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Cy5.5-SE TEA  (Synonyms: Cyanine5.5 NHS ester TEA)

Cat. No.: HY-D0925B Purity: 97.26%
Data Sheet Handling Instructions Technical Support

Cy5.5-SE TEA (Cyanine5.5 NHS ester TEA) is a CY dye. CY, short for Cyanine, is a compound consisting of two nitrogen atoms connected by an odd number of methyl units. Cyanine compounds have the characteristics of long wavelength, adjustable absorption and emission, high extinction coefficient, good water solubility and relatively simple synthesis. CY dyes are of en used for the labeling of proteins, antibodies and small molecular compounds. For the labeling of protein antibodies, the combination can be completed through a simple mixing reaction. Below, we introduce the labeling method of protein antibody labeling, which has certain reference significance. Storage: protect from light.

For research use only. We do not sell to patients.

Cy5.5-SE TEA Chemical Structure

Cy5.5-SE TEA Chemical Structure

Size Price Stock Quantity
5 mg USD 435 In-stock
10 mg USD 702 In-stock
25 mg USD 1476 In-stock
50 mg USD 2288 In-stock
100 mg USD 3500 In-stock
200 mg   Get quote  
500 mg   Get quote  

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Based on 1 publication(s) in Google Scholar

Other Forms of Cy5.5-SE TEA:

Top Publications Citing Use of Products

1 Publications Citing Use of MCE Cy5.5-SE TEA

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  • Purity & Documentation

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Description

Cy5.5-SE TEA (Cyanine5.5 NHS ester TEA) is a CY dye. CY, short for Cyanine, is a compound consisting of two nitrogen atoms connected by an odd number of methyl units. Cyanine compounds have the characteristics of long wavelength, adjustable absorption and emission, high extinction coefficient, good water solubility and relatively simple synthesis[1]. CY dyes are of en used for the labeling of proteins, antibodies and small molecular compounds. For the labeling of protein antibodies, the combination can be completed through a simple mixing reaction. Below, we introduce the labeling method of protein antibody labeling, which has certain reference significance[2]. Storage: protect from light.

In Vitro

Protocol
1.Protein Preparetion
1) In order to obtain the best labeling effect, please prepare the protein (antibody) concentration as 2 mg/mL.
2) The pH value of protein solution shall be 8.5±0.5. If the pH is lower than 8.0, 1 M sodium bicarbonate shall be used for adjustment.
3) If the protein concentration is lower than 2 mg/mL, the labeling efficiency will be greatly reduced. In order to obtain the best labeling efficiency, it is recommended that the final protein concentration range is 2-10 mg/mL.
4) The protein must be in the buffer without primary amine (such as Tris or glycine) and ammonium ion, otherwise the labeling efficiency will be affected.
2.Dye Preparation (Cy5.5-SE)
Add anhydrous DMSO into the vial of Cy5.5-SE to make a 10 mM stock solution. Mix well by pipetting or vortex.
3.Calculation of dye dosage
The amount of Cy5.5-SE required for reaction depends on the amount of protein to be labeled, and the optimal molar ratio of Cy5.5-SE to protein is about 10.
Example: assuming the required marker protein is 500 μL 2 mg/mL IgG (MW=150,000), use 100 μL DMSO dissolve 1 mg Cy5.5-SE, the required Cy5.5-SE volume is 5.05 μL, and the detailed calculation process is as follows:
1) mmol (IgG) = mg/mL (IgG) ×mL (IgG) / MW (IgG) =2 mg/mL × 0.5 mL / 150,000 mg/mmol= 6.7×10-6 mmol
2) mmol (Cy5.5-SE) = mmol (IgG) × 10 = 6.7×10-6 mmol×10 = 6.7 × 10-5 mmol
3) μL (Cy5.5-SE) = mmol (Cy5.5-SE) ×MW (Cy5.5-SE) / mg/μL (Cy5.5-SE) = 6.7 ×10-5 mmol ×753.88 mg/mmol / 0.01 mg/μL = 5.05 μL (Cy5.5-SE)
4.Run conjugation reaction
1) A good volume of freshly prepared 10 mg/mL Cy5.5-SE is slowly added to 0.5 mL protein sample
In solution, gently shake to mix, then centrifuge briefly to collect the sample at the bottom of the reaction tube. Don'tmix well to prevent protein samples from denaturation and inactivation.
2) The reaction tubules were placed in a dark place and incubated gently at room temperature for 60 minutes at intervals.For 10-15 minutes, gently reverse the reaction tubules several times to fully mix the two reactants and raise the bar efficiency.
5.Purify the conjugation
The following protocol is an example of dye-protein conjugate purification by using a SepHadex G-25 column.
1) Prepare SepHadex G-25 column according to the manufacture instruction.
2) Load the reaction mixture (From "Run conjugation reaction") to the top of the SepHadex G-25 column.
3) Add PBS (pH 7.2-7.4) as soon as the sample runs just below the top resin surface.
4) Add more PBS (pH 7.2-7.4) to the desired sample to complete the column purification. Combine the fractions that contain the desired dye-protein conjugate.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Cy5.5-labeled factor VIIa is developed for imagining cancer. Cy5.5 labeled with these targeting proteins specifically localize to the tumor xenografts for at least 14 days but unconjugated Cy5.5 does not localize to any xenografts or organs. This method of imaging anti-tissue factor in the tumor VECs may be useful in detecting primary tumors and metastases as well as monitoring in vivo therapeutic responses[1]. pH/temperature sensitive magnetic nanogels conjugated with Cy5.5-labled lactoferrin (Cy5.5-Lf-MPNA nanogels) are developed as a promising contrast agent for preoperative MRI and intraoperative fluorescence imaging of glioma[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

1115.32

Formula

C51H62N4O16S4

Appearance

Solid

Color

Blue to dark blue

SMILES

O=S(C1=CC(S(=O)([O-])=O)=C2C(C3=C([N+](CC)=C(/C=C/C=C/C=C4N(CCCCCC(ON5C(CCC5=O)=O)=O)C6=C(C7=CC(S(=O)(O)=O)=CC(S(=O)(O)=O)=C7C=C6)C/4(C)C)C3(C)C)C=C2)=C1)(O)=O.CCN(CC)CC

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, protect from light, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

Solvent & Solubility
In Vitro: 

DMSO : 125 mg/mL (112.08 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 0.8966 mL 4.4830 mL 8.9660 mL
5 mM 0.1793 mL 0.8966 mL 1.7932 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 0.8966 mL 4.4830 mL 8.9660 mL 22.4151 mL
5 mM 0.1793 mL 0.8966 mL 1.7932 mL 4.4830 mL
10 mM 0.0897 mL 0.4483 mL 0.8966 mL 2.2415 mL
15 mM 0.0598 mL 0.2989 mL 0.5977 mL 1.4943 mL
20 mM 0.0448 mL 0.2242 mL 0.4483 mL 1.1208 mL
25 mM 0.0359 mL 0.1793 mL 0.3586 mL 0.8966 mL
30 mM 0.0299 mL 0.1494 mL 0.2989 mL 0.7472 mL
40 mM 0.0224 mL 0.1121 mL 0.2242 mL 0.5604 mL
50 mM 0.0179 mL 0.0897 mL 0.1793 mL 0.4483 mL
60 mM 0.0149 mL 0.0747 mL 0.1494 mL 0.3736 mL
80 mM 0.0112 mL 0.0560 mL 0.1121 mL 0.2802 mL
100 mM 0.0090 mL 0.0448 mL 0.0897 mL 0.2242 mL
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Product Name:
Cy5.5-SE TEA
Cat. No.:
HY-D0925B
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