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  3. Fluorescent Dye
  4. Coumarin 6H

Coumarin 6H, a Coumarin (Coumarin (HY-N0709)) derivative, is a laser dye. The fluorescence emission of Coumarin 6H can be enhanced by hydrogen bonding interactions.

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Coumarin 6H Chemical Structure

Coumarin 6H Chemical Structure

CAS No. : 58336-35-9

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Based on 1 publication(s) in Google Scholar

Coumarin 6H Related Antibodies

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Description

Coumarin 6H, a Coumarin (Coumarin (HY-N0709)) derivative, is a laser dye. The fluorescence emission of Coumarin 6H can be enhanced by hydrogen bonding interactions[1][2].

In Vitro

The Coumarin 6H with twistblocked and stronger electron-donating julolidine group exhibits higher quantum yield, better biocompatibility, as well as less background fluorescence when suppressed by a fluorescence quenching group. Thus, the Coumarin 6H in probe LH-1 may help to reduce the background fluorescence. In probe LH-1, the fluorescence of Coumarin 6H fluorophore not only can be quenched by the rotation of N-N group, but also can be quenched by the 2,4-dinitrobenzenze group via donor-excited photoinduced electron transfer (d-PET) mechanism[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Coumarin 6H Related Antibodies
Molecular Weight

241.29

Formula

C15H15NO2
CAS No.
Appearance

Solid

Color

Brown to breen

Emission (Em)

454
Excitation (Ex)

344
SMILES

O=C1C=CC2=CC3=C4N(CCCC4=C2O1)CCC3
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 12.5 mg/mL (51.80 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 4.1444 mL 20.7220 mL 41.4439 mL
5 mM 0.8289 mL 4.1444 mL 8.2888 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
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Concentration
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Volume
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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

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Volume (start)

V1

=
Concentration (final)

C2

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Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 4.1444 mL 20.7220 mL 41.4439 mL 103.6098 mL
5 mM 0.8289 mL 4.1444 mL 8.2888 mL 20.7220 mL
10 mM 0.4144 mL 2.0722 mL 4.1444 mL 10.3610 mL
15 mM 0.2763 mL 1.3815 mL 2.7629 mL 6.9073 mL
20 mM 0.2072 mL 1.0361 mL 2.0722 mL 5.1805 mL
25 mM 0.1658 mL 0.8289 mL 1.6578 mL 4.1444 mL
30 mM 0.1381 mL 0.6907 mL 1.3815 mL 3.4537 mL
40 mM 0.1036 mL 0.5180 mL 1.0361 mL 2.5902 mL
50 mM 0.0829 mL 0.4144 mL 0.8289 mL 2.0722 mL
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Coumarin 6H Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Coumarin 6H58336-35-9Fluorescent Dyehydrogenlaser dyeInhibitorinhibitorinhibit

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