2. Immunology/Inflammation Apoptosis
  3. CD47 Apoptosis
  4. DMUP

DMUP is a potent CD47-SIRPα axis inhibitor. DMUP induces apoptosis and increases the macrophage phagocytosis in A549 cells. DMUP decreases the expression of CD47 and SIRPα protein. DMUP shows antitumor activity.

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DMUP Chemical Structure

DMUP Chemical Structure

CAS No. : 2364350-07-0

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10 mg USD 1199 In-stock
25 mg USD 2518 In-stock
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Based on 1 publication(s) in Google Scholar

DMUP Related Antibodies

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Description

DMUP is a potent CD47-SIRPα axis inhibitor. DMUP induces apoptosis and increases the macrophage phagocytosis in A549 cells. DMUP decreases the expression of CD47 and SIRPα protein. DMUP shows antitumor activity[1].

In Vitro

DMUP (72 h) shows antiproliferative activity with IC50s of 0.92, 3.58, 6.29, 1.54 μM for A549, A549/DDP, PANC-1, HepG2 cells, respectively[1].
DMUP (5 μM, 24 h) arrests the cell cycle in S phase in A549 cells[1].
DMUP (5 μM, 48 h) induces apoptosis in A549 cells[1].
DMUP (5 μM, 24 h) decreases the expression of CD47 and SIRPα protein[1].
DMUP (5 μM, 4 h) increases the macrophage phagocytosis in A549-GFP cells[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

DMUP Related Antibodies

Cell Proliferation Assay[1]

Cell Line: A549, A549/DDP, PANC-1, HepG2 cells
Concentration: 0-64 µM
Incubation Time: 72 h
Result: Showed antiproliferative activity with IC50s of 0.92, 3.58, 6.29, 1.54 µM for A549, A549/DDP, PANC-1, HepG2 cells, respectively.

Cell Cycle Analysis[1]

Cell Line: A549 cells
Concentration: 5 µM
Incubation Time: 24 h
Result: Arrested the cell cycle in the S phase.

Apoptosis Analysis[1]

Cell Line: A549 cells
Concentration: 5 µM
Incubation Time: 48 h
Result: Induced cell apoptosis.

Western Blot Analysis[1]

Cell Line: A549, THP-1 cells
Concentration: 5 µM
Incubation Time: 24 h
Result: Decreased the expression of CD47 and SIRPα protein.
In Vivo

DMUP (10 mg/kg, i.v., every other day for 17 day) shows antitumor activity[1].
DMUP (5, 10, 20, 40 mg/kg, i.v.,every two days for 18 days) shows no toxicity in mice[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: ICR mice[1]
Dosage: 5, 10, 20, 40 mg/kg
Administration: i.v., every two days, 18 days
Result: Showed low toxicity with LD50 of greater than 20 mg/kg.
Animal Model: A549 xenograft BALB/c nude mice[1]
Dosage: 10 mg/kg
Administration: i.v., every other day, 17 day
Result: Showed antitumor activity.
Molecular Weight

766.44

Formula

C24H24Cl2N2O10Pt
CAS No.
Appearance

Solid

Color

White to light yellow

SMILES

O=C1OC2=C(C(C)=C1)C=CC(OCC([O-][Pt+4]([Cl-])([O-]C(COC3=CC(OC(C=C4C)=O)=C4C=C3)=O)([NH3])([NH3])[Cl-])=O)=C2
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (130.47 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.3047 mL 6.5237 mL 13.0473 mL
5 mM 0.2609 mL 1.3047 mL 2.6095 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    90% Corn Oil

    Solubility: ≥ 2.5 mg/mL (3.26 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
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% 
+
%
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%
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Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.3047 mL 6.5237 mL 13.0473 mL 32.6183 mL
5 mM 0.2609 mL 1.3047 mL 2.6095 mL 6.5237 mL
10 mM 0.1305 mL 0.6524 mL 1.3047 mL 3.2618 mL
15 mM 0.0870 mL 0.4349 mL 0.8698 mL 2.1746 mL
20 mM 0.0652 mL 0.3262 mL 0.6524 mL 1.6309 mL
25 mM 0.0522 mL 0.2609 mL 0.5219 mL 1.3047 mL
30 mM 0.0435 mL 0.2175 mL 0.4349 mL 1.0873 mL
40 mM 0.0326 mL 0.1631 mL 0.3262 mL 0.8155 mL
50 mM 0.0261 mL 0.1305 mL 0.2609 mL 0.6524 mL
60 mM 0.0217 mL 0.1087 mL 0.2175 mL 0.5436 mL
80 mM 0.0163 mL 0.0815 mL 0.1631 mL 0.4077 mL
100 mM 0.0130 mL 0.0652 mL 0.1305 mL 0.3262 mL
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DMUP Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

DMUP2364350-07-0CD47ApoptosisCluster of Differentiation 47MER6OA3Platinum(IV)peripheral blood mononuclear cells (PBMCs)macrophage phagocytosisA549 cellsS phaseapoptosisantitumorInhibitorinhibitorinhibit

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