Demethylcantharidate disodium

Name: Demethylcantharidate disodium
Brand: MedChemExpress
SKU: HY-N1443
Rating: 4.5 (1 reviews)

Cat. No.: HY-N1443 Purity: ≥98.0%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Demethylcantharidate disodium, an endogenous metabolite, induces apoptosis in hepatocellular carcinoma cells via ER stress. Demethylcantharidate disodium shows excellent anticancer activity against multiple types of cancer.

For research use only. We do not sell to patients.

Demethylcantharidate disodium Chemical Structure

CAS No. : 129-67-9

Size	Price	Stock	Quantity
5 mg	USD 32	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 50	In-stock	Estimated Time of Arrival: December 31
50 mg		Get quote
100 mg		Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of Demethylcantharidate disodium:

Endothall Get quote
Sodium Demethylcantharidate Get quote

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Biological Activity
Purity & Documentation
References
Customer Review

Description

In Vitro

Demethylcantharidate (0-100 μM; 0, 12, 24, 48 or 72 hours) disodium inhibits HCC cell proliferation^[1].
Demethylcantharidate (0, 9, 18 or 36 μM; 24 hours) disodium dose dependently increases the levels of cleaved caspase-3, cleaved caspase-9, and Bax/Bcl-2^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:	HCC cell lines (SMMC-7721 and Bel-7402)
Concentration:	0-100 μM
Incubation Time:	0, 12, 24, 48 or 72 hours
Result:	Showed anti-proliferative activity in the two HCC cell lines.

Cell Viability Assay^[1]

Cell Line:	HCC cells
Concentration:	0, 9, 18 or 36 µM
Incubation Time:	24 hours
Result:	Induced apoptosis in HCC cells via the intrinsic pathway.

In Vivo

Demethylcantharidate disodium significantly decreases hepatocellular carcinoma tumorigenesis of SMMC-7721 cells in vivo^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Clinical Trial

Molecular Weight

230.13

Formula

C₈H₈Na₂O₅

CAS No.

129-67-9

Appearance

Solid

Color

White to off-white

SMILES

O=C(C1C(C2OC1CC2)C(O[Na])=O)O[Na]

Structure Classification

Ketones, Aldehydes, Acids

Initial Source

Endogenous metabolite

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility

In Vitro:

H₂O : 33.33 mg/mL (144.83 mM; ultrasonic and warming and heat to 60°C)

DMSO : < 1 mg/mL (insoluble or slightly soluble)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	4.3454 mL	21.7269 mL	43.4537 mL
5 mM	0.8691 mL	4.3454 mL	8.6907 mL
10 mM	0.4345 mL	2.1727 mL	4.3454 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 50 mg/mL (217.27 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: ≥98.0%

Select Batch:

Data Sheet (280 KB) SDS (252 KB)

COA (259 KB) HNMR (229 KB) MS (195 KB)

Handling Instructions (2659 KB)

References

[1]. Ye M, et al. Sodium demethylcantharidate induces apoptosis in hepatocellular carcinoma cells via ER stress. Am J Transl Res. 2019;11(5):3150-3158. Published 2019 May 15. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O	1 mM	4.3454 mL	21.7268 mL	43.4537 mL	108.6342 mL
	5 mM	0.8691 mL	4.3454 mL	8.6907 mL	21.7268 mL
	10 mM	0.4345 mL	2.1727 mL	4.3454 mL	10.8634 mL
	15 mM	0.2897 mL	1.4485 mL	2.8969 mL	7.2423 mL
	20 mM	0.2173 mL	1.0863 mL	2.1727 mL	5.4317 mL
	25 mM	0.1738 mL	0.8691 mL	1.7381 mL	4.3454 mL
	30 mM	0.1448 mL	0.7242 mL	1.4485 mL	3.6211 mL
	40 mM	0.1086 mL	0.5432 mL	1.0863 mL	2.7159 mL
	50 mM	0.0869 mL	0.4345 mL	0.8691 mL	2.1727 mL
	60 mM	0.0724 mL	0.3621 mL	0.7242 mL	1.8106 mL
	80 mM	0.0543 mL	0.2716 mL	0.5432 mL	1.3579 mL
	100 mM	0.0435 mL	0.2173 mL	0.4345 mL	1.0863 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.