Endothelin-1 (1-31) (Human) acetate

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Endothelin-1 (1-31) (Human) acetate is a potent vasoconstrictor and hypertensive agent. Endothelin-1 (1-31) (Human) acetate is derived from the selective hydrolysis of big ET-1 by chymase.

For research use only. We do not sell to patients.

Endothelin-1 (1-31) (Human) acetate Chemical Structure

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Other Forms of Endothelin-1 (1-31) (Human) acetate:

Endothelin-1 (1-31) (Human) Get quote
Endothelin-1 (1-31) (Human) TFA Get quote

1 Publications Citing Use of MCE Endothelin-1 (1-31) (Human) acetate

•Acta Pharmacol Sin. 2024 Mar;45(3):545-557. [Abstract]

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Biological Activity
Purity & Documentation
References
Customer Review

Description

Endothelin-1 (1-31) (Human) acetate is a potent vasoconstrictor and hypertensive agent. Endothelin-1 (1-31) (Human) acetate is derived from the selective hydrolysis of big ET-1 by chymase^[1].

In Vitro

Endothelin-1 (1-31) (Human) (100 pM-100 nM; 24 h) acetate induces human mesangial cells proliferation^[2].
Endothelin-1 (1-31) (Human) (100 nM; 0-10 min) acetate induces ERK activation in human mesangial cells^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Proliferation Assay^[2]

Cell Line:	Human mesangial cells
Concentration:	100 pM-100 nM
Incubation Time:	24 h
Result:	Caused an increase in [³H]-thymidine incorporation into the cells in a concentration-dependent manner.

Western Blot Analysis^[2]

Cell Line:	Human mesangial cells
Concentration:	100 nM
Incubation Time:	0, 5, 10, 15 and 30 min
Result:	ERK activities rapidly increased 2.45-fold at 5 min and peaked at 10 min. The activities of both ERKs rapidly declined, returning to the baseline control value 30 min after stimulation.

In Vivo

ET-1 (1-31) (100 nM; single dose) acetate induces contraction in the mouse mesenteric artery. The contraction may be mediated by the ET_A receptor and may be increased by aging. A clear difference exists between males and females in the present chronic diabetic condition^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	ICR mice, Streptozocin (HY-13753)-induced diabetic model^[1]
Dosage:	100 nM
Administration:	In the organ bath, single dose
Result:	In the 1-week control (but not diabetic) group, induced contraction and the contractile response was significantly greater in female mice than in male mice, and there was no significant difference in either male or female mice between the age-matched controls and the diabetic mice. In the 8-weeks group, the contraction was or tended to be increased compared with the corresponding 1-week group in all mice. Although in male mice this contraction was not different between control and diabetic groups, it was significantly greater in diabetic female mice than in the control female mice and in female diabetic mice than in male diabetic mice. The contraction was inhibited by ET_A receptor inhibitor.

Molecular Weight

3688.21

Formula

C₁₆₄H₂₄₀N₃₈O₄₉S₅

Sequence

Cys-Ser-Cys-Ser-Ser-Leu-Met-Asp-Lys-Glu-Cys-Val-Tyr-Phe-Cys-His-Leu-Asp-Ile-Ile-Trp-Val-Asn-Thr-Pro-Glu-His-Val-Val-Pro-Tyr (Disulfide bridge:Cys1-Cys15;Cys3-Cys11)

Sequence Shortening

CSCSSLMDKECVYFCHLDIIWVNTPEHVVPY (Disulfide bridge:Cys1-Cys15;Cys3-Cys11)

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Solvent & Solubility

In Vitro:

H₂O

Peptide Solubility and Storage Guidelines:

1. Calculate the length of the peptide.

2. Calculate the overall charge of the entire peptide according to the following table:

	Contents	Assign value
Acidic amino acid	Asp (D), Glu (E), and the C-terminal -COOH.	-1
Basic amino acid	Arg (R), Lys (K), His (H), and the N-terminal -NH₂	+1
Neutral amino acid	Gly (G), Ala (A), Leu (L), Ile (I), Val (V), Cys (C), Met (M), Thr (T), Ser (S), Phe (F), Tyr (Y), Trp (W), Pro (P), Asn (N), Gln (Q)	0

3. Recommended solution:

Overall charge of peptide	Details
Negative (<0)	1. Try to dissolve the peptide in water first. 2. If water fails, add NH₄OH (<50 μL). 3. If the peptide still does not dissolve, add DMSO (50-100 μL) to solubilize the peptide.
Positive (>0)	1. Try to dissolve the peptide in water first. 2. If water fails, try dissolving the peptide in a 10%-30% acetic acid solution. 3. If the peptide still does not dissolve, try dissolving the peptide in a small amount of DMSO.
Zero (=0)	1. Try to dissolve the peptide in organic solvent (acetonitrile, methanol, etc.) first. 2. For very hydrophobic peptides, try dissolving the peptide in a small amount of DMSO, and then dilute the solution with water to the desired concentration.

Purity & Documentation

Handling Instructions (2659 KB)

References

[1]. Matsumoto T, et al. Gender differences in vascular reactivity to endothelin-1 (1-31) in mesenteric arteries from diabetic mice. Peptides. 2008 Aug;29(8):1338-46. [Content Brief]

[2]. Yoshizumi M, et al. Effect of endothelin-1 (1-31) on human mesangial cell proliferation. Jpn J Pharmacol. 2000 Oct;84(2):146-55. [Content Brief]