2. Cell Cycle/DNA Damage
  3. DNA Stain
  4. Ethidium monoazide bromide

Ethidium monoazide bromide is a DNA intercalating fluorescent dye that enters bacteria with damaged membranes. Ethidium monoazide bromide can be covalently linked to DNA by photoactivation. Ethidium monoazide bromide stains only dead cells. Ethidium monoazide (bromide) is a click chemistry reagent, it contains an Azide group and can undergo copper-catalyzed azide-alkyne cycloaddition reaction (CuAAc) with molecules containing Alkyne groups. It can also undergo strain-promoted alkyne-azide cycloaddition (SPAAC) reactions with molecules containing DBCO or BCN groups.

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Ethidium monoazide bromide Chemical Structure

Ethidium monoazide bromide Chemical Structure

CAS No. : 58880-05-0

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Description

Ethidium monoazide bromide is a DNA intercalating fluorescent dye that enters bacteria with damaged membranes. Ethidium monoazide bromide can be covalently linked to DNA by photoactivation. Ethidium monoazide bromide stains only dead cells[1]. Ethidium monoazide (bromide) is a click chemistry reagent, it contains an Azide group and can undergo copper-catalyzed azide-alkyne cycloaddition reaction (CuAAc) with molecules containing Alkyne groups. It can also undergo strain-promoted alkyne-azide cycloaddition (SPAAC) reactions with molecules containing DBCO or BCN groups.

Molecular Weight

420.31

Formula

C21H18BrN5
CAS No.
Emission (Em)

600
Excitation (Ex)

504
SMILES

CC[N+]1=C(C2=CC=CC=C2)C3=CC(N=[N+]=[N-])=CC=C3C4=C1C=C(N)C=C4.[Br-]
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Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
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Ethidium monoazide bromide Related Classifications

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  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Ethidium monoazide58880-05-0DNA StainDNA intercalatingdamaged membranesdead cellsInhibitorinhibitorinhibit

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