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  4. Cy5-DBCO

Cy5-DBCO  (Synonyms: DBCO-Sulfo-Cy5)

Cat. No.: HY-D1068 Purity: 98.93%
COA Handling Instructions

Molarity Calculator

Cy5-DBCO (DBCO-Sulfo-Cy5) is a near-infrared (NIR)red fluorescent dye with λabsand λem of 646nm and 670 nm, respectively. Cy5-DBCO (DBCO-Sulfo-Cy5) is not suitable for staining intracellular components of permeabilezed cell, it may exhibits a high background. Cy5-DBCO is a click chemistry reagent, it contains a DBCO group that can undergo strain-promoted alkyne-azide cycloaddition (SPAAC) with molecules containing Azide groups.

For research use only. We do not sell to patients.

Cy5-DBCO Chemical Structure

Cy5-DBCO Chemical Structure

CAS No. : 1564286-24-3

Size Price Stock Quantity
1 mg USD 150 In-stock
5 mg USD 480 In-stock
10 mg USD 770 In-stock
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Based on 1 publication(s) in Google Scholar

Cy5-DBCO Related Antibodies

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Description

Cy5-DBCO (DBCO-Sulfo-Cy5) is a near-infrared (NIR)red fluorescent dye with λabsand λem of 646nm and 670 nm, respectively. Cy5-DBCO (DBCO-Sulfo-Cy5) is not suitable for staining intracellular components of permeabilezed cell, it may exhibits a high background. Cy5-DBCO is a click chemistry reagent, it contains a DBCO group that can undergo strain-promoted alkyne-azide cycloaddition (SPAAC) with molecules containing Azide groups.

IC50 & Target

Red fluorescent dye
In Vitro

Cy5-DBCO (DBCO-Sulfo-Cy5) reacts with azides via a copper-free “click chemistry” reaction and forms a stable triazole and does not need Cu-catalyst or elevated temperatures.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cy5-DBCO Related Antibodies
Molecular Weight

1009.22

Formula

C52H56N4O11S3
CAS No.
Appearance

Solid

Color

Blue to black

Emission (Em)

668
Excitation (Ex)

647
SMILES

O=S(CCC[N+]1=C(/C=C/C=C/C=C(N(CCCCCC(NCCC(N2C3=CC=CC=C3C#CC4=CC=CC=C4C2)=O)=O)C5=CC=C(C=C65)S(=O)(O)=O)/C6(C)C)C(C)(C)C7=CC(S(=O)(O)=O)=CC=C17)([O-])=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

-20°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 62.5 mg/mL (61.93 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H2O : ≥ 10 mg/mL (9.91 mM)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 0.9909 mL 4.9543 mL 9.9086 mL
5 mM 0.1982 mL 0.9909 mL 1.9817 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation

Purity: 99.86%

COA (264 KB) RP-HPLC (598 KB)

Handling Instructions (2659 KB)

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O / DMSO 1 mM 0.9909 mL 4.9543 mL 9.9086 mL 24.7716 mL
5 mM 0.1982 mL 0.9909 mL 1.9817 mL 4.9543 mL
DMSO 10 mM 0.0991 mL 0.4954 mL 0.9909 mL 2.4772 mL
15 mM 0.0661 mL 0.3303 mL 0.6606 mL 1.6514 mL
20 mM 0.0495 mL 0.2477 mL 0.4954 mL 1.2386 mL
25 mM 0.0396 mL 0.1982 mL 0.3963 mL 0.9909 mL
30 mM 0.0330 mL 0.1651 mL 0.3303 mL 0.8257 mL
40 mM 0.0248 mL 0.1239 mL 0.2477 mL 0.6193 mL
50 mM 0.0198 mL 0.0991 mL 0.1982 mL 0.4954 mL
60 mM 0.0165 mL 0.0826 mL 0.1651 mL 0.4129 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Cy5-DBCO Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Cy5-DBCO1564286-24-3DBCO-Sulfo-Cy5Fluorescent DyeFluorescentDyeredcopperInhibitorinhibitorinhibit

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