2. Apoptosis
  3. Apoptosis
  4. BiP inducer X

BiP inducer X, a selective inducer of immunoglobulin heavy chain binding protein (BiP)/GRP78, is an effective ER (endoplasmic reticulum) stress inhibitor. BiP inducer X preferentially induces BiP with slight inductions of GRP94, calreticulin, and C/EBP homologous protein. BiP inducer X protects neurons from ER stress.

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BiP inducer X Chemical Structure

BiP inducer X Chemical Structure

CAS No. : 101714-41-4

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10 mM * 1 mL in DMSO
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Customer Review

Based on 6 publication(s) in Google Scholar

BiP inducer X Related Antibodies

Top Publications Citing Use of Products

    BiP inducer X purchased from MedChemExpress. Usage Cited in: Research Square Print. 2023 Mar 6.

    BiP inducer X (BIX; 25 mM) significantly reduces the expression levels of pro-apoptotic marker proteins including Bax, Bad, cytochrome-C, and caspase-1 in microglia cells.

    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    BiP inducer X, a selective inducer of immunoglobulin heavy chain binding protein (BiP)/GRP78, is an effective ER (endoplasmic reticulum) stress inhibitor. BiP inducer X preferentially induces BiP with slight inductions of GRP94, calreticulin, and C/EBP homologous protein. BiP inducer X protects neurons from ER stress[1][2].

    In Vitro

    BiP inducer X (5 μM; 0-12 hours; SK-N-SH cells) increase BiP protein[1].
    The induction of BiP by BiP inducer X (BIX) is mediated by activation of ER stress response elements (ERSEs) through the ATF6 pathway[1].
    BiP inducer X (5 μM; 12 hours; pretreatment with SK-N-SH cells) inhibits cell death induced by ER stress involving inhibited activation of caspases 3/7 and 4[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    BiP inducer X Related Antibodies
    In Vivo

    BiP inducer X administration reduces the insults due to cerebral infarction[1].
    A 20 μg portion of BiP inducer X administration reduces ER stress-induced apoptosis induced in the penumbra by MCA occlusion[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Male adult ddY mice weighing 24-34 g (Japan SLC)[1]
    Dosage: 20 μg (2 μl)
    Administration: Intracerebroventricularly
    Result: Significantly increased the level of BiP protein 24 h after administration, confirming that administration of BIX induces BiP protein in vivo.
    Molecular Weight

    209.22

    Formula

    C9H7NO3S
    CAS No.
    Appearance

    Solid

    Color

    Brown to gray

    SMILES

    N#CSCC(C1=CC(O)=C(O)C=C1)=O
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 6 months
    -20°C 1 month
    Solvent & Solubility
    In Vitro: 

    DMSO : 50 mg/mL (238.98 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 4.7797 mL 23.8983 mL 47.7966 mL
    5 mM 0.9559 mL 4.7797 mL 9.5593 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

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    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2.5 mg/mL (11.95 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

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    (per animal)

    g

    Dosing volume
    (per animal)

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    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
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    +
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    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    Purity & Documentation

    Purity: 99.88%

    COA (244 KB) HNMR (251 KB) LCMS (277 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 4.7797 mL 23.8983 mL 47.7966 mL 119.4914 mL
    5 mM 0.9559 mL 4.7797 mL 9.5593 mL 23.8983 mL
    10 mM 0.4780 mL 2.3898 mL 4.7797 mL 11.9491 mL
    15 mM 0.3186 mL 1.5932 mL 3.1864 mL 7.9661 mL
    20 mM 0.2390 mL 1.1949 mL 2.3898 mL 5.9746 mL
    25 mM 0.1912 mL 0.9559 mL 1.9119 mL 4.7797 mL
    30 mM 0.1593 mL 0.7966 mL 1.5932 mL 3.9830 mL
    40 mM 0.1195 mL 0.5975 mL 1.1949 mL 2.9873 mL
    50 mM 0.0956 mL 0.4780 mL 0.9559 mL 2.3898 mL
    60 mM 0.0797 mL 0.3983 mL 0.7966 mL 1.9915 mL
    80 mM 0.0597 mL 0.2987 mL 0.5975 mL 1.4936 mL
    100 mM 0.0478 mL 0.2390 mL 0.4780 mL 1.1949 mL
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    BiP inducer X Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    BiP inducer X101714-41-4ApoptosisGRP78BiPGRP94calreticulinneuronsERstressInhibitorinhibitorinhibit

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