CHM-1 (Synonyms: NSC656158)

Name: CHM-1
Brand: MedChemExpress
SKU: HY-103257
Rating: 4.5 (1 reviews)

Cat. No.: HY-103257 Purity: 99.57%: FAQs
Data Sheet SDS COA Handling Instructions

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CHM-1, a microtubule-destabilizing agent, inhibits tubulin polymerization. CHM-1 is a potent and selective antimitotic antitumor activity against human hepatocellular carcinoma. CHM-1 induces growth inhibition and apoptosis via G₂-M phase arrest in human hepatocellular carcinoma cells by activation of Cdc2 kinase activity.

For research use only. We do not sell to patients.

CHM-1 Chemical Structure

CAS No. : 154554-41-3

Size	Price	Stock	Quantity
1 mg	USD 45	In-stock	Estimated Time of Arrival: December 31
5 mg	USD 115	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 165	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 300	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 450	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 650	In-stock	Estimated Time of Arrival: December 31
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500 mg		Get quote

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Based on 1 publication(s) in Google Scholar

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Description

IC₅₀ & Target

IC50: 0.75 μM (HA22T)^[1]

Cellular Effect

Cell Line	Type	Value	Description	References
HCT-116	IC₅₀	0.15 μM Compound: CHM-1	Cytotoxicity against human HCT116 cells after 48 hrs by MTT assay Cytotoxicity against human HCT116 cells after 48 hrs by MTT assay	[PMID: 20973552]
Hep 3B2	IC₅₀	0.13 μM Compound: CHM-1	Cytotoxicity against human Hep3B cells after 48 hrs by MTT assay Cytotoxicity against human Hep3B cells after 48 hrs by MTT assay	[PMID: 20973552]
HL-60	IC₅₀	0.08 μM Compound: CHM-1	Cytotoxicity against human HL60 cells after 48 hrs by MTT assay Cytotoxicity against human HL60 cells after 48 hrs by MTT assay	[PMID: 20973552]
NCI-H460	IC₅₀	0.14 μM Compound: CHM-1	Cytotoxicity against human H460 cells after 48 hrs by MTT assay Cytotoxicity against human H460 cells after 48 hrs by MTT assay	[PMID: 20973552]

In Vitro

CHM-1 (0-100μM; 24 hours) induces significant concentration-dependent growth inhibition in HA22T, Hep3B, and HepG2 cells, with the most potent effects observed in HA22T cells (IC₅₀ = 0.75 μM)^[1].
CHM-1 (0-10 μM; 24 hours) significantly increases the binding of cyclin B1 to Cdc2 in HA22T cells^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:	HA22T, Hep3B, and HepG2 cells
Concentration:	0-100 μM
Incubation Time:	24 hours
Result:	Induced G₂-M arrest of the cell cycle followed by apoptosis.

Western Blot Analysis^[1]

Cell Line:	HA22T cells
Concentration:	0-10 μM
Incubation Time:	24 hours
Result:	Induced change in expressed and phosphorylated status of G₂-M regulators in human hepatocellular carcinoma cells.

In Vivo

CHM-1 (10 mg/kg; I.p.) induces a dose-dependent inhibition of HA22T tumor growth^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Male severe combined immunodeficient mice (HA22T)^[1]
Dosage:	10 mg/kg
Administration:	I.p.
Result:	Induced a dose-dependent inhibition of HA22T tumor growth.

Molecular Weight

283.25

Formula

C₁₆H₁₀FNO₃

CAS No.

154554-41-3

Appearance

Solid

Color

Off-white to light yellow

SMILES

O=C1C=C(C2=CC=CC=C2F)NC3=C1C=C4C(OCO4)=C3

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 5 mg/mL (17.65 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : < 0.1 mg/mL (insoluble)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.5305 mL	17.6523 mL	35.3045 mL
5 mM	0.7061 mL	3.5305 mL	7.0609 mL
10 mM	0.3530 mL	1.7652 mL	3.5305 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

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(per animal)

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: 99.57%

Select Batch:

Data Sheet (276 KB) SDS (251 KB)

COA (251 KB) HNMR (250 KB) LCMS (93 KB)

Handling Instructions (2659 KB)

References

[1]. Wang SW, et al. CHM-1, a novel synthetic quinolone with potent and selective antimitotic antitumor activity against human hepatocellular carcinoma in vitro and in vivo. Mol Cancer Ther. 2008 Feb;7(2):350-60. [Content Brief]

[2]. Liu CW, et al. CHM-1, a novel microtubule-destabilizing agent exhibits antitumor activity via inducing the expression of SIRT2 in human breast cancer cells. Chem Biol Interact. 2018 Jun 1;289:98-108. [Content Brief]

[3]. Tsai AC, et al. CHM-1, a new vascular targeting agent, induces apoptosis of human umbilical vein endothelial cells via p53-mediated death receptor 5 up-regulation. J Biol Chem. 2010 Feb 19;285(8):5497-506. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	3.5305 mL	17.6523 mL	35.3045 mL	88.2613 mL
	5 mM	0.7061 mL	3.5305 mL	7.0609 mL	17.6523 mL
	10 mM	0.3530 mL	1.7652 mL	3.5305 mL	8.8261 mL
	15 mM	0.2354 mL	1.1768 mL	2.3536 mL	5.8841 mL

CHM-1 Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

CHM-1154554-41-3NSC656158CHM1CHM 1NSC 656158NSC-656158Microtubule/Tubulinmicrotubule-destabilizingantimitoticantitumorhepatocellularcarcinomaCdc2Inhibitorinhibitorinhibit

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CHM-1 (Synonyms: NSC656158)

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CHM-1 Related Antibodies

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Complete Stock Solution Preparation Table

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