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  4. CypHer 5

CypHer 5 is a pH-sensitive cyanine derivative and a environmental sensitive fluorophore. CypHer 5 has a pH-sensitive maximal absorption at 644 nm and emission at 664 nm. CypHer 5 has been used as a pH sensor of lysosomal compartments.

For research use only. We do not sell to patients.

CypHer 5 Chemical Structure

CypHer 5 Chemical Structure

CAS No. : 312961-83-4

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5 mg USD 233 In-stock
10 mg USD 350 In-stock
25 mg USD 683 In-stock
50 mg USD 1025 In-stock
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Based on 1 publication(s) in Google Scholar

CypHer 5 Related Antibodies

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Description

CypHer 5 is a pH-sensitive cyanine derivative and a environmental sensitive fluorophore. CypHer 5 has a pH-sensitive maximal absorption at 644 nm and emission at 664 nm. CypHer 5 has been used as a pH sensor of lysosomal compartments[1].

Molecular Weight

628.76

Formula

C31H36N2O8S2
CAS No.
Appearance

Solid

Color

Brown to reddish brown

SMILES

O=C(CCCCCN1C2=CC=C(S(=O)(O)=O)C=C2C(C)(C)/C1=C\C=C\C=C\C3=NC4=C(C3(C)C)C=C(S(=O)(O)=O)C=C4)O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (159.04 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.5904 mL 7.9522 mL 15.9043 mL
5 mM 0.3181 mL 1.5904 mL 3.1809 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo Dissolution Calculator
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Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.5904 mL 7.9522 mL 15.9043 mL 39.7608 mL
5 mM 0.3181 mL 1.5904 mL 3.1809 mL 7.9522 mL
10 mM 0.1590 mL 0.7952 mL 1.5904 mL 3.9761 mL
15 mM 0.1060 mL 0.5301 mL 1.0603 mL 2.6507 mL
20 mM 0.0795 mL 0.3976 mL 0.7952 mL 1.9880 mL
25 mM 0.0636 mL 0.3181 mL 0.6362 mL 1.5904 mL
30 mM 0.0530 mL 0.2651 mL 0.5301 mL 1.3254 mL
40 mM 0.0398 mL 0.1988 mL 0.3976 mL 0.9940 mL
50 mM 0.0318 mL 0.1590 mL 0.3181 mL 0.7952 mL
60 mM 0.0265 mL 0.1325 mL 0.2651 mL 0.6627 mL
80 mM 0.0199 mL 0.0994 mL 0.1988 mL 0.4970 mL
100 mM 0.0159 mL 0.0795 mL 0.1590 mL 0.3976 mL
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CypHer 5 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

CypHer 5312961-83-4CypHer5CypHer-5Fluorescent DyepH-sensitiveenvironmental sensitivecyaninepH sensorlysosomal compartmentsInhibitorinhibitorinhibit

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