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  4. Basic green 4

Basic green 4 (Malachite green) is a cationic dye that is widely used as a bactericide in aquaculture and is also commonly used in laboratories for the determination of phosphates. Basic green 4 exhibits high cytotoxicity and carcinogenicity to mammalian cells, specifically shown by its IC50 values of 2.03 µM for HEp-2 human laryngeal cells and 13.8 µM for Caco-2 human colorectal adenocarcinoma cells, and it can promote the development of liver tumors. The maximum absorbance wavelength is 621 nm.

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Basic green 4 Chemical Structure

Basic green 4 Chemical Structure

CAS No. : 569-64-2

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Based on 1 publication(s) in Google Scholar

Other Forms of Basic green 4:

Basic green 4 Related Antibodies

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1 Publications Citing Use of MCE Basic green 4

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  • Purity & Documentation

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Description

Basic green 4 (Malachite green) is a cationic dye that is widely used as a bactericide in aquaculture and is also commonly used in laboratories for the determination of phosphates. Basic green 4 exhibits high cytotoxicity and carcinogenicity to mammalian cells, specifically shown by its IC50 values of 2.03 µM for HEp-2 human laryngeal cells and 13.8 µM for Caco-2 human colorectal adenocarcinoma cells, and it can promote the development of liver tumors. The maximum absorbance wavelength is 621 nm[1][2][3].

In Vitro

Basic Green 4 Measurement of Phosphate[1]
(1) Preparation of Basic Green 4 storage solution: Slowly add concentrated sulfuric acid (60 mL, d=1.84 g/L) to 300 mL of water. Cool the solution to room temperature and add 0.44 g of Basic Green 4.
(2) Preparation of Basic Green 4 working solution: Add 10 mL of the dye to 2.5 mL of 7.5% ammonium molybdate, then add 0.2 mL of 11% Tween 20.
(3) Mix 1 volume of Basic Green 4 with 4 volumes of the phosphate sample.
(4) Let it sit for ten minutes, then measure the absorbance at 630 nm.
Basic Green 4 Fungal Inhibition[2]
(1) Collect fish eggs using spawning mats in a pond environment and rinse off the mud with well water.
(2) Place the fish eggs in a petri dish and add Basic Green 4 at a concentration of 2 ppm for 60 minutes, or at a concentration of 10 ppm for 10 minutes.
(3) Discard the Basic Green 4 and rinse the fish eggs multiple times with well water.
(4) Place the fish eggs in a petri dish with clean, oxygenated water and incubate at 22°C (72°F). Observe the newly hatched fry for 24 hours to determine the survival rate after treatment. Each concentration test is repeated six times.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Basic green 4 Related Antibodies
Molecular Weight

364.91

Formula

C23H25ClN2
CAS No.
Appearance

Solid

Color

Green to black

SMILES

C/[N+](C)=C1C=C/C(C=C/1)=C(C2=CC=C(N(C)C)C=C2)\C3=CC=CC=C3.[Cl-]
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (274.04 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H2O : 3.85 mg/mL (10.55 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.7404 mL 13.7020 mL 27.4040 mL
5 mM 0.5481 mL 2.7404 mL 5.4808 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation

COA (249 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O / DMSO 1 mM 2.7404 mL 13.7020 mL 27.4040 mL 68.5100 mL
5 mM 0.5481 mL 2.7404 mL 5.4808 mL 13.7020 mL
10 mM 0.2740 mL 1.3702 mL 2.7404 mL 6.8510 mL
DMSO 15 mM 0.1827 mL 0.9135 mL 1.8269 mL 4.5673 mL
20 mM 0.1370 mL 0.6851 mL 1.3702 mL 3.4255 mL
25 mM 0.1096 mL 0.5481 mL 1.0962 mL 2.7404 mL
30 mM 0.0913 mL 0.4567 mL 0.9135 mL 2.2837 mL
40 mM 0.0685 mL 0.3426 mL 0.6851 mL 1.7128 mL
50 mM 0.0548 mL 0.2740 mL 0.5481 mL 1.3702 mL
60 mM 0.0457 mL 0.2284 mL 0.4567 mL 1.1418 mL
80 mM 0.0343 mL 0.1713 mL 0.3426 mL 0.8564 mL
100 mM 0.0274 mL 0.1370 mL 0.2740 mL 0.6851 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Basic green 4 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Basic green 4569-64-2Malachite greenBasic green4Basic green-4Fluorescent Dyecationic dyeN-methylated diaminotriphenylmethane dyecoloring purposeInhibitorinhibitorinhibit

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