Diethyl 2,4,6-trimethyl-1,4-dihydropyridine-3,5-dicarboxylate

Cat. No.: HY-118594 Purity: 99.79%: FAQs
Data Sheet
SDS

COA
Handling Instructions Technical Support

Diethyl 2,4,6-trimethyl-1,4-dihydropyridine-3,5-dicarboxylate is a ferrochelatase inhibitor that blocks heme synthesis. Diethyl 2,4,6-trimethyl-1,4-dihydropyridine-3,5-dicarboxylate induces Mallory Denk bodies (MDB) formation. Diethyl 2,4,6-trimethyl-1,4-dihydropyridine-3,5-dicarboxylate can be used in the fields of iron metabolism, oxidative stress, and liver-related diseases.

For research use only. We do not sell to patients.

Diethyl 2,4,6-trimethyl-1,4-dihydropyridine-3,5-dicarboxylate Chemical Structure

CAS No. : 632-93-9

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Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
500 mg	In-stock	Estimated Time of Arrival: December 31
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Purity & Documentation
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Description

In Vivo

Diethyl 2,4,6-trimethyl-1,4-dihydropyridine-3,5-dicarboxylate (0.17%; fed; continuously for 10 weeks) in 1-month-old male C₃H mice induces the formation of Mallory Denk bodies (MDB). Refeeding Diethyl 2,4,6-trimethyl-1,4-dihydropyridine-3,5-dicarboxylate after drug withdrawal increases the proportion of FAT10-positive hepatocytes. Diethyl 2,4,6-trimethyl-1,4-dihydropyridine-3,5-dicarboxylate also alters the expression of tumor-related genes, and liver tumors form in mice after long-term drug withdrawal^[1].
Diethyl 1,4-dihydro-2,4,6-trimethyl-3,5-pyridinedicarboxylate (20 mg/100 g body weight; intraperitoneal injection; once, 4 h before Phenobarbital, Phorone, or Hemin (HY-19424) administration) blocks heme synthesis, prevents the induction of HO-1 by oxidative stress, and inhibits the increase in serum iron induced by phenobarbital in C57BL/6 mice with selenium deficiency or sufficiency^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	1-month-old male C₃H mice^[1].
Dosage:	0.17%
Administration:	Fed continuously for 10 weeks
Result:	Induced MDB formation in the livers of mice.

Animal Model:	Male weanling C57BL/6 mice were fed with selenium-deficient diet (less than 0.02 mg selenium/kg) or selenium-replete (control) diet (supplemented with 0.25 mg selenium/kg as sodium selenite)^[2].
Dosage:	20 mg/100 g body weight
Administration:	Administered intraperitoneally once, 4 h before phenobarbital, phorone, or Hemin (HY-19424) administration
Result:	Blocked the induction of HO activity and HO-1 protein by phenobarbital in selenium-deficient mice. Inhibited the rise in serum iron induced by phenobarbital. Inhibited the induction of HO-1 by phorone treatment in selenium-replete mice. Did not block the induction of HO-1 by hemin in either selenium - deficient or selenium-replete mice.

Molecular Weight

267.32

Formula

C₁₄H₂₁NO₄

CAS No.

632-93-9

Appearance

Solid

Color

White to off-white

SMILES

CC1C(C(OCC)=O)=C(NC(C)=C1C(OCC)=O)C

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (374.08 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.7408 mL	18.7042 mL	37.4083 mL
5 mM	0.7482 mL	3.7408 mL	7.4817 mL
10 mM	0.3741 mL	1.8704 mL	3.7408 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (9.35 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.79%

Select Batch:

Data Sheet (272 KB) SDS (393 KB)

COA (245 KB) RP-HPLC (219 KB)

Handling Instructions (2659 KB)

References

[1]. Oliva J, et al. Fat10 is an epigenetic marker for liver preneoplasia in a drug-primed mouse model of tumorigenesis. Exp Mol Pathol. 2008 Apr;84(2):102-12. [Content Brief]

[2]. Mostert V, et al. Serum iron increases with acute induction of hepatic heme oxygenase-1 in mice. Drug Metab Rev. 2007;39(2-3):619-26. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	3.7408 mL	18.7042 mL	37.4083 mL	93.5209 mL
	5 mM	0.7482 mL	3.7408 mL	7.4817 mL	18.7042 mL
	10 mM	0.3741 mL	1.8704 mL	3.7408 mL	9.3521 mL
	15 mM	0.2494 mL	1.2469 mL	2.4939 mL	6.2347 mL
	20 mM	0.1870 mL	0.9352 mL	1.8704 mL	4.6760 mL
	25 mM	0.1496 mL	0.7482 mL	1.4963 mL	3.7408 mL
	30 mM	0.1247 mL	0.6235 mL	1.2469 mL	3.1174 mL
	40 mM	0.0935 mL	0.4676 mL	0.9352 mL	2.3380 mL
	50 mM	0.0748 mL	0.3741 mL	0.7482 mL	1.8704 mL
	60 mM	0.0623 mL	0.3117 mL	0.6235 mL	1.5587 mL
	80 mM	0.0468 mL	0.2338 mL	0.4676 mL	1.1690 mL
	100 mM	0.0374 mL	0.1870 mL	0.3741 mL	0.9352 mL