Clobetasone butyrate

Name: Clobetasone butyrate
Brand: MedChemExpress
SKU: HY-B1616
Rating: 4.5 (1 reviews)

Cat. No.: HY-B1616 Purity: 99.80%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Clobetasone butyrate is a synthetic glucocorticoid and has topical anti-inflammatory activity especially in skin. Clobetasone butyrate can be used to relieve corticosteroid-responsive dermatoses, including atopic?dermatitis and psoriasis.

For research use only. We do not sell to patients.

Clobetasone butyrate Chemical Structure

CAS No. : 25122-57-0

Size	Price	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 105	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 105	In-stock	Estimated Time of Arrival: December 31
Solid
100 mg	USD 95	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

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This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of Clobetasone butyrate:

Clobetasone butyrate (Standard) Get quote

1 Publications Citing Use of MCE Clobetasone butyrate

•J Am Chem Soc. 2021 Oct 14. [Abstract]

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Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target

IC50: glucocorticoid^[1]

In Vivo

Clobetasone butyrate (application on the skin; 0.05%; 7 weeks) induces epidermal thinning in skin, Clobetasone butyrate 7 weeks causes much less epidermal thinning in the pig than flurandrenolone (0.05%) or fluocinolone acetonide (0.025%), both of which causes severe atrophy^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Large white domestic pig^[1]
Dosage:	0.5% application on the skin
Administration:	7 weeks
Result:	Caused moderate to slight atrophy.

Clinical Trial

Molecular Weight

478.98

Formula

C₂₆H₃₂ClFO₅

CAS No.

25122-57-0

Appearance

Solid

Color

White to off-white

SMILES

C[C@@]1(C2)[C@](C(CCl)=O)(OC(CCC)=O)[C@@H](C)C[C@@]1([H])[C@]3([H])CCC4=CC(C=C[C@]4(C)[C@@]3(F)C2=O)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (208.78 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.0878 mL	10.4388 mL	20.8777 mL
5 mM	0.4176 mL	2.0878 mL	4.1755 mL
10 mM	0.2088 mL	1.0439 mL	2.0878 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 5 mg/mL (10.44 mM); Clear solution

This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.80%

Select Batch:

Data Sheet (278 KB) SDS (418 KB)

COA (256 KB) HNMR (264 KB) LCMS (93 KB)

Handling Instructions (2659 KB)

References

[1]. Munro DD, et al. Clobetasone butyrate, a new topical corticosteroid: clinical activity and effects on pituitary-adrenal axis function and model of epidermal atrophy. [Content Brief]

[2]. Michael Stewart, et al. Clobetasone butyrate for inflammatory skin conditions.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.0878 mL	10.4388 mL	20.8777 mL	52.1942 mL
	5 mM	0.4176 mL	2.0878 mL	4.1755 mL	10.4388 mL
	10 mM	0.2088 mL	1.0439 mL	2.0878 mL	5.2194 mL
	15 mM	0.1392 mL	0.6959 mL	1.3918 mL	3.4796 mL
	20 mM	0.1044 mL	0.5219 mL	1.0439 mL	2.6097 mL
	25 mM	0.0835 mL	0.4176 mL	0.8351 mL	2.0878 mL
	30 mM	0.0696 mL	0.3480 mL	0.6959 mL	1.7398 mL
	40 mM	0.0522 mL	0.2610 mL	0.5219 mL	1.3049 mL
	50 mM	0.0418 mL	0.2088 mL	0.4176 mL	1.0439 mL
	60 mM	0.0348 mL	0.1740 mL	0.3480 mL	0.8699 mL
	80 mM	0.0261 mL	0.1305 mL	0.2610 mL	0.6524 mL
	100 mM	0.0209 mL	0.1044 mL	0.2088 mL	0.5219 mL