{Boc}-Phe-Leu-Phe-Leu-Phe

Name: {Boc}-Phe-Leu-Phe-Leu-Phe
Brand: MedChemExpress
SKU: HY-P2355
Rating: 4.5 (1 reviews)

Cat. No.: HY-P2355 Purity: 99.50%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

{Boc}-Phe-Leu-Phe-Leu-Phe ({Boc}-FLFLF) is a formyl peptide receptor (FPR) family antagonist that preferentially inhibits activity triggered through the formyl peptide receptor.

For research use only. We do not sell to patients.

Custom Peptide Synthesis

{Boc}-Phe-Leu-Phe-Leu-Phe Chemical Structure

CAS No. : 66556-73-8

Size	Price	Stock	Quantity
5 mg	USD 150	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 240	In-stock	Estimated Time of Arrival: December 31
50 mg		Get quote
100 mg		Get quote

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Based on 1 publication(s) in Google Scholar

Other Forms of {Boc}-Phe-Leu-Phe-Leu-Phe:

{Boc}-Phe-Leu-Phe-Leu-Phe TFA Get quote

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Biological Activity
Purity & Documentation
References
Customer Review

Description

{Boc}-Phe-Leu-Phe-Leu-Phe ({Boc}-FLFLF) is a formyl peptide receptor (FPR) family antagonist that preferentially inhibits activity triggered through the formyl peptide receptor^[1].

Cellular Effect

Cell Line	Type	Value	Description	References
HL-60	IC₅₀	2000 nM Compound: 1	Antagonist activity at formyl peptide receptor in human HL60 cells assessed as inhibition of fMLP-induced calcium mobilization incubated for 5 mins prior to fMLP challenge by Fura-2 method Antagonist activity at formyl peptide receptor in human HL60 cells assessed as inhibition of fMLP-induced calcium mobilization incubated for 5 mins prior to fMLP challenge by Fura-2 method	[PMID: 25035262]

Molecular Weight

785.97

Formula

C₄₄H₅₉N₅O₈

CAS No.

66556-73-8

Appearance

Solid

Color

White to off-white

Sequence Shortening

{Boc}-FLFLF

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Sealed storage, away from moisture and light, under nitrogen

Powder	-80°C	2 years
	-20°C	1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen)

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (127.23 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.2723 mL	6.3616 mL	12.7231 mL
5 mM	0.2545 mL	1.2723 mL	2.5446 mL
10 mM	0.1272 mL	0.6362 mL	1.2723 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: 2.5 mg/mL (3.18 mM); Clear solution; Need ultrasonic

This protocol yields a clear solution of 2.5 mg/mL. If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.50%

Select Batch:

Data Sheet (272 KB) SDS (252 KB)

COA (249 KB) LCMS (87 KB)

Handling Instructions (2659 KB)

References

[1]. Anna-Lena Stenfeldt, et al. Cyclosporin H, Boc-MLF and Boc-FLFLF are antagonists that preferentially inhibit activity triggered through the formyl peptide receptor. Inflammation. 2007 Dec;30(6):224-9. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	1.2723 mL	6.3616 mL	12.7231 mL	31.8078 mL
	5 mM	0.2545 mL	1.2723 mL	2.5446 mL	6.3616 mL
	10 mM	0.1272 mL	0.6362 mL	1.2723 mL	3.1808 mL
	15 mM	0.0848 mL	0.4241 mL	0.8482 mL	2.1205 mL
	20 mM	0.0636 mL	0.3181 mL	0.6362 mL	1.5904 mL
	25 mM	0.0509 mL	0.2545 mL	0.5089 mL	1.2723 mL
	30 mM	0.0424 mL	0.2121 mL	0.4241 mL	1.0603 mL
	40 mM	0.0318 mL	0.1590 mL	0.3181 mL	0.7952 mL
	50 mM	0.0254 mL	0.1272 mL	0.2545 mL	0.6362 mL
	60 mM	0.0212 mL	0.1060 mL	0.2121 mL	0.5301 mL
	80 mM	0.0159 mL	0.0795 mL	0.1590 mL	0.3976 mL
	100 mM	0.0127 mL	0.0636 mL	0.1272 mL	0.3181 mL