Citric acid-¹³C₆

Name: Citric acid- 13 C 6
Brand: MedChemExpress
SKU: HY-N1428S1
Rating: 4.5 (1 reviews)

Cat. No.: HY-N1428S1 Purity: ≥99.0%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Citric acid-¹³C₆ is the ¹³C-labeled Citric acid. Citric acid is a weak organic tricarboxylic acid found in citrus fruits. Citric acid is a natural preservative and food tartness enhancer.

For research use only. We do not sell to patients.

Citric acid-<sup>13</sup>C<sub>6</sub> Chemical Structure

Citric acid-¹³C₆ Chemical Structure

CAS No. : 287389-42-8

Size	Price	Stock	Quantity
1 mg	USD 130	In-stock	Estimated Time of Arrival: December 31
5 mg	USD 460	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 780	In-stock	Estimated Time of Arrival: December 31
50 mg		Get quote
100 mg		Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of Citric acid-¹³C₆:

Citric acid In-stock

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Biological Activity
Purity & Documentation
References
Customer Review

Description

Citric acid-¹³C₆ is the ¹³C-labeled Citric acid. Citric acid is a weak organic tricarboxylic acid found in citrus fruits. Citric acid is a natural preservative and food tartness enhancer.

In Vitro

Stable heavy isotopes of hydrogen, carbon, and other elements have been incorporated into drug molecules, largely as tracers for quantitation during the drug development process. Deuteration has gained attention because of its potential to affect the pharmacokinetic and metabolic profiles of drugs^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

198.08

Formula

¹³C₆H₈O₇

CAS No.

287389-42-8

Unlabeled CAS

77-92-9

Appearance

Solid

Color

White to light yellow

SMILES

O[13C]([13CH2][13C]([13C](O)=O)(O)[13CH2][13C](O)=O)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

H₂O : 100 mg/mL (504.85 mM; Need ultrasonic)

DMSO : 100 mg/mL (504.85 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	5.0485 mL	25.2423 mL	50.4847 mL
5 mM	1.0097 mL	5.0485 mL	10.0969 mL
10 mM	0.5048 mL	2.5242 mL	5.0485 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (12.62 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 2.5 mg/mL (12.62 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: ≥99.0%

Select Batch:

Data Sheet (274 KB) SDS (393 KB)

COA (249 KB)

Handling Instructions (2659 KB)

References

[1]. Russak EM, et al. Impact of Deuterium Substitution on the Pharmacokinetics of Pharmaceuticals. Ann Pharmacother. 2019;53(2):211-216. [Content Brief]

[2]. Ying TH, et al. Citric acid induces cell-cycle arrest and apoptosis of human immortalized keratinocyte cell line (HaCaT) via caspase- and mitochondrial-dependent signaling pathways. Anticancer Res. 2013 Oct;33(10):4411-20. [Content Brief]

[3]. Abdel-Salam OM, et al. Citric acid effects on brain and liver oxidative stress in lipopolysaccharide-treated mice. J Med Food. 2014 May;17(5):588-98. [Content Brief]

[4]. Lacour B, et al. Stimulation by citric acid of calcium and phosphorus bioavailability in rats fed a calcium-rich diet. Miner Electrolyte Metab. 1997;23(2):79-87. [Content Brief]

[5]. Nagai R, et al. Citric acid inhibits development of cataracts, proteinuria and ketosis in streptozotocin (type 1) diabetic rats. Biochem Biophys Res Commun. 2010 Feb 26;393(1):118-22. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O / H₂O / DMSO / DMSO	1 mM	5.0485 mL	25.2423 mL	50.4847 mL	126.2116 mL
	5 mM	1.0097 mL	5.0485 mL	10.0969 mL	25.2423 mL
	10 mM	0.5048 mL	2.5242 mL	5.0485 mL	12.6212 mL
	15 mM	0.3366 mL	1.6828 mL	3.3656 mL	8.4141 mL
	20 mM	0.2524 mL	1.2621 mL	2.5242 mL	6.3106 mL
	25 mM	0.2019 mL	1.0097 mL	2.0194 mL	5.0485 mL
	30 mM	0.1683 mL	0.8414 mL	1.6828 mL	4.2071 mL
	40 mM	0.1262 mL	0.6311 mL	1.2621 mL	3.1553 mL
	50 mM	0.1010 mL	0.5048 mL	1.0097 mL	2.5242 mL
	60 mM	0.0841 mL	0.4207 mL	0.8414 mL	2.1035 mL
	80 mM	0.0631 mL	0.3155 mL	0.6311 mL	1.5776 mL
	100 mM	0.0505 mL	0.2524 mL	0.5048 mL	1.2621 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.