FITC-Dextran (MW 10000)

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FITC-Dextran (MW 10000) is a fluorescent probe for fluorescein isothiocyanate (FITC) dextran (Ex=495 nm; Em=525 nm). FITC-Dextran (MW 10000) can be used as a marker to reveal heat shock-induced cell damage and to study the early and late stages of apoptosis. FITC-Dextran (MW 10000) can also be used for cell permeability studies, such as blood-brain barrier permeability and determination of the extent of blood-brain barrier disruption.

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FITC-Dextran (MW 10000) Chemical Structure

CAS No. : 60842-46-8

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Based on 47 publication(s) in Google Scholar

Other Forms of FITC-Dextran (MW 10000):

47 Publications Citing Use of MCE FITC-Dextran (MW 10000)

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Description	FITC-Dextran (MW 10000) is a fluorescent probe for fluorescein isothiocyanate (FITC) dextran (Ex=495 nm; Em=525 nm). FITC-Dextran (MW 10000) can be used as a marker to reveal heat shock-induced cell damage and to study the early and late stages of apoptosis. FITC-Dextran (MW 10000) can also be used for cell permeability studies, such as blood-brain barrier permeability and determination of the extent of blood-brain barrier disruption^[1]^[2]^[3].
In Vitro	Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs). Labeling of cells^[1]: For use with apoptotic HeLa cells and human peripheral blood mononuclear cells (PBMC) (viable HeLa and PBMC can not be stained by FITC-Dextran). 1. Incubate cells at 43.5°C for 1 hour and at 37°C for 8 hours to induce apoptosis. 2. Suspend the cells in 100 μL of medium, and mix in Q-prep tubes with 10 μL of propidium iodide (PI), 10 μL of FITC-Dextran (MW 10000) (the final concentration of PI and FITC-Dextran (MW 10000) is 7.5 μM and 1.13 μM, respectively). 3. Incubate cells for 25 min at room temperature in the dark. 4. Take the labeled cells with 3 mL of medium and centrifuge for 10 min at 500 g. 5. Take centrifuged cells with 1 mL of medium and use flow cytometry or fluorescence microscopy analyze (PI: Ex=500 nm, Em=600 nm; FITC-Dextran (MW 10000): Ex=495 nm, Em=525 nm). Paracellular permeability measurement^[4] 1. Add FITC-dextran (0.1 mg/mL) to the basal media in the transwell chamber. 2. Collect media from the transwell insert after 15 min. 3. Measure the fluorescence signal (Ex=485 nm, Em=538 nm). 4. Calculate FITC-dextran concentration based on fluorescence intensity. 5. Calculate permeability. MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. FITC-Dextran (MW 10000) Related Antibodies
In Vivo	Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs). For intestinal barrier function assay^[5] 1. Fast mice for 4 h. 2. Orally gavage mice with FITC-Dextran MW 10000 (0.6 mg/g). 3. Measure fluorescence intensity of plasma in 4 h (excitation nm/emission 520 nm). MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Molecular Weight	10000.00(Average)
CAS No.	60842-46-8
Appearance	Solid
Color	Yellow to brown
Emission (Em)	525
Excitation (Ex)	488
SMILES	[FITC-Dextran (MW 10000)]
Shipping	Room temperature in continental US; may vary elsewhere.
Storage	4°C, protect from light *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Solvent & Solubility	In Vitro: H₂O : 50 mg/mL (Need ultrasonic) Molarity Calculator Dilution Calculator Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol) Mass = Concentration × Volume × Molecular Weight * Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final) This equation is commonly abbreviated as: C₁V₁ = C₂V₂ Concentration _(start) C1 × Volume _(start) V1 = Concentration _(final) C2 × Volume _(final) V2 In Vivo: For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time. The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution. Protocol 1 Add each solvent one by one: PBS Solubility: 100 mg/mL; Clear solution; Need ultrasonic In Vivo Dissolution Calculator Please enter the basic information of animal experiments: Dosage mg/kg Animal weight (per animal) g Dosing volume (per animal) μL Number of animals Recommended: Prepare an additional quantity of animals to account for potential losses during experiments. Calculation results: Working solution concentration: mg/mL This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details. The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE). Tel: 609-228-6898 E-mail: sales@medchemexpress.com Technical Support: tech@medchemexpress.com
Purity & Documentation	Select Batch: Data Sheet (280 KB) SDS (393 KB) COA (254 KB) Handling Instructions (2659 KB)
Dyeing Example	Description: FITC-Dextran MW 10000 can be used to explore the cellular uptake with green fluorescence. Method: For cellular uptake monitoring. 1. Incubate cells in glass dishes (37℃; overnight). 2. Incubate cells with FITC-Dextran MW 10000 (5 μg/mL; 4 h). 3. Wash cells for twice with PBS and finally fix cells with 4% PFA (30 min; dark). 4. Use a confocal laser scanning microscopy (Zeiss 880) for image. Description: FITC-Dextran MW 10000 can be used to detect intestinal barrier function in vivo. Method: For assess intestinal barrier function. 1. Fast mice for 4 hours. 2. Orally gavage mice with FITC-Dextran MW 10000 (0.6 mg/g). 3. Measure fluorescence intensity of plasma in 4 hours. Description: FITC-Dextran MW 10000 can be used for labeling microvessels in the lesion area of rat brain to measure microvessel density. Method: For in vivo labeling. 1. Anesthetize rats first. 2. Inject FITC-Dextran MW 10000 (50 mg/kg) in the tail vein of rats. 3. Remove brain tissues 1 min after injection, use 4% paraformaldehyde (4°C; 24 h) to fix tissues and cut tissues into 150 µm-thick sections. 4. Use a laser scanning confocal microscope (Leica, Solms, Germany) for image.
References	[1]. Moumaris M, et al. Fluorescein isothiocyanate-dextran can track apoptosis and necrosis induced by heat shock of peripheral blood mononuclear cells and HeLa cells[J]. Open Biological Sciences Journal, 2015, 1(1). [2]. Natarajan R, et al. Fluorescein Isothiocyanate (FITC)-Dextran Extravasation as a Measure of Blood-Brain Barrier Permeability. Curr Protoc Neurosci. 2017 Apr 10;79:9.58.1-9.58.15. [Content Brief] [3]. Eriksson I, et al. Analysis of Lysosomal pH by Flow Cytometry Using FITC-Dextran Loaded Cells. Methods Mol Biol. 2017;1594:179-189. [Content Brief] [4]. Okabayashi K, et al. Cdc42 activates paracellular transport in polarised submandibular gland cells. Arch Oral Biol. 2021 Dec;132:105276. [Content Brief] [5]. Yu W, et al. ACE2 contributes to the maintenance of mouse epithelial barrier function. Biochem Biophys Res Commun. 2020 Dec 17;533(4):1276-1282. [Content Brief]