FKBP12 PROTAC dTAG-13 (Synonyms: dTAG-13)

Name: FKBP12 PROTAC dTAG-13
Brand: MedChemExpress
SKU: HY-114421
Rating: 5.0 (2 reviews)

Cat. No.: HY-114421 Purity: 99.75%: FAQs
Data Sheet SDS COA Handling Instructions

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FKBP12 PROTAC dTAG-13 (dTAG-13), a PROTAC-based heterobifunctional degrader, is a selective degrader of FKBP12^F36V with expression of FKBP12F36V in-frame with a protein of interest. FKBP12 PROTAC dTAG-13 effectively engages FKBP12^F36V and CRBN, thereby selectively degrading FKBP12^F36V.

For research use only. We do not sell to patients.

FKBP12 PROTAC dTAG-13 Chemical Structure

CAS No. : 2064175-41-1

Size	Price	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	USD 1016	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 1016	In-stock	Estimated Time of Arrival: December 31
Solid
1 mg	USD 261	In-stock	Estimated Time of Arrival: December 31
5 mg	USD 550	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 880	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 1760	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 2816	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 4505	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

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Customer Review

Based on 2 publication(s) in Google Scholar

2 Publications Citing Use of MCE FKBP12 PROTAC dTAG-13

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Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target^[1]

Cereblon

FKBP12(F36V)

In Vitro

TAG-13 (1-1000 nM; 4 hours; 293FT^WT cells) treatment potently reduces FKBP12^F36V-Nluc levels in 293FT^WT cell, indicating the requirement of CRBN for the observed effects^[1].
Treatment of MV4;11 cells expressing BRD4(short)-FKBP12^F36V with dTAG-13 leads to robust degradation of BRD4. dTAG-13 treatment leads to rapid degradation of BRD4 within one hou. dTAG-13 treatment leads to rapid and potent degradation of the BRD4 fusion chimera in the heterozygous and homozygous knock-in clones, with no effect on endogenous FKBP12^WT^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[1]

Cell Line:	293FT^WT cells
Concentration:	1 nM, 10 nM, 100 nM, 1000 nM
Incubation Time:	4 hours
Result:	Potently reduced FKBP12^F36V-Nluc levels in 293FT^WT cell.

In Vivo

Following bone marrow engraftment of MV4;11 cells expressing luc-FKBP12F36V in mice, the bioluminescent signal after vehicle or dTAG-13 administration is monitored. A significant, rapid, and durable effect on bioluminescent signal is observed four hours after dTAG-13 administration, indicating effective degradation of luc-FKBP12F36V. Twenty-eight hours following the final treatment, the recovery of cellular bioluminescence to levels comparable between vehicle and dTAG-13 treatment groups is observed^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

1049.17

Formula

C₅₇H₆₈N₄O₁₅

CAS No.

2064175-41-1

Appearance

Solid

Color

White to light yellow

SMILES

COC1=CC=C(CC[C@H](C2=C(OCC(NCCCCCCOC3=CC=CC4=C3C(N(C5CCC(NC5=O)=O)C4=O)=O)=O)C=CC=C2)OC([C@@H]6CCCCN6C([C@@H](CC)C7=CC(OC)=C(OC)C(OC)=C7)=O)=O)C=C1OC

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : ≥ 100 mg/mL (95.31 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	0.9531 mL	4.7657 mL	9.5313 mL
5 mM	0.1906 mL	0.9531 mL	1.9063 mL
10 mM	0.0953 mL	0.4766 mL	0.9531 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (2.38 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.75%

Select Batch:

Data Sheet (274 KB) SDS (252 KB)

COA (248 KB) HNMR (248 KB) RP-HPLC (276 KB) LCMS (110 KB)

Handling Instructions (2659 KB)

References

[1]. Nabet B, et al. The dTAG system for immediate and target-specific protein degradation. Nat Chem Biol. 2018 May;14(5):431-441. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	0.9531 mL	4.7657 mL	9.5313 mL	23.8284 mL
	5 mM	0.1906 mL	0.9531 mL	1.9063 mL	4.7657 mL
	10 mM	0.0953 mL	0.4766 mL	0.9531 mL	2.3828 mL
	15 mM	0.0635 mL	0.3177 mL	0.6354 mL	1.5886 mL
	20 mM	0.0477 mL	0.2383 mL	0.4766 mL	1.1914 mL
	25 mM	0.0381 mL	0.1906 mL	0.3813 mL	0.9531 mL
	30 mM	0.0318 mL	0.1589 mL	0.3177 mL	0.7943 mL
	40 mM	0.0238 mL	0.1191 mL	0.2383 mL	0.5957 mL
	50 mM	0.0191 mL	0.0953 mL	0.1906 mL	0.4766 mL
	60 mM	0.0159 mL	0.0794 mL	0.1589 mL	0.3971 mL
	80 mM	0.0119 mL	0.0596 mL	0.1191 mL	0.2979 mL

FKBP12 PROTAC dTAG-13 Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

FKBP12 PROTAC dTAG-132064175-41-1dTAG-13dTAG13dTAG 13PROTACsFKBPFK506-binding proteinBegradationCRBNFKBP12F36Vheterodimerizers rapidBRD4Inhibitorinhibitorinhibit

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FKBP12 PROTAC dTAG-13 (Synonyms: dTAG-13)

Customer Review

FKBP12 PROTAC dTAG-13 Related Antibodies

2 Publications Citing Use of MCE FKBP12 PROTAC dTAG-13

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All PROTACs Isoform Specific Products:

Biological Activity

Purity & Documentation

References

Customer Review

FKBP12 PROTAC dTAG-13 Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

FKBP12 PROTAC dTAG-13 Related Classifications

Keywords:

Your Recently Viewed Products:

Customer Review

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SAFETY DATA SHEET (SDS)

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