CP-31398 dihydrochloride

Name: CP-31398 dihydrochloride
Brand: MedChemExpress
SKU: HY-18343A
Rating: 4.5 (1 reviews)

Cat. No.: HY-18343A Purity: 99.63%: FAQs
Data Sheet SDS COA Handling Instructions

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CP-31398 dihydrochloride stabilizes the active conformation of p53 and promotes p53 activity in cancer cell lines with mutant or wild-type p53.

For research use only. We do not sell to patients.

CP-31398 dihydrochloride Chemical Structure

CAS No. : 1217195-61-3

Size	Price	Stock	Quantity
1 mg	USD 41	In-stock	Estimated Time of Arrival: December 31
5 mg	USD 88	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 143	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 275	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 440	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 715	Get quote	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of CP-31398 dihydrochloride:

CP-31398 Get quote

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Biological Activity
Purity & Documentation
References
Customer Review

Description

CP-31398 dihydrochloride stabilizes the active conformation of p53 and promotes p53 activity in cancer cell lines with mutant or wild-type p53^[1]^[2]^[3].

In Vitro

CP-31398 (36.75 μM, 16 h) induces p21 in p53-mutant cells^[1].
CP-31398 (15 μg/mL, 20 hours) could induce apoptosis and cell cycle arrest in SW480 cells^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[1]

Cell Line:	Saos-2 cells expressing transfected mutant p53.
Concentration:	36.75 μM.
Incubation Time:	16 h.
Result:	Induction of p21 in cells expressing only mutant p53.

Western Blot Analysis^[3]

Cell Line:	LN-18 and U87MG cells.
Concentration:	36 μM.
Incubation Time:	16 h.
Result:	Decreased the levels of procaspase 3 and induced cleavage of caspase 7.

In Vivo

CP-31398 (100 mg/kg, orally) exhibits significant anti-tumor activity in mice models^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Small human tumor xenografts in mice^[1].
Dosage:	100 mg/kg.
Administration:	Orally twice daily for 7 days.
Result:	Suppressed A375.S2 tumor growth by -50%.

Molecular Weight

435.39

Formula

C₂₂H₂₈Cl₂N₄O

CAS No.

1217195-61-3

Appearance

Solid

Color

Light yellow to yellow

SMILES

CN(C)CCCNC1=C2C=CC=CC2=NC(/C=C/C3=CC=C(OC)C=C3)=N1.[H]Cl.[H]Cl

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility

In Vitro:

DMSO : 120 mg/mL (275.61 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.2968 mL	11.4840 mL	22.9679 mL
5 mM	0.4594 mL	2.2968 mL	4.5936 mL
10 mM	0.2297 mL	1.1484 mL	2.2968 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 3 mg/mL (6.89 mM); Clear solution

This protocol yields a clear solution of ≥ 3 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (30.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 3 mg/mL (6.89 mM); Clear solution

This protocol yields a clear solution of ≥ 3 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (30.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.63%

Select Batch:

Data Sheet (273 KB) SDS (393 KB)

COA (245 KB) HNMR (254 KB) RP-HPLC (256 KB) LCMS (102 KB)

Handling Instructions (2659 KB)

References

[1]. B A Foster, et al. Pharmacological rescue of mutant p53 conformation and function. Science. 1999 Dec 24;286(5449):2507-10. [Content Brief]

[2]. Rishu Takimoto, et al. The Mutant p53-Conformation Modifying Drug, CP-31398, Can Induce Apoptosis of Human Cancer Cells and Can Stabilize Wild-Type p53 Protein. Cancer Biol Ther. Jan-Feb 2002;1(1):47-55. [Content Brief]

[3]. J Wischhusen, et al. CP-31398, a novel p53-stabilizing agent, induces p53-dependent and p53-independent glioma cell death. Oncogene. 2003 Nov 13;22(51):8233-45. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.2968 mL	11.4840 mL	22.9679 mL	57.4198 mL
	5 mM	0.4594 mL	2.2968 mL	4.5936 mL	11.4840 mL
	10 mM	0.2297 mL	1.1484 mL	2.2968 mL	5.7420 mL
	15 mM	0.1531 mL	0.7656 mL	1.5312 mL	3.8280 mL
	20 mM	0.1148 mL	0.5742 mL	1.1484 mL	2.8710 mL
	25 mM	0.0919 mL	0.4594 mL	0.9187 mL	2.2968 mL
	30 mM	0.0766 mL	0.3828 mL	0.7656 mL	1.9140 mL
	40 mM	0.0574 mL	0.2871 mL	0.5742 mL	1.4355 mL
	50 mM	0.0459 mL	0.2297 mL	0.4594 mL	1.1484 mL
	60 mM	0.0383 mL	0.1914 mL	0.3828 mL	0.9570 mL
	80 mM	0.0287 mL	0.1435 mL	0.2871 mL	0.7177 mL
	100 mM	0.0230 mL	0.1148 mL	0.2297 mL	0.5742 mL