Cirsilineol

Name: Cirsilineol
Brand: MedChemExpress
SKU: HY-119347
Rating: 5.0 (2 reviews)

Cat. No.: HY-119347 Purity: ≥98.0%: FAQs
Data Sheet SDS COA Handling Instructions

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Cirsilineol, a natural flavone compound, selectively inhibits IFN-γ/STAT1/T-bet signaling in intestinal CD4⁺ T cells. Cirsilineol has potent immunosuppressive and anti-tumor properties. Cirsilineol significantly ameliorates trinitro-benzene sulfonic acid (TNBS)-induced T-cell-mediated experimental colitis in mice.

For research use only. We do not sell to patients.

Cirsilineol Chemical Structure

CAS No. : 41365-32-6

Size	Price	Stock	Quantity
1 mg	USD 420	In-stock	Estimated Time of Arrival: December 31
5 mg	USD 1480	In-stock	Estimated Time of Arrival: December 31
10 mg		Get quote
50 mg		Get quote

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Customer Review

Based on 2 publication(s) in Google Scholar

Other Forms of Cirsilineol:

Cirsilineol (Standard) Get quote

2 Publications Citing Use of MCE Cirsilineol

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View All Isoforms

STAT3 STAT5 STAT6 STAT1

Biological Activity
Purity & Documentation
References
Customer Review

Description

Cellular Effect

Cell Line	Type	Value	Description	References
A2780	IC₅₀	33 μg/mL Compound: Cirsilineol	Cytotoxicity against human A2780 cells after 72 hrs by MTT assay Cytotoxicity against human A2780 cells after 72 hrs by MTT assay	[PMID: 31784199]
A-431	IC₅₀	33 μg/mL Compound: Cirsilineol	Cytotoxicity against human A431 cells after 72 hrs by MTT assay Cytotoxicity against human A431 cells after 72 hrs by MTT assay	[PMID: 31784199]
HeLa	IC₅₀	33 μg/mL Compound: Cirsilineol	Cytotoxicity against human HeLa cells after 72 hrs by MTT assay Cytotoxicity against human HeLa cells after 72 hrs by MTT assay	[PMID: 31784199]
MCF7	IC₅₀	33 μg/mL Compound: Cirsilineol	Cytotoxicity against human MCF7 cells after 72 hrs by MTT assay Cytotoxicity against human MCF7 cells after 72 hrs by MTT assay	[PMID: 31784199]

In Vitro

Cirsilineol (0.1-10 μM; 96 hours) inhibits single mixed lymphocyte reaction and Concanava A-induced T-cell proliferation in a dose-dependent manner. Cirsilineol (10 μM) does not affect T lymphocyte's viability. The inhibition of cirsilineol (10 μM) on T-cell proliferation is not due to a cytotoxic activity^[1].
Cirsilineol (1-10 μM; pretreatment for 3 hours) completely inhibits IFN-γ-induced Tyr701 phosphorylation of STAT1 and JAK2 activation^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Proliferation Assay^[1]

Cell Line:	Splenocytes
Concentration:	0.1, 1, 10 μM
Incubation Time:	96 hours
Result:	Inhibited single mixed lymphocyte reaction and Concanavalin A (Con A; 5 μg/mL; for 72 h)-induced T-cell proliferation in a dose-dependent manner. Suppressed OVA_323-339-specific CD4⁺ T-cell proliferation.

Western Blot Analysis^[1]

Cell Line:	Splenic CD4⁺ T cells
Concentration:	1, 5, 10 μM
Incubation Time:	Pretreatment for 3 hours
Result:	Completely inhibited IFN-γ-induced (25 ng; 30 mins) Tyr701 phosphorylation of STAT1 and JAK2 activation. Suppressed IFN-γ-induced (25 ng; 12 hours) Th1-specific transcription factor T-bet.

In Vivo

cirsilineol (3, 10, and 30 mg/kg) significantly ameliorates TNBS-induced Th1-mediated colitis through inhibiting IFN-γ/STAT1/T-bet signaling in CD4⁺ T cells.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	8-10-week-old female C57BL/6, BALB/c and DO11.10 transgenic mice with TNBS (10 mg; 100 μL)^[1]
Dosage:	3, 10, 30 mg/kg
Administration:	IP; daily; for 11 days
Result:	Showed a significant improved effect on the body weights and survival rate of mice. Markedly reduced inflammatory infiltration, restoration of the destructive mucosal architecture and remission of edema.

Molecular Weight

344.32

Formula

C₁₈H₁₆O₇

CAS No.

41365-32-6

Appearance

Solid

Color

White to light yellow

SMILES

O=C1C=C(OC2=CC(OC)=C(C(O)=C12)OC)C3=CC=C(C(OC)=C3)O

Structure Classification

Initial Source

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility

In Vitro:

DMSO : 3.5 mg/mL (10.16 mM; Need ultrasonic and warming; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.9043 mL	14.5214 mL	29.0428 mL
5 mM	0.5809 mL	2.9043 mL	5.8085 mL
10 mM	0.2904 mL	1.4521 mL	2.9043 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

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In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: ≥98.0%

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Data Sheet (276 KB) SDS (252 KB)

COA (249 KB)

Handling Instructions (2659 KB)

References

[1]. Yang Sun, et al. Novel immunomodulatory properties of cirsilineol through selective inhibition of IFN-gamma signaling in a murine model of inflammatory bowel disease. Biochem Pharmacol. 2010 Jan 15;79(2):229-38. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.9043 mL	14.5214 mL	29.0428 mL	72.6069 mL
	5 mM	0.5809 mL	2.9043 mL	5.8086 mL	14.5214 mL
	10 mM	0.2904 mL	1.4521 mL	2.9043 mL	7.2607 mL

Cirsilineol Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Cirsilineol41365-32-6IFNARSTATInterferon-α/β receptorInterferon-alpha/beta receptorIFN-γSTAT1T-betCD4+T cellsimmunosuppressiveTNBScolitisSplenocytesInhibitorinhibitorinhibit

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Cirsilineol

Customer Review

Other Forms of Cirsilineol:

Cirsilineol Related Antibodies

2 Publications Citing Use of MCE Cirsilineol

Featured Recommendations

•Related Small Molecules:

•Related Proteins:

View All STAT Isoform Specific Products:

Biological Activity

Purity & Documentation

References

Customer Review

Cirsilineol Related Antibodies

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Complete Stock Solution Preparation Table

Cirsilineol Related Classifications

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