.5432 mL
100 mM 0.0174 mL 0.0869 mL 0.1738 mL 0.4346 mL
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AZT triphosphate tetraammonium Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

AZT triphosphate tetraammonium106060-92-83'-Azido-3'-deoxythymidine-5'-triphosphate tetraammoniumHIVDNA/RNA SynthesisHBVReactive Oxygen SpeciesApoptosisHuman immunodeficiency virusHepatitis B virusZidovudinetriphosphatemetaboliteROSmitochondrialcardiomyopathyOpa1Drp1cytotoxicityantiretroviralInhibitorinhibitorinhibit

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