2. Apoptosis Cell Cycle/DNA Damage
  3. Apoptosis CDK
  4. CDK9-IN-33

CDK9-IN-33 (compound C35) is a potent, selective and orally active CDK9 inhibitor with IC50 values of 17.44, 160, 316.30, 1771.00, >10000 nM for CDK9, CDK7, CDK2, CDK4, CDK6 respectively. CDK9-IN-33 induces apoptosis. CDK9-IN-33 decreases the protein expression of RPB1 CTD Ser2, RPB1, MCL1. CDK9-IN-33 shows anti-tumor activity.

For research use only. We do not sell to patients.

CDK9-IN-33 Chemical Structure

CDK9-IN-33 Chemical Structure

Size Stock
50 mg   Get quote  
100 mg   Get quote  
250 mg   Get quote  
Synthetic products have potential research and development risk.

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

CDK9-IN-33 Related Antibodies

Top Publications Citing Use of Products

View All CDK Isoform Specific Products:

View All Isoforms
CDK1 CDK2 CDK3 CDK4 CDK5 CDK6 CDK7 CDK8 CDK9 CDK11 CDK12 CDK13 CDK14 CDK16 CDK19 CDC CLK Pho85

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

CDK9-IN-33 (compound C35) is a potent, selective and orally active CDK9 inhibitor with IC50 values of 17.44, 160, 316.30, 1771.00, >10000 nM for CDK9, CDK7, CDK2, CDK4, CDK6 respectively. CDK9-IN-33 induces apoptosis. CDK9-IN-33 decreases the protein expression of RPB1 CTD Ser2, RPB1, MCL1. CDK9-IN-33 shows anti-tumor activity[1].

IC50 & Target[1]

CDK9

17.44 nM (IC50)

CDK7

160 nM (IC50)

CDK2

316.30 nM (IC50)

CDK4

1771.00 nM (IC50)

CDK6

>10000 nM (IC50)

In Vitro

CDK9-IN-33 (compound C35) (0, 0.01, 0.1, 1 µM; 24 h) decreases the protein expression of RPB1 CTD Ser2, RPB1, MCL1 and increases the expression of cleaved PARP[1].
CDK9-IN-33 (0, 0.01, 0.1, 1 µM) decreases the mRNA expression levels of the MCL1 gene[1].
. CDK9-IN-33 (0, 0.01, 0.1, 1 µM; 72 h) induces apoptosis in a dose-dependent manner[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

CDK9-IN-33 Related Antibodies

Cell Proliferation Assay[1]

Cell Line: MDA-MB-231, MDA-MB-468, BT549, 4T1, mino, MM.1S cells
Concentration:
Incubation Time: 4 days
Result: Showed antiproliferation potential for MDA-MB-231, MDA-MB-468, BT549, 4T1, mino, MM.1S cells with IC50 values of 5.9, 10.05, 5.61, 20.08, 8.98, 24.38 nM, respectively.

Western Blot Analysis[1]

Cell Line: MDA-MB-231 cells
Concentration: 0, 0.01, 0.1, 1 µM
Incubation Time: 24 h
Result: Showed a dose-dependent inhibition on the phosphorylation of RPB1 CTD Ser2, RPB1, increased the protein of cleaved PARP.

RT-PCR[1]

Cell Line: MDA-MB-231 cells
Concentration: 0, 0.01, 0.1, 1 µM
Incubation Time: 24 h
Result: Showed a dose-dependent inhibition on the phosphorylation of RPB1 CTD Ser2, RPB1, increased the protein of cleaved PARP.

Apoptosis Analysis[1]

Cell Line: 4T1 cells
Concentration: 0, 0.01, 0.1, 1 µM
Incubation Time: 72 h
Result: Induced apoptosis in a dose-dependent manner.
In Vivo

CDK9-IN-33 (10 mg/kg; p.o.; daily for 10 days) shows anti-tumor activity in mice[1].
Pharmacokinetic Parameters (male ICR mice; p.o. 20.0 mg/kg, i.v. 5.0 mg/kg; )[1].

Cmpd T1/2 (h) Tmax (h) Cmax (ng/mL) AUC0-t (h·ng/mL) AUCt–∞ (h·ng/mL) CL (mL/min/kg) MRT0–∞ (h) Vss (mL/kg) F (%)
C35 (p.o.) 0.68 0.5 2867 4349 4350 - 1.43 - 68.9
C35 (i.v.) 0.36 - - 1578 1578 52.9 0.33 1033 -

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Molecular Weight

437.54

Formula

C23H31N7O2
SMILES

CC(C1=C2N=C(N=C(N2N=C1)NCC3=CC=C(C=C3)NC(CC)=O)NC4CCOCC4)C
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
  • No file chosen (Maximum size is: 1024 Kb)
  • If you have published this work, please enter the PubMed ID.
  • Your name will appear on the site.

CDK9-IN-33 Related Classifications

  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass   Concentration   Volume   Molecular Weight *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

CDK9-IN-33ApoptosisCDKCyclin dependent kinaseorally activeantiproliferationRPB1 CTD Ser2RPB1MCL1MDA-MB-231 cellsapoptosisanti-tumorInhibitorinhibitorinhibit

Your Recently Viewed Products:

Inquiry Online

Your information is safe with us. * Required Fields.

Product Name

  

Salutation

Applicant Name *

 

Email Address *

Phone Number *

 

Organization Name *

Department *

 

Requested quantity *

Country or Region *

      

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
CDK9-IN-33
Cat. No.:
HY-162619
Quantity:
MCE Japan Authorized Agent:

Customer Review

Thank You for Your Feedback!

Request for HNMR Report

Please fill out this form to request the QC report. We will send it to your Email address shortly.

Your information is safe with us. * Required Fields.

Product Name

  

Lot #

Applicant Name *

 

Email Address *

Phone Number

 

Department *

Organization Name *

 

Country or Region *

Remarks

Request for HNMR Report

We have received your request and will respond to you as soon as possible.