BZiPAR 

BZiPAR is a fluorescent probe. BZiPAR also is a substrate of trypsin that becomes fluorescent after cleavage by the protease^[1].

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
Confocal Imaging of Trypsinogen Activation:
1. Incubate the cells according to your normal protocol.
2. Isolate clusters of acinar cells.
3. Incubate acini in extracellular solution containing 100 μM BZiPAR.
4. BZiPAR were excited with a 488-nm laser line; emission was collected in the 508- to 530-nm band for cleaved BZiPAR.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

1404.44

C₇₀H₈₈Cl₂N₁₄O₁₃

254451-46-2

Solid

Off-white to yellow

CC[C@@H](C)[C@H](NC(OCC1=CC=CC=C1)=O)C(N2[C@@H](CCC2)C(N[C@H](C(NC3=CC=C(C(C(C=CC=C4)=C4C5=O)(O5)C6=C7C=C(NC([C@@H](NC([C@@H]8CCCN8C([C@@H](NC(OCC9=CC=CC=C9)=O)[C@H](C)CC)=O)=O)CCCNC(N)=N)=O)C=C6)C(O7)=C3)=O)CCCNC(N)=N)=O)=O.[H]Cl.[H]Cl

Room temperature in continental US; may vary elsewhere.

-20°C, protect from light, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

• [1]. Mark W Sherwood, et al. Activation of trypsinogen in large endocytic vacuoles of pancreatic acinar cells. Proc Natl Acad Sci U S A. 2007 Mar 27;104(13):5674-9.  [Content Brief]