Boc-Gly-Gly-Phe-Gly-OH

Name: Boc-Gly-Gly-Phe-Gly-OH
Brand: MedChemExpress
SKU: HY-P1449
Rating: 4.5 (1 reviews)

Cat. No.: HY-P1449 Purity: 99.41%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Boc-Gly-Gly-Phe-Gly-OH, a self-assembly of N- and C-protected tetrapeptide, is a protease cleavable linker used for the antibody-drug conjugate (ADC).

For research use only. We do not sell to patients.

Custom Peptide Synthesis

Boc-Gly-Gly-Phe-Gly-OH Chemical Structure

CAS No. : 187794-49-6

Size	Price	Stock	Quantity
100 mg	USD 25	In-stock	Estimated Time of Arrival: December 31
250 mg	USD 55	In-stock	Estimated Time of Arrival: December 31
1 g	USD 142	In-stock	Estimated Time of Arrival: December 31
5 g	USD 413	In-stock	Estimated Time of Arrival: December 31
10 g	USD 538	Get quote	Estimated Time of Arrival: December 31
50 g		Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of Boc-Gly-Gly-Phe-Gly-OH:

Boc-Gly-Gly-Phe-Gly-OH TFA Get quote

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Biological Activity
Purity & Documentation
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Description

Boc-Gly-Gly-Phe-Gly-OH, a self-assembly of N- and C-protected tetrapeptide, is a protease cleavable linker used for the antibody-drug conjugate (ADC).

IC₅₀ & Target

Cleavable Linker

Molecular Weight

436.46

Formula

C₂₀H₂₈N₄O₇

CAS No.

187794-49-6

Appearance

Solid

Color

White to off-white

Sequence Shortening

Boc-GGFG

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Sealed storage, away from moisture

Powder	-80°C	2 years
	-20°C	1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

DMSO : ≥ 100 mg/mL (229.12 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 25 mg/mL (57.28 mM; Need ultrasonic)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.2912 mL	11.4558 mL	22.9116 mL
5 mM	0.4582 mL	2.2912 mL	4.5823 mL
10 mM	0.2291 mL	1.1456 mL	2.2912 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (5.73 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (5.73 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 33.33 mg/mL (76.36 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.62%

Select Batch:

Data Sheet (246 KB) SDS (394 KB)

COA (248 KB) HNMR (274 KB) LCMS (91 KB) Elemental Analysis Report (108 KB)

Handling Instructions (2659 KB)

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O / DMSO	1 mM	2.2912 mL	11.4558 mL	22.9116 mL	57.2790 mL
	5 mM	0.4582 mL	2.2912 mL	4.5823 mL	11.4558 mL
	10 mM	0.2291 mL	1.1456 mL	2.2912 mL	5.7279 mL
	15 mM	0.1527 mL	0.7637 mL	1.5274 mL	3.8186 mL
	20 mM	0.1146 mL	0.5728 mL	1.1456 mL	2.8640 mL
	25 mM	0.0916 mL	0.4582 mL	0.9165 mL	2.2912 mL
	30 mM	0.0764 mL	0.3819 mL	0.7637 mL	1.9093 mL
	40 mM	0.0573 mL	0.2864 mL	0.5728 mL	1.4320 mL
	50 mM	0.0458 mL	0.2291 mL	0.4582 mL	1.1456 mL
DMSO	60 mM	0.0382 mL	0.1909 mL	0.3819 mL	0.9547 mL
	80 mM	0.0286 mL	0.1432 mL	0.2864 mL	0.7160 mL
	100 mM	0.0229 mL	0.1146 mL	0.2291 mL	0.5728 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.