Azvudine hydrochloride (Synonyms: RO-0622 hydrochloride; FNC hydrochloride)

Name: Azvudine hydrochloride
Brand: MedChemExpress
SKU: HY-19314A
Rating: 4.5 (1 reviews)

Cat. No.: HY-19314A Purity: 95.23%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Azvudine (RO-0622) hydrochloride is a potent nucleoside reverse transcriptase inhibitor (NRTI), with antiviral activity on HIV, HBV and HCV. Azvudine hydrochloride exerts highly potent inhibition on HIV-1 (EC₅₀s ranging from 0.03 to 6.92 nM) and HIV-2 (EC₅₀s ranging from 0.018 to 0.025 nM). Azvudine hydrochloride inhibits NRTI-resistant viral strains. Azvudine (hydrochloride) is a click chemistry reagent, it contains an Azide group and can undergo copper-catalyzed azide-alkyne cycloaddition reaction (CuAAc) with molecules containing Alkyne groups. It can also undergo strain-promoted alkyne-azide cycloaddition (SPAAC) reactions with molecules containing DBCO or BCN groups.

For research use only. We do not sell to patients.

Azvudine hydrochloride Chemical Structure

CAS No. : 1333126-31-0

Size	Price	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in Water ready for reconstitution	USD 187	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in Water	USD 187	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	USD 170	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 280	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 545	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 820	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 1200	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of Azvudine hydrochloride:

Azvudine Get quote

1 Publications Citing Use of MCE Azvudine hydrochloride

•Acta Pharm Sin B. 2024 Mar 30.

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HIV HIV-1 HIV-2

Biological Activity
Purity & Documentation
References
Customer Review

Description

Azvudine (RO-0622) hydrochloride is a potent nucleoside reverse transcriptase inhibitor (NRTI), with antiviral activity on HIV, HBV and HCV. Azvudine hydrochloride exerts highly potent inhibition on HIV-1 (EC₅₀s ranging from 0.03 to 6.92 nM) and HIV-2 (EC₅₀s ranging from 0.018 to 0.025 nM). Azvudine hydrochloride inhibits NRTI-resistant viral strains^[1]. Azvudine (hydrochloride) is a click chemistry reagent, it contains an Azide group and can undergo copper-catalyzed azide-alkyne cycloaddition reaction (CuAAc) with molecules containing Alkyne groups. It can also undergo strain-promoted alkyne-azide cycloaddition (SPAAC) reactions with molecules containing DBCO or BCN groups.

IC₅₀ & Target^[1]

HIV-1

0.03-6.92 nM (EC₅₀)

HIV-2

0.018-0.02 nM (EC₅₀)

Cellular Effect

Cell Line	Type	Value	Description	References
ACH-2 cell line	IC₅₀	0.11 μM Compound: 11	Cytotoxicity activity against human ACH2 by CellTiter-Glo luminescent cell viability assay Cytotoxicity activity against human ACH2 by CellTiter-Glo luminescent cell viability assay	[PMID: 21745701]
HEK-293T	IC₅₀	> 140 μM Compound: 11	Cytotoxicity activity against human 293T by CellTiter-Glo luminescent cell viability assay Cytotoxicity activity against human 293T by CellTiter-Glo luminescent cell viability assay	[PMID: 21745701]
HepG2	IC₅₀	> 140 μM Compound: 11	Cytotoxicity activity against human HepG2 by CellTiter-Glo luminescent cell viability assay Cytotoxicity activity against human HepG2 by CellTiter-Glo luminescent cell viability assay	[PMID: 21745701]
MT2	EC₅₀	0.13 nM Compound: 11	Antiviral activity against vesicular stomatitis virus G-pseudotyped Human immunodeficiency virus 1 NL4-3 infected in human MT2 cells assessed as inhibition of viral replication after 48 hrs by luciferase activity reporter gene assay Antiviral activity against vesicular stomatitis virus G-pseudotyped Human immunodeficiency virus 1 NL4-3 infected in human MT2 cells assessed as inhibition of viral replication after 48 hrs by luciferase activity reporter gene assay	[PMID: 21745701]
MT4	IC₅₀	0.12 μM Compound: 11	Cytotoxicity activity against human MT4 by CellTiter-Glo luminescent cell viability assay Cytotoxicity activity against human MT4 by CellTiter-Glo luminescent cell viability assay	[PMID: 21745701]
PBMC	IC₅₀	0.15 μM Compound: 11	Cytotoxicity activity against human PBMC by CellTiter-Glo luminescent cell viability assay Cytotoxicity activity against human PBMC by CellTiter-Glo luminescent cell viability assay	[PMID: 21745701]
TZM	IC₅₀	> 140 μM Compound: 11	Cytotoxicity activity against human TZM-bl by CellTiter-Glo luminescent cell viability assay Cytotoxicity activity against human TZM-bl by CellTiter-Glo luminescent cell viability assay	[PMID: 21745701]

In Vitro

Azvudine (RO-0622) hydrochloride displays strong inhibition on wild-type HIV-1_IIIB and HIV-1_RF with an EC₅₀ ranging from 30 to 110 pM. The EC₅₀ values of Azvudine hydrochloride against HIV-1_KM018, HIV-1_TC-1 and HIV-1_{WAN T69N} are 6.92, 0.34 and 0.45 nM, respectively. Azvudine is sensitive to NRTIs-resistant strain HIV-1_74V, PIs-resistant strains HIV-1_{L10R/M46I/L63P/V82T/I84V} and HIV-1_{RF V82F/184V}, and FIs-resistant strain pNL4-3_{gp41 (36G) V38A/N42T}. The EC₅₀ values of Azvudine hydrochloride against these resistant strains are 0.11, 0.14, 0.37 and 0.36 nM respectively^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Clinical Trial

Molecular Weight

322.68

Formula

C₉H₁₂ClFN₆O₄

CAS No.

1333126-31-0

Appearance

Solid

Color

Light yellow to khaki

SMILES

O[C@@H]1[C@](CO)(N=[N+]=[N-])O[C@@H](N2C(N=C(N)C=C2)=O)[C@H]1F.Cl

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

H₂O : 125 mg/mL (387.38 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.0990 mL	15.4952 mL	30.9905 mL
5 mM	0.6198 mL	3.0990 mL	6.1981 mL
10 mM	0.3099 mL	1.5495 mL	3.0990 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 14.29 mg/mL (44.29 mM); Clear solution; Need ultrasonic and warming and heat to 60°C

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 95.23%

Select Batch:

Data Sheet (276 KB) SDS (252 KB)

COA (250 KB) HNMR (255 KB) LCMS (95 KB)

Handling Instructions (2659 KB)

References

[1]. Wang RR, et al. Azvudine, a novel nucleoside reverse transcriptase inhibitor showed good drug combination features and better inhibition on drug-resistant strains than lamivudine in vitro. PLoS One. 2014 Aug 21;9(8):e105617. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O	1 mM	3.0990 mL	15.4952 mL	30.9905 mL	77.4761 mL
	5 mM	0.6198 mL	3.0990 mL	6.1981 mL	15.4952 mL
	10 mM	0.3099 mL	1.5495 mL	3.0990 mL	7.7476 mL
	15 mM	0.2066 mL	1.0330 mL	2.0660 mL	5.1651 mL
	20 mM	0.1550 mL	0.7748 mL	1.5495 mL	3.8738 mL
	25 mM	0.1240 mL	0.6198 mL	1.2396 mL	3.0990 mL
	30 mM	0.1033 mL	0.5165 mL	1.0330 mL	2.5825 mL
	40 mM	0.0775 mL	0.3874 mL	0.7748 mL	1.9369 mL
	50 mM	0.0620 mL	0.3099 mL	0.6198 mL	1.5495 mL
	60 mM	0.0517 mL	0.2583 mL	0.5165 mL	1.2913 mL
	80 mM	0.0387 mL	0.1937 mL	0.3874 mL	0.9685 mL
	100 mM	0.0310 mL	0.1550 mL	0.3099 mL	0.7748 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Azvudine hydrochloride Related Classifications

Infection

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Azvudine hydrochloride (Synonyms: RO-0622 hydrochloride; FNC hydrochloride)

Customer Review

Other Forms of Azvudine hydrochloride:

Azvudine hydrochloride Related Antibodies

1 Publications Citing Use of MCE Azvudine hydrochloride

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All HIV Isoform Specific Products:

Biological Activity

Purity & Documentation

References

Customer Review

Azvudine hydrochloride Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

Azvudine hydrochloride Related Classifications

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