Coumarin

Name: Coumarin
Brand: MedChemExpress
SKU: HY-N0709
Rating: 4.5 (1 reviews)

Cat. No.: HY-N0709 Purity: 99.81%: FAQs
Data Sheet SDS COA Handling Instructions

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Coumarin is a potent and orally active anti-inflammatory agent. Coumarin shows an antinociceptive effect. Coumarin shows antibacterial, antifungal and anticancer activity.

For research use only. We do not sell to patients.

Coumarin Chemical Structure

CAS No. : 91-64-5

Size	Price	Stock	Quantity

Free Sample (0.1 - 0.5 mg)		Apply Now
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 66	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 66	In-stock	Estimated Time of Arrival: December 31
Solid
100 mg	USD 60	In-stock	Estimated Time of Arrival: December 31
500 mg	USD 96	In-stock	Estimated Time of Arrival: December 31
1 g		Get quote
5 g		Get quote

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Based on 1 publication(s) in Google Scholar

Other Forms of Coumarin:

Coumarin-d₄ Get quote
Coumarin-d₆ Get quote
Coumarin (Standard) Get quote

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Biological Activity
Purity & Documentation
References
Customer Review

Description

Coumarin is a potent and orally active anti-inflammatory agent. Coumarin shows an antinociceptive effect. Coumarin shows antibacterial, antifungal and anticancer activity^[1]^[2].

Cellular Effect

Cell Line	Type	Value	Description	References
A2780	IC₅₀	> 100 μM Compound: Coumarin	Anticancer activity against human A2780 cells by CCK8 assay Anticancer activity against human A2780 cells by CCK8 assay	[PMID: 31404864]
HEL	CC₅₀	> 500 μM Compound: Coumarin	Cytotoxicity against human HEL cells measured after 3 days by coulter counting method Cytotoxicity against human HEL cells measured after 3 days by coulter counting method	[PMID: 35176562]
HeLa	IC₅₀	> 25 μM Compound: 10	Inhibition of CRM1-mediated hemagglutininin-tagged HIV1 Rev protein nuclear export in human HeLa cells assessed as nucleus localization after 12 hrs by indirect fluorescent antibody technique Inhibition of CRM1-mediated hemagglutininin-tagged HIV1 Rev protein nuclear export in human HeLa cells assessed as nucleus localization after 12 hrs by indirect fluorescent antibody technique	[PMID: 20493693]
Hep 3B2	IC₅₀	> 100 μM Compound: Coumarin	Anticancer activity against human Hep3B cells by CCK8 assay Anticancer activity against human Hep3B cells by CCK8 assay	[PMID: 31404864]
HepG2	IC₅₀	> 100 μM Compound: Coumarin	Anticancer activity against human HepG2 cells by CCK8 assay Anticancer activity against human HepG2 cells by CCK8 assay	[PMID: 31404864]
HL-60	IC₅₀	> 50 μM Compound: Coumarin	Cytotoxicity against human HL60 cells after 72 hrs by WST1 assay in presence of 20% FBS Cytotoxicity against human HL60 cells after 72 hrs by WST1 assay in presence of 20% FBS	[PMID: 22901895]
MCF7	IC₅₀	> 50 μM Compound: Coumarin	Cytotoxicity against human MCF7 cells after 72 hrs by WST1 assay in presence of 20% FBS Cytotoxicity against human MCF7 cells after 72 hrs by WST1 assay in presence of 20% FBS	[PMID: 22901895]
N2a	IC₅₀	> 50 μM Compound: Coumarin	Cytotoxicity against mouse Neuro2a cells after 72 hrs by WST1 assay in presence of 20% FBS Cytotoxicity against mouse Neuro2a cells after 72 hrs by WST1 assay in presence of 20% FBS	[PMID: 22901895]
OVCAR-3	IC₅₀	> 100 μM Compound: Coumarin	Anticancer activity against human OVCAR3 cells by CCK8 assay Anticancer activity against human OVCAR3 cells by CCK8 assay	[PMID: 31404864]
U-937	CC₅₀	> 2 mM Compound: Coumarin	Cytotoxicity against human U937 cells after 48 hrs by trypan blue assay Cytotoxicity against human U937 cells after 48 hrs by trypan blue assay	[PMID: 19716307]
U-937	IC₅₀	> 2000 μM Compound: 1	Growth inhibition of human U937 cells by [3H]thymidine incorporation assay Growth inhibition of human U937 cells by [3H]thymidine incorporation assay	[PMID: 18060791]
U-937	CC₅₀	> 2000 μM Compound: 1	Cytotoxicity against human U937 cells by trypan blue assay after 48 hrs Cytotoxicity against human U937 cells by trypan blue assay after 48 hrs	[PMID: 18060791]
U-937	IC₅₀	> 400 μM Compound: Coumarin	Growth inhibition of human U937 cells assessed as [3H]5-methyl thymidine incorporation after 48 hrs by liquid scintillation counting Growth inhibition of human U937 cells assessed as [3H]5-methyl thymidine incorporation after 48 hrs by liquid scintillation counting	[PMID: 19716307]
U-937	IC₅₀	> 50 μM Compound: Coumarin	Cytotoxicity against human U937 cells after 72 hrs by WST1 assay in presence of 20% FBS Cytotoxicity against human U937 cells after 72 hrs by WST1 assay in presence of 20% FBS	[PMID: 22901895]

In Vivo

Coumarin (1-10 mg/kg; p.o.) shows an antinociceptive effect in a dose-dependent manner in the acetic acid-induced writhing test^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	20-25 g, Male ICR mice^[1]
Dosage:	1-10 mg/kg
Administration:	P.o.; 30 min prior to performing the acetic acid
Result:	Showed an antinociceptive effect in a dose-dependent manner as measured in the acetic acid-induced writhing test.

Molecular Weight

146.14

Formula

C₉H₆O₂

CAS No.

91-64-5

Appearance

Solid

Color

White to off-white

SMILES

O=C1C=CC2=CC=CC=C2O1

Structure Classification

Initial Source

Microorganisms

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Solvent & Solubility

In Vitro:

DMSO : ≥ 100 mg/mL (684.28 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 4 mg/mL (27.37 mM; Need ultrasonic)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	6.8428 mL	34.2138 mL	68.4275 mL
5 mM	1.3686 mL	6.8428 mL	13.6855 mL
10 mM	0.6843 mL	3.4214 mL	6.8428 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 3 mg/mL (20.53 mM); Clear solution

This protocol yields a clear solution of ≥ 3 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (30.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 3 mg/mL (20.53 mM); Clear solution

This protocol yields a clear solution of ≥ 3 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (30.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 3 mg/mL (20.53 mM); Clear solution

This protocol yields a clear solution of ≥ 3 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (30.0 mg/mL) to 900 μL Corn oil, and mix evenly.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 8.33 mg/mL (57.00 mM); Clear solution; Need ultrasonic and warming and heat to 60°C

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.81%

Select Batch:

Data Sheet (274 KB) SDS (480 KB)

COA (250 KB) LCMS (265 KB)

Handling Instructions (2659 KB)

References

[1]. Park SH, et al. Antinociceptive profiles and mechanisms of orally administered coumarin in mice. Biol Pharm Bull. 2013;36(6):925-30. [Content Brief]

[2]. enugopala KN, et al. Review on natural coumarin lead compounds for their pharmacological activity. Biomed Res Int. 2013;2013:963248. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O / DMSO	1 mM	6.8428 mL	34.2138 mL	68.4275 mL	171.0688 mL
	5 mM	1.3686 mL	6.8428 mL	13.6855 mL	34.2138 mL
	10 mM	0.6843 mL	3.4214 mL	6.8428 mL	17.1069 mL
	15 mM	0.4562 mL	2.2809 mL	4.5618 mL	11.4046 mL
	20 mM	0.3421 mL	1.7107 mL	3.4214 mL	8.5534 mL
	25 mM	0.2737 mL	1.3686 mL	2.7371 mL	6.8428 mL
DMSO	30 mM	0.2281 mL	1.1405 mL	2.2809 mL	5.7023 mL
	40 mM	0.1711 mL	0.8553 mL	1.7107 mL	4.2767 mL
	50 mM	0.1369 mL	0.6843 mL	1.3686 mL	3.4214 mL
	60 mM	0.1140 mL	0.5702 mL	1.1405 mL	2.8511 mL
	80 mM	0.0855 mL	0.4277 mL	0.8553 mL	2.1384 mL
	100 mM	0.0684 mL	0.3421 mL	0.6843 mL	1.7107 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Coumarin Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Coumarin91-64-5BacterialFungalantibacterialantifungalanticanceranti-inflammatoryantinociceptiveInhibitorinhibitorinhibit

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Coumarin

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Other Forms of Coumarin:

Coumarin Related Antibodies

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Complete Stock Solution Preparation Table

Coumarin Related Classifications

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