2. Epigenetics
  3. DNA Methyltransferase
  4. DNMT3A-IN-1

DNMT3A-IN-1 (compound 1) is an effective and selective DNMT3A inhibitor. DNMT3A-IN-1 exhibits inhibitory activity against DNMT3A, with KI values ranging from 9.16 to 18.85 μM (AdoMet) and 11.37 to 23.34 μM (poly dI-dC). DNMT3A-IN-1 can induce apoptosis in acute myeloid leukemia (AML) cell lines (Apoptosis).

For research use only. We do not sell to patients.

DNMT3A-IN-1 Chemical Structure

DNMT3A-IN-1 Chemical Structure

CAS No. : 1403598-56-0

Size Price Stock Quantity
1 mg USD 300 In-stock
5 mg USD 750 In-stock
10 mg USD 1180 In-stock
25 mg USD 2300 In-stock
50 mg USD 3800 In-stock
100 mg USD 6200 In-stock
200 mg   Get quote  
500 mg   Get quote  

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 1 publication(s) in Google Scholar

DNMT3A-IN-1 Related Antibodies

Top Publications Citing Use of Products

1 Publications Citing Use of MCE DNMT3A-IN-1

View All DNA Methyltransferase Isoform Specific Products:

View All Isoforms
DNA Methyltransferase DNMT1 DNMT3 MGMT

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

DNMT3A-IN-1 (compound 1) is an effective and selective DNMT3A inhibitor. DNMT3A-IN-1 exhibits inhibitory activity against DNMT3A, with KI values ranging from 9.16 to 18.85 μM (AdoMet) and 11.37 to 23.34 μM (poly dI-dC). DNMT3A-IN-1 can induce apoptosis in acute myeloid leukemia (AML) cell lines (Apoptosis)[1][2].

IC50 & Target

DNMT3A

 

Cellular Effect
Cell Line Type Value Description References
HeLa IC50
> 25 μM
Compound: 25; MMV690028
Cytotoxicity against human HeLa cells assessed as reduction in cell viability after 48 hrs by resazurin dye based fluorescence assay
Cytotoxicity against human HeLa cells assessed as reduction in cell viability after 48 hrs by resazurin dye based fluorescence assay
[PMID: 30647879]
MRC5 CC50
> 64 μM
Compound: 20b, VUF13525
Cytotoxicity against human MRC5 cells after 72 hrs by resazurin assay
Cytotoxicity against human MRC5 cells after 72 hrs by resazurin assay
[PMID: 22963052]
In Vitro

DNMT3A-IN-1 (6 µM and 60 µM, 30 minutes) effectively inhibits the methylation activity of recombinant DNMT3A protein[1].
DNMT3A-IN-1 (5–12 μM, 72 hours) significantly induces apoptosis in acute myeloid leukemia (AML) cell lines[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

DNMT3A-IN-1 Related Antibodies

Apoptosis Analysis[2]

Cell Line: MV411, MOLM13, THP-1, OCI-AML3, KASUMI, HL60 cells
Concentration: 5–12 μM
Incubation Time: 72 h
Result: Significantly induced apoptosis and upregulated CD11b differentiation marker at 5–12 μM concentrations in AML cells.
Molecular Weight

546.66

Formula

C30H38N6O4
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O=C1N(C2CCCCCC2)N=C(C1(C)C)C3=CC(OCCCCOC4=CC=C(C5=NN=NN5)C=C4)=C(OC)C=C3
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (182.93 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.8293 mL 9.1465 mL 18.2929 mL
5 mM 0.3659 mL 1.8293 mL 3.6586 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.8293 mL 9.1465 mL 18.2929 mL 45.7323 mL
5 mM 0.3659 mL 1.8293 mL 3.6586 mL 9.1465 mL
10 mM 0.1829 mL 0.9146 mL 1.8293 mL 4.5732 mL
15 mM 0.1220 mL 0.6098 mL 1.2195 mL 3.0488 mL
20 mM 0.0915 mL 0.4573 mL 0.9146 mL 2.2866 mL
25 mM 0.0732 mL 0.3659 mL 0.7317 mL 1.8293 mL
30 mM 0.0610 mL 0.3049 mL 0.6098 mL 1.5244 mL
40 mM 0.0457 mL 0.2287 mL 0.4573 mL 1.1433 mL
50 mM 0.0366 mL 0.1829 mL 0.3659 mL 0.9146 mL
60 mM 0.0305 mL 0.1524 mL 0.3049 mL 0.7622 mL
80 mM 0.0229 mL 0.1143 mL 0.2287 mL 0.5717 mL
100 mM 0.0183 mL 0.0915 mL 0.1829 mL 0.4573 mL
  • No file chosen (Maximum size is: 1024 Kb)
  • If you have published this work, please enter the PubMed ID.
  • Your name will appear on the site.

DNMT3A-IN-1 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

DNMT3A-IN-11403598-56-0DNA MethyltransferaseDNMTsDNA MTasesMethyltransferasesPyridazineselectiveAcute myeloid leukemia targetNon-nucleosidede novo DNA methylationInhibitorinhibitorinhibit

Your Recently Viewed Products:

Inquiry Online

Your information is safe with us. * Required Fields.

Product Name

  

Salutation

Applicant Name *

 

Email Address *

Phone Number *

 

Organization Name *

Department *

 

Requested quantity *

Country or Region *

      

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
DNMT3A-IN-1
Cat. No.:
HY-144433
Quantity:
MCE Japan Authorized Agent:

Customer Review

Thank You for Your Feedback!

Request for HNMR Report

Please fill out this form to request the QC report. We will send it to your Email address shortly.

Your information is safe with us. * Required Fields.

Product Name

  

Lot #

Applicant Name *

 

Email Address *

Phone Number

 

Department *

Organization Name *

 

Country or Region *

Remarks

SAFETY DATA SHEET (SDS)

English - EN (251 KB) Français - FR (251 KB) Deutsch - DE (251 KB) Norwegian - NO (251 KB) Español - ES (251 KB) Swedish - SV (251 KB)

Request for HNMR Report

We have received your request and will respond to you as soon as possible.