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  4. Diethyl succinate

Diethyl succinate  (Synonyms: Diethyl Butanedioate)

Cat. No.: HY-Y0836 Purity: ≥98.0%
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Diethyl succinate (Diethyl Butanedioate) can be utilized at physiological pH, allowing it to penetrate biological membranes and integrate into the cells of tissue cultures, where it is metabolized via the tricarboxylic acid cycle. Diethyl succinate modulates the polarization and activation of microglial cells by reducing mitochondrial fission and the levels of reactive oxygen species (ROS), thereby exerting an inflammatory protective effect in primary microglial cells. Furthermore, Diethyl succinate is non-toxic and can be used in flavorings and seasonings.

For research use only. We do not sell to patients.

Diethyl succinate Chemical Structure

Diethyl succinate Chemical Structure

CAS No. : 123-25-1

Size Price Stock Quantity
Liquid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 61 In-stock
Solution
10 mM * 1 mL in DMSO USD 61 In-stock
Liquid
100 mg USD 55 In-stock
250 mg USD 110 In-stock
500 mg USD 190 In-stock
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Based on 1 publication(s) in Google Scholar

Other Forms of Diethyl succinate:

Diethyl succinate Related Antibodies

Top Publications Citing Use of Products

1 Publications Citing Use of MCE Diethyl succinate

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Diethyl succinate (Diethyl Butanedioate) can be utilized at physiological pH, allowing it to penetrate biological membranes and integrate into the cells of tissue cultures, where it is metabolized via the tricarboxylic acid cycle. Diethyl succinate modulates the polarization and activation of microglial cells by reducing mitochondrial fission and the levels of reactive oxygen species (ROS), thereby exerting an inflammatory protective effect in primary microglial cells. Furthermore, Diethyl succinate is non-toxic and can be used in flavorings and seasonings[1][2].

In Vitro

Diethyl succinate (5 mM; 3 h) reduces ROS production in primary microglia[2].
Diethyl succinate (0-10 mM; 0.25-3 h) induces decreased mitochondrial fission in primary microglia[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Diethyl succinate Related Antibodies

Western Blot Analysis[2]

Cell Line: Primary microglia
Concentration: 0, 0.2, 0.5, 1, 5 and 10 mM
Incubation Time: 0.25, 1 and 3 h
Result: Increased phosphorylation of DRP1 at S637.
Molecular Weight

174.19

Formula

C8H14O4
CAS No.
Appearance

Liquid (Density: 1.047 g/cm3)

Color

Colorless to light yellow

SMILES

O=C(OCC)CCC(OCC)=O
Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Pure form -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : ≥ 250 mg/mL (1435.21 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 5.7409 mL 28.7043 mL 57.4086 mL
5 mM 1.1482 mL 5.7409 mL 11.4817 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.08 mg/mL (11.94 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 5.7409 mL 28.7043 mL 57.4086 mL 143.5214 mL
5 mM 1.1482 mL 5.7409 mL 11.4817 mL 28.7043 mL
10 mM 0.5741 mL 2.8704 mL 5.7409 mL 14.3521 mL
15 mM 0.3827 mL 1.9136 mL 3.8272 mL 9.5681 mL
20 mM 0.2870 mL 1.4352 mL 2.8704 mL 7.1761 mL
25 mM 0.2296 mL 1.1482 mL 2.2963 mL 5.7409 mL
30 mM 0.1914 mL 0.9568 mL 1.9136 mL 4.7840 mL
40 mM 0.1435 mL 0.7176 mL 1.4352 mL 3.5880 mL
50 mM 0.1148 mL 0.5741 mL 1.1482 mL 2.8704 mL
60 mM 0.0957 mL 0.4784 mL 0.9568 mL 2.3920 mL
80 mM 0.0718 mL 0.3588 mL 0.7176 mL 1.7940 mL
100 mM 0.0574 mL 0.2870 mL 0.5741 mL 1.4352 mL
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Diethyl succinate Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Diethyl123-25-1Diethyl ButanedioateBiochemical Assay ReagentsReactive Oxygen SpeciesInhibitorinhibitorinhibit

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