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  4. BETA-1

BETA-1 is the first twisted intramolecular charge transfer (TICT)-aggregation-induced emission (AIE) integration molecule. BETA-1 emits cyan fluorescence in lipid droplets (LDs) and red fluorescence in mitochondria. BETA-1 can be used for the simultaneous and dual-color imaging of LDs and mitochondria in vivo and in vitro.

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BETA-1 Chemical Structure

BETA-1 Chemical Structure

CAS No. : 2924598-24-1

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5 mg USD 185 In-stock
10 mg USD 300 In-stock
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Based on 1 publication(s) in Google Scholar

BETA-1 Related Antibodies

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Description

BETA-1 is the first twisted intramolecular charge transfer (TICT)-aggregation-induced emission (AIE) integration molecule. BETA-1 emits cyan fluorescence in lipid droplets (LDs) and red fluorescence in mitochondria. BETA-1 can be used for the simultaneous and dual-color imaging of LDs and mitochondria in vivo and in vitro[1].

In Vitro

BETA-1 can be used for imaging of LDs and Mitochondria in live cells[1].
Wavelength: cyan: λex = 405 nm, λem = 430–500 nm; red: λex = 561 nm, λem = 600–700 nm).
1. HeLa cells were cultured in DMEM supplemented with 10% FBS in a CO2/air (5%/95%) incubator at 37 °C.
2. A total of 5.0 × 103 HeLa cells per well were seeded on a glass-bottom 96-well plate and incubated for 24 h.
3. For cell imaging, live HeLa cells were stained with 5 μM BETA-1 at 37 °C for 30 min.
4. The emission of BETA-1 was collected under excitation at 405 or 561 nm. Digital images were recorded using an laser scanning confocal microscope. The images were analyzed with Fiji/ImageJ.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

BETA-1 Related Antibodies
Molecular Weight

705.67

Formula

C37H28IN3S2
CAS No.
Appearance

Solid

Color

Brown to black

SMILES

C[N+]1=C(/C=C/C2=CC=C(N(C3=CC=C(/C=C/C4=NC5=CC=CC=C5S4)C=C3)C6=CC=CC=C6)C=C2)SC7=CC=CC=C17.[I-]
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

DMSO : 25 mg/mL (35.43 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.4171 mL 7.0855 mL 14.1709 mL
5 mM 0.2834 mL 1.4171 mL 2.8342 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (3.54 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.4171 mL 7.0855 mL 14.1709 mL 35.4273 mL
5 mM 0.2834 mL 1.4171 mL 2.8342 mL 7.0855 mL
10 mM 0.1417 mL 0.7085 mL 1.4171 mL 3.5427 mL
15 mM 0.0945 mL 0.4724 mL 0.9447 mL 2.3618 mL
20 mM 0.0709 mL 0.3543 mL 0.7085 mL 1.7714 mL
25 mM 0.0567 mL 0.2834 mL 0.5668 mL 1.4171 mL
30 mM 0.0472 mL 0.2362 mL 0.4724 mL 1.1809 mL
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BETA-1 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

BETA-12924598-24-1BETA1BETA 1Fluorescent Dyeinter-organelle interactionslipid dropletsmitochondriaTICT-AIE mechanismcell imagingInhibitorinhibitorinhibit

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