2. Stem Cell/Wnt
  3. Wnt
  4. Fz7-21 TFA

Fz7-21 TFA  (Synonyms: Ac-LPSDDLEFWCHVMY-NH2 TFA)

Cat. No.: HY-P1454A Purity: 99.35%
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Fz7-21 (Ac-LPSDDLEFWCHVMY-NH2) TFA is a potent peptide antagonist of FZD7 receptors , selectively binds to FZD7 CRD subclass and alters the conformation of the CRD and the architecture of its lipid-binding groove. The EC50 values are 58 and 34 nM for human and mouse FZD7 CRD, respectively. Fz7-21 TFA impairs the function of FZD7 in Wnt-β-catenin signalling and stem cell function in intestinal organoids.

For research use only. We do not sell to patients.

Custom Peptide Synthesis

Fz7-21 TFA Chemical Structure

Fz7-21 TFA Chemical Structure

Size Price Stock Quantity
1 mg USD 85 In-stock
5 mg USD 220 In-stock
10 mg USD 350 In-stock
25 mg USD 595 In-stock
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Customer Review

Based on 7 publication(s) in Google Scholar

Other Forms of Fz7-21 TFA:

Fz7-21 TFA Related Antibodies

Top Publications Citing Use of Products

    Fz7-21 TFA purchased from MedChemExpress. Usage Cited in: Cell Prolif. 2023 Mar 27;e13460.  [Abstract]

    Fz7-21 (200 nM; 14 days) inhibits the release of Ca2+ induced by WNT16b in hLESCs.

    Fz7-21 TFA purchased from MedChemExpress. Usage Cited in: Cell Prolif. 2023 Mar 27;e13460.  [Abstract]

    Fz7-21 (200 nM; 14 days) suppresses hLESC proliferation induced by WNT16b in hLESCs.

    Fz7-21 TFA purchased from MedChemExpress. Usage Cited in: Cell Prolif. 2023 Mar 27;e13460.  [Abstract]

    Fz7-21 (200 nM; 14 days) decreases the expression of Calcineurin A and NFATC2 in hLESCs.

    Fz7-21 TFA purchased from MedChemExpress. Usage Cited in: Cell Prolif. 2023 Mar 27;e13460.  [Abstract]

    Fz7-21 (200 nM; 14 days) downregulates the expression of the stemness biomarker ΔNp63α in hLESCs.

    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Fz7-21 (Ac-LPSDDLEFWCHVMY-NH2) TFA is a potent peptide antagonist of FZD7 receptors , selectively binds to FZD7 CRD subclass and alters the conformation of the CRD and the architecture of its lipid-binding groove. The EC50 values are 58 and 34 nM for human and mouse FZD7 CRD, respectively. Fz7-21 TFA impairs the function of FZD7 in Wnt-β-catenin signalling and stem cell function in intestinal organoids[1][2].

    IC50 & Target

    EC50: 58 nM (human FZD7 CRD), 34 nM (mouse FZD7 CRD)[1]
    In Vitro

    Fz7-21 (Ac-LPSDDLEFWCHVMY-NH2) TFA (0-100 μM; 6 h; HEK293-TB cells) impairs Wnt signaling with IC50 value of 100 nM[1].
    Fz7-21 (Ac-LPSDDLEFWCHVMY-NH2) TFA (1 µM) blocks WNT3A-mediated stabilization of β-catenin in mouse L cells with IC50 value of 50 nM[1].
    Fz7-21 (Ac-LPSDDLEFWCHVMY-NH2) TFA (200 µM; 48 h; LGR5–GFP+ stem cells) disrupts LGR5+ stem cell function[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Fz7-21 TFA Related Antibodies
    Molecular Weight

    1910.07

    Formula

    C85H115N18F3O25S2
    Appearance

    Solid

    Color

    White to off-white

    Sequence Shortening

    Ac-LPSDDLEFWCHVMY-NH2
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    Sealed storage, away from moisture

    Powder -80°C 2 years
    -20°C 1 year

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

    Solvent & Solubility
    In Vitro: 

    DMSO : 33.33 mg/mL (17.45 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    H2O : < 0.1 mg/mL (insoluble)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 0.5235 mL 2.6177 mL 5.2354 mL
    5 mM 0.1047 mL 0.5235 mL 1.0471 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
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    Concentration
    ×
    Volume
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    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

    =
    Concentration (final)

    C2

    ×
    Volume (final)

    V2

    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: 2.5 mg/mL (1.31 mM); Suspended solution; Need ultrasonic

      This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% Corn Oil

      Solubility: ≥ 2.5 mg/mL (1.31 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    Purity & Documentation
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 0.5235 mL 2.6177 mL 5.2354 mL 13.0885 mL
    5 mM 0.1047 mL 0.5235 mL 1.0471 mL 2.6177 mL
    10 mM 0.0524 mL 0.2618 mL 0.5235 mL 1.3089 mL
    15 mM 0.0349 mL 0.1745 mL 0.3490 mL 0.8726 mL
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    Fz7-21 TFA Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Fz7-21Ac-LPSDDLEFWCHVMY-NH2WntFZD7conformationlipid-binding grooveFZD7 CRDWnt–β-cateninintestinal organoidsInhibitorinhibitorinhibit

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