Biotin-PEG4-NHS ester

Name: Biotin-PEG4-NHS ester
Brand: MedChemExpress
SKU: HY-140889
Rating: 4.5 (1 reviews)

Cat. No.: HY-140889 Purity: 99.56%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Biotin-PEG4-NHS ester is a biotin-labeled, PEG-based PROTAC linker that can be used in the synthesis of PROTACs.

For research use only. We do not sell to patients.

Biotin-PEG4-NHS ester Chemical Structure

CAS No. : 459426-22-3

Size	Price	Stock	Quantity
Free Sample (0.1 - 0.5 mg)		Apply Now
50 mg	USD 70	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 110	In-stock	Estimated Time of Arrival: December 31
250 mg	USD 200	In-stock	Estimated Time of Arrival: December 31
500 mg		Get quote
1 g		Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

1 Publications Citing Use of MCE Biotin-PEG4-NHS ester

•Pharmaceutics. 2023 Nov 23, 15(12), 2659.

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Biological Activity
Purity & Documentation
References
Customer Review

Description

Biotin-PEG4-NHS ester is a biotin-labeled, PEG-based PROTAC linker that can be used in the synthesis of PROTACs^[1].

IC₅₀ & Target

PEGs

In Vitro

PROTACs contain two different ligands connected by a linker; one is a ligand for an E3 ubiquitin ligase and the other is for the target protein. PROTACs exploit the intracellular ubiquitin-proteasome system to selectively degrade target proteins^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

588.67

Formula

C₂₅H₄₀N₄O₁₀S

CAS No.

459426-22-3

Appearance

Solid

Color

White to light yellow

SMILES

O=C(ON1C(CCC1=O)=O)CCOCCOCCOCCOCCNC(CCCC[C@@H]2SC[C@]([C@]2([H])N3)([H])NC3=O)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, sealed storage, away from moisture and light

*The compound is unstable in solutions, freshly prepared is recommended.

Solvent & Solubility

In Vitro:

DMSO : 125 mg/mL (212.34 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.6987 mL	8.4937 mL	16.9874 mL
5 mM	0.3397 mL	1.6987 mL	3.3975 mL
10 mM	0.1699 mL	0.8494 mL	1.6987 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (3.53 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (3.53 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.08 mg/mL (3.53 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*The compound is unstable in solutions, freshly prepared is recommended.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.56%

Select Batch:

Data Sheet (269 KB) SDS (393 KB)

COA (243 KB) HNMR (280 KB) RP-HPLC (215 KB) MS (68 KB)

Handling Instructions (2659 KB)

References

[1]. Gadd MS, et al. Structural basis of PROTAC cooperative recognition for selective protein degradation. Nat Chem Biol. 2017 May;13(5):514-521. [Content Brief]

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	1.6987 mL	8.4937 mL	16.9874 mL	42.4686 mL
	5 mM	0.3397 mL	1.6987 mL	3.3975 mL	8.4937 mL
	10 mM	0.1699 mL	0.8494 mL	1.6987 mL	4.2469 mL
	15 mM	0.1132 mL	0.5662 mL	1.1325 mL	2.8312 mL
	20 mM	0.0849 mL	0.4247 mL	0.8494 mL	2.1234 mL
	25 mM	0.0679 mL	0.3397 mL	0.6795 mL	1.6987 mL
	30 mM	0.0566 mL	0.2831 mL	0.5662 mL	1.4156 mL
	40 mM	0.0425 mL	0.2123 mL	0.4247 mL	1.0617 mL
	50 mM	0.0340 mL	0.1699 mL	0.3397 mL	0.8494 mL
	60 mM	0.0283 mL	0.1416 mL	0.2831 mL	0.7078 mL
	80 mM	0.0212 mL	0.1062 mL	0.2123 mL	0.5309 mL
	100 mM	0.0170 mL	0.0849 mL	0.1699 mL	0.4247 mL