Brassinin (Synonyms: Brassinine)

Name: Brassinin
Brand: MedChemExpress
SKU: HY-111334
Rating: 4.5 (1 reviews)

Cat. No.: HY-111334 Purity: 99.74%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Brassinin is the metabolism of a phytoalexin from Beassica pekinensis (Lour. ) Rupr..

For research use only. We do not sell to patients.

Brassinin Chemical Structure

CAS No. : 105748-59-2

Size	Price	Stock	Quantity
5 mg	USD 70	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 120	In-stock	Estimated Time of Arrival: December 31
50 mg		Get quote
100 mg		Get quote

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Based on 1 publication(s) in Google Scholar

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Description

Brassinin is the metabolism of a phytoalexin from Beassica pekinensis (Lour. ) Rupr.^[1].

Cellular Effect

Cell Line	Type	Value	Description	References
A549	IC₅₀	> 100 μM Compound: 1	Antiproliferative activity against human A549 cells assessed as cell viability after 72 hrs by MTT assay Antiproliferative activity against human A549 cells assessed as cell viability after 72 hrs by MTT assay	[PMID: 24012378]
CCRF-CEM	IC₅₀	90.2 μM Compound: 1	Antiproliferative activity against human CCRF-CEM cells assessed as cell viability after 72 hrs by MTT assay Antiproliferative activity against human CCRF-CEM cells assessed as cell viability after 72 hrs by MTT assay	[PMID: 24012378]
HeLa	IC₅₀	> 100 μM Compound: 1	Antiproliferative activity against human HeLa cells assessed as cell viability after 72 hrs by MTT assay Antiproliferative activity against human HeLa cells assessed as cell viability after 72 hrs by MTT assay	[PMID: 24012378]
Jurkat	IC₅₀	> 100 μM Compound: 1	Antiproliferative activity against human Jurkat cells assessed as cell viability after 72 hrs by MTT assay Antiproliferative activity against human Jurkat cells assessed as cell viability after 72 hrs by MTT assay	[PMID: 24012378]
MCF7	IC₅₀	> 100 μM Compound: 1	Antiproliferative activity against human MCF7 cells assessed as cell viability after 72 hrs by MTT assay Antiproliferative activity against human MCF7 cells assessed as cell viability after 72 hrs by MTT assay	[PMID: 24012378]
MDA-MB-231	IC₅₀	> 100 μM Compound: 1	Antiproliferative activity against human MDA-MB-231 cells assessed as cell viability after 72 hrs by MTT assay Antiproliferative activity against human MDA-MB-231 cells assessed as cell viability after 72 hrs by MTT assay	[PMID: 24012378]

Molecular Weight

236.36

Formula

C₁₁H₁₂N₂S₂

CAS No.

105748-59-2

Appearance

Solid

Color

Off-white to light yellow

SMILES

S=C(NCC1=CNC2=CC=CC=C21)SC

Structure Classification

Initial Source

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility

In Vitro:

DMSO : 50 mg/mL (211.54 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	4.2308 mL	21.1542 mL	42.3083 mL
5 mM	0.8462 mL	4.2308 mL	8.4617 mL
10 mM	0.4231 mL	2.1154 mL	4.2308 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: 1.25 mg/mL (5.29 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 1.25 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 1.25 mg/mL (5.29 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 1.25 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.74%

Select Batch:

Data Sheet (274 KB) SDS (252 KB)

COA (253 KB) HNMR (257 KB) LCMS (91 KB)

Handling Instructions (2659 KB)

References

[1]. M. Soledade C. Pedras, et al. Metabolic transformation of the phytoalexin brassinin by the "blackleg" fungus.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	4.2308 mL	21.1542 mL	42.3083 mL	105.7709 mL
	5 mM	0.8462 mL	4.2308 mL	8.4617 mL	21.1542 mL
	10 mM	0.4231 mL	2.1154 mL	4.2308 mL	10.5771 mL
	15 mM	0.2821 mL	1.4103 mL	2.8206 mL	7.0514 mL
	20 mM	0.2115 mL	1.0577 mL	2.1154 mL	5.2885 mL
	25 mM	0.1692 mL	0.8462 mL	1.6923 mL	4.2308 mL
	30 mM	0.1410 mL	0.7051 mL	1.4103 mL	3.5257 mL
	40 mM	0.1058 mL	0.5289 mL	1.0577 mL	2.6443 mL
	50 mM	0.0846 mL	0.4231 mL	0.8462 mL	2.1154 mL
	60 mM	0.0705 mL	0.3526 mL	0.7051 mL	1.7628 mL
	80 mM	0.0529 mL	0.2644 mL	0.5289 mL	1.3221 mL
	100 mM	0.0423 mL	0.2115 mL	0.4231 mL	1.0577 mL

Brassinin Related Classifications

Others

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Brassinin105748-59-2BrassinineOthersInhibitorinhibitorinhibit

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Brassinin (Synonyms: Brassinine)

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Complete Stock Solution Preparation Table

Brassinin Related Classifications

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