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  5. DABCYL-LPETG-EDANS

DABCYL-LPETG-EDANS is a biological active peptide. (This 5-amino acid peptide is a sortase substrate, C-terminal sorting signal. Sortase cleaves surface proteins at the LPXTG motif and catalyzes the formation of an amide bond between the carboxyl group of threonine and the amino group of cell-wall crossbridges. Sortases are a family of Gram-positive transpeptidases responsible for anchoring surface protein virulence factors to the peptidoglycan cell wall layer. Cleavage of this FRET substrate by sortase reveals the fluorescent signal, Abs/Em = 340/490 nm.)

For research use only. We do not sell to patients.

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DABCYL-LPETG-EDANS Chemical Structure

DABCYL-LPETG-EDANS Chemical Structure

CAS No. : 1807943-24-3

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Based on 1 publication(s) in Google Scholar

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Description

DABCYL-LPETG-EDANS is a biological active peptide. (This 5-amino acid peptide is a sortase substrate, C-terminal sorting signal. Sortase cleaves surface proteins at the LPXTG motif and catalyzes the formation of an amide bond between the carboxyl group of threonine and the amino group of cell-wall crossbridges. Sortases are a family of Gram-positive transpeptidases responsible for anchoring surface protein virulence factors to the peptidoglycan cell wall layer. Cleavage of this FRET substrate by sortase reveals the fluorescent signal, Abs/Em = 340/490 nm.)

Molecular Weight

1015.14

Formula

C49H62N10O12S

CAS No.
Appearance

Solid

Color

Brown to reddish brown

Sequence

{Dabcyl}-Leu-Pro-Glu-Thr-Gly-{Edans}

Sequence Shortening

{Dabcyl}-LPETG-{Edans}

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Sealed storage, away from moisture

Powder -80°C 2 years
-20°C 1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (98.51 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 0.9851 mL 4.9254 mL 9.8509 mL
5 mM 0.1970 mL 0.9851 mL 1.9702 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo Dissolution Calculator
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Calculation results:
Working solution concentration: mg/mL
Purity & Documentation

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 0.9851 mL 4.9254 mL 9.8509 mL 24.6271 mL
5 mM 0.1970 mL 0.9851 mL 1.9702 mL 4.9254 mL
10 mM 0.0985 mL 0.4925 mL 0.9851 mL 2.4627 mL
15 mM 0.0657 mL 0.3284 mL 0.6567 mL 1.6418 mL
20 mM 0.0493 mL 0.2463 mL 0.4925 mL 1.2314 mL
25 mM 0.0394 mL 0.1970 mL 0.3940 mL 0.9851 mL
30 mM 0.0328 mL 0.1642 mL 0.3284 mL 0.8209 mL
40 mM 0.0246 mL 0.1231 mL 0.2463 mL 0.6157 mL
50 mM 0.0197 mL 0.0985 mL 0.1970 mL 0.4925 mL
60 mM 0.0164 mL 0.0821 mL 0.1642 mL 0.4105 mL
80 mM 0.0123 mL 0.0616 mL 0.1231 mL 0.3078 mL
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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Product Name:
DABCYL-LPETG-EDANS
Cat. No.:
HY-P5481
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