Boc-Val-Pro-Arg-AMC hydrochloride

Name: Boc-Val-Pro-Arg-AMC hydrochloride
Brand: MedChemExpress
SKU: HY-137784
Rating: 4.5 (1 reviews)

Cat. No.: HY-137784 Purity: 99.31%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Boc-Val-Pro-Arg-AMC hydrochloride is a sensitive fluorogenic substrate for measuring trypsin-like serine proteases activity.

For research use only. We do not sell to patients.

Boc-Val-Pro-Arg-AMC hydrochloride Chemical Structure

CAS No. : 70375-24-5

Size	Price	Stock	Quantity
5 mg	USD 150	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 250	In-stock	Estimated Time of Arrival: December 31
50 mg		Get quote
100 mg		Get quote

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Based on 1 publication(s) in Google Scholar

Other Forms of Boc-Val-Pro-Arg-AMC hydrochloride:

Boc-Val-Pro-Arg-AMC Get quote

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Biological Activity
Purity & Documentation
References
Customer Review

Description

Boc-Val-Pro-Arg-AMC hydrochloride is a sensitive fluorogenic substrate for measuring trypsin-like serine proteases activity^[1].

In Vitro

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
Trypsin activity assay^[2]
1. Add diluted Trypsin enzyme (100 μL) to 800 μL of 50 mM Tris–HCl buffer (pH 8.0).
2. The reaction is immediately initiated by the addition of 100 μL of 50 μM substrate Boc-Val-Pro-Arg-AMC.
3. Incubate at 55 °C for 10 min.
4. Add 1.5 mL of the stopping agent (methyl alcohol:n-butyl alcohol:distilled water = 35:30:35, v/v/v) to stop the reaction.
5. Measure the fluorescence intensity by a spectrofluorometer (excitation 380 nm, emission 450 nm).

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

664.19

Formula

C₃₁H₄₆ClN₇O₇

CAS No.

70375-24-5

Appearance

Solid

Color

White to off-white

SMILES

CC(C(C(O1)=C2)=CC=C2NC([C@H](CCCNC(N)=N)NC([C@H](CCC3)N3C([C@H](C(C)C)NC(OC(C)(C)C)=O)=O)=O)=O)=CC1=O.Cl

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

DMSO : 17 mg/mL (25.60 mM; Need ultrasonic and warming; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 10 mg/mL (15.06 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.5056 mL	7.5280 mL	15.0559 mL
5 mM	0.3011 mL	1.5056 mL	3.0112 mL
10 mM	0.1506 mL	0.7528 mL	1.5056 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: 99.31%

Select Batch:

Data Sheet (274 KB) SDS (393 KB)

COA (252 KB) RP-HPLC (110 KB) MS (205 KB) Elemental Analysis Report (111 KB)

Handling Instructions (2659 KB)

References

[1]. Liu JY, et al. Purification and characterization of a sarcoplasmic serine proteinase from threadfin bream Nemipterus virgatus muscle. Food Chem. 2019 Jun 30;284:198-204. [Content Brief]

[2]. Sriket C, et al. Low molecular weight trypsin from hepatopancreas of freshwater prawn (Macrobrachium rosenbergii): Characteristics and biochemical properties. Food Chemistry, 2012, 134(1): 351-358.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O / DMSO	1 mM	1.5056 mL	7.5280 mL	15.0559 mL	37.6398 mL
	5 mM	0.3011 mL	1.5056 mL	3.0112 mL	7.5280 mL
	10 mM	0.1506 mL	0.7528 mL	1.5056 mL	3.7640 mL
	15 mM	0.1004 mL	0.5019 mL	1.0037 mL	2.5093 mL
DMSO	20 mM	0.0753 mL	0.3764 mL	0.7528 mL	1.8820 mL
DMSO	25 mM	0.0602 mL	0.3011 mL	0.6022 mL	1.5056 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.