FITC-Dextran (MW 4000)

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FITC-Dextran (MW 4000) is a fluorescent probe for fluorescein isothiocyanate (FITC) dextran (Ex=495 nm; Em=525 nm). FITC-Dextran (MW 4000) can be used as a marker to reveal heat shock-induced cell damage and to study the early and late stages of apoptosis. FITC-Dextran (MW 4000) can also be used for cell permeability studies, such as blood-brain barrier permeability and determination of the extent of blood-brain barrier disruption.

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FITC-Dextran (MW 4000) Chemical Structure

CAS No. : 60842-46-8

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Based on 45 publication(s) in Google Scholar

Other Forms of FITC-Dextran (MW 4000):

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45 Publications Citing Use of MCE FITC-Dextran (MW 4000)

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Description	FITC-Dextran (MW 4000) is a fluorescent probe for fluorescein isothiocyanate (FITC) dextran (Ex=495 nm; Em=525 nm). FITC-Dextran (MW 4000) can be used as a marker to reveal heat shock-induced cell damage and to study the early and late stages of apoptosis. FITC-Dextran (MW 4000) can also be used for cell permeability studies, such as blood-brain barrier permeability and determination of the extent of blood-brain barrier disruption^[1]^[2]^[3].
In Vitro	Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs). Labeling of cells^[1]: For use with apoptotic HeLa cells and human peripheral blood mononuclear cells (PBMC) (viable HeLa and PBMC can not be stained by FITC-Dextran). 1. Incubate cells at 43.5°C for 1 hour and at 37°C for 8 hours to induce apoptosis. 2. Suspend the cells in 100 μL of medium, and mix in Q-prep tubes with 10 μL of propidium iodide (PI), 10 μL of FITC-Dextran (MW 4000) (the final concentration of PI and FITC-Dextran (MW 4000) is 7.5 μM and 1.13 μM, respectively). 3. Incubate cells for 25 min at room temperature in the dark. 4. Take the labeled cells with 3 mL of medium and centrifuge for 10 min at 500 g. 5. Take centrifuged cells with 1 mL of medium and use flow cytometry or fluorescence microscopy analyze (PI: Ex=500 nm, Em=600 nm; FITC-Dextran (MW 4000): Ex=495 nm, Em=525 nm). Paracellular permeability measurement^[4] 1. Add FITC-dextran (0.1 mg/mL) to the basal media in the transwell chamber. 2. Collect media from the transwell insert after 15 min. 3. Measure the fluorescence signal (Ex=485 nm, Em=538 nm). 4. Calculate FITC-dextran concentration based on fluorescence intensity. 5. Calculate permeability. MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. FITC-Dextran (MW 4000) Related Antibodies
In Vivo	Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs). For intestinal barrier function assay^[5] 1. Fast mice for 4 h. 2. Orally gavage mice with FITC-Dextran MW 4000 (0.6 mg/g). 3. Measure fluorescence intensity of plasma in 4 h (excitation nm/emission 520 nm). MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Molecular Weight	4000.00(Average)
CAS No.	60842-46-8
Appearance	Solid
Color	Yellow to orange
Emission (Em)	525
Excitation (Ex)	488
SMILES	[FITC-Dextran (MW 4000)]
Shipping	Room temperature in continental US; may vary elsewhere.
Storage	4°C, protect from light *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Solvent & Solubility	In Vitro: H₂O : 16.67 mg/mL (Need ultrasonic) DMSO : 12.5 mg/mL (ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO) Molarity Calculator Dilution Calculator Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol) Mass = Concentration × Volume × Molecular Weight * Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final) This equation is commonly abbreviated as: C₁V₁ = C₂V₂ Concentration _(start) C1 × Volume _(start) V1 = Concentration _(final) C2 × Volume _(final) V2 In Vivo: Select the appropriate dissolution method based on your experimental animal and administration route. For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents: To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day. The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution. Protocol 1 Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline Solubility: 1.67 mg/mL; Clear solution; Need ultrasonic This protocol yields a clear solution of 1.67 mg/mL. Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (16.7 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL. Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution. Protocol 2 Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline) Solubility: 1.67 mg/mL; Suspended solution; Need ultrasonic This protocol yields a suspended solution of 1.67 mg/mL. Suspended solution can be used for oral and intraperitoneal injection. Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (16.7 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear. Protocol 3 Add each solvent one by one: 10% DMSO 90% Corn Oil Solubility: 1.67 mg/mL; Clear solution; Need ultrasonic This protocol yields a clear solution of 1.67 mg/mL. If the continuous dosing period exceeds half a month, please choose this protocol carefully. Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (16.7 mg/mL) to 900 μL Corn oil, and mix evenly. For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time. The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution. Protocol 1 Add each solvent one by one: PBS Solubility: 100 mg/mL; Clear solution; Need ultrasonic In Vivo Dissolution Calculator Please enter the basic information of animal experiments: Dosage mg/kg Animal weight (per animal) g Dosing volume (per animal) μL Number of animals Recommended: Prepare an additional quantity of animals to account for potential losses during experiments. Calculation results: Working solution concentration: mg/mL This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details. The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE). Tel: 609-228-6898 E-mail: sales@medchemexpress.com Technical Support: tech@medchemexpress.com
Purity & Documentation	Select Batch: Data Sheet (280 KB) SDS (393 KB) COA (253 KB) Handling Instructions (2659 KB)
Dyeing Example	Description: FITC-Dextran MW 4000 can be used to explore the cellular uptake with green fluorescence. Method: For cellular uptake monitoring. 1. Incubate cells in glass dishes (37℃; overnight). 2. Incubate cells with FITC-Dextran MW 4000 (5 μg/mL; 4 h). 3. Wash cells for twice with PBS and finally fix cells with 4% PFA (30 min; dark). 4. Use a confocal laser scanning microscopy (Zeiss 880) for image. Description: FITC-Dextran MW 4000 can be used to detect intestinal barrier function in vivo. Method: For assess intestinal barrier function. 1. Fast mice for 4 hours. 2. Orally gavage mice with FITC-Dextran MW 4000 (0.6 mg/g). 3. Measure fluorescence intensity of plasma in 4 hours. Description: FITC-Dextran MW 4000 can be used for labeling the microvessels in the lesion area of rat brain to measure microvessel density. Method: For vivo labeling. 1. Anesthetize rats first. 2. Inject FITC-dextran FITC-Dextran MW 4000 (50 mg/kg) in tail vein of rats. 3. Remove brain tissues 1 min after injection, use 4% paraformaldehyde (4°C; 24 h) to fix tissues and cut tissues into 150 µm-thick sections. 4. Use a laser scanning confocal microscope (Leica, Solms, Germany) for image.
References	[1]. Moumaris M, et al. Fluorescein isothiocyanate-dextran can track apoptosis and necrosis induced by heat shock of peripheral blood mononuclear cells and HeLa cells[J]. Open Biological Sciences Journal, 2015, 1(1). [2]. Natarajan R, et al. Fluorescein Isothiocyanate (FITC)-Dextran Extravasation as a Measure of Blood-Brain Barrier Permeability. Curr Protoc Neurosci. 2017 Apr 10;79:9.58.1-9.58.15. [Content Brief] [3]. Eriksson I, et al. Analysis of Lysosomal pH by Flow Cytometry Using FITC-Dextran Loaded Cells. Methods Mol Biol. 2017;1594:179-189. [Content Brief] [4]. Okabayashi K, et al. Cdc42 activates paracellular transport in polarised submandibular gland cells. Arch Oral Biol. 2021 Dec;132:105276. [Content Brief] [5]. Yu W, et al. ACE2 contributes to the maintenance of mouse epithelial barrier function. Biochem Biophys Res Commun. 2020 Dec 17;533(4):1276-1282. [Content Brief]