DFFB - DNA fragmentation factor subunit beta Gene

Homo sapiens

Also known as CAD; CPAN; DFF2; DFF40; DFF-40

Gene ID: 1677 | Gene type: protein coding

About DFFB

Cytogenetic location: 1p36.32 Genomic coordinates (GRCh38): 1:3,857,476-3,885,429 (from NCBI)

This gene has 11 transcripts (splice variants) and 205 orthologues. Ubiquitous expression in duodenum (RPKM 1.4), spleen (RPKM 1.3) and 25 other tissues.

Summary

Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for Caspase-3 and triggers DNA fragmentation during Apoptosis. DFF becomes activated when DFFA is cleaved by Caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during Apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene but the biological validity of some of these variants has not been determined. [provided by RefSeq, Sep 2013]

DFFB Products(4)

mRNA	Protein	Name
NM_001282669.2	NP_001269598.1	DNA fragmentation factor subunit beta isoform 1
NM_001320132.2	NP_001307061.1	DNA fragmentation factor subunit beta isoform 4
NM_001320136.2	NP_001307065.1	DNA fragmentation factor subunit beta isoform 5
NM_004402.4	NP_004393.1	DNA fragmentation factor subunit beta isoform 2

Gene Ontology

Molecular Function
Biological Process
Cellular Component

Molecular Function GO Annotation	Evidence	Reference	Source
enables DNA nuclease activity	IMP IMP: Inferred from mutant phenotype	19944011	GOA
enables disordered domain specific binding	IPI IPI: Inferred from physical interaction	11371636	GOA
enables enzyme binding	IPI IPI: Inferred from physical interaction	10959840	GOA
enables identical protein binding	IPI IPI: Inferred from physical interaction	11371636	GOA
enables protein binding	IPI IPI: Inferred from physical interaction	11371636	GOA
enables protein domain specific binding	IPI IPI: Inferred from physical interaction	19944011	GOA

Biological Process GO Annotation	Evidence	Reference	Source
involved in DNA catabolic process	IMP IMP: Inferred from mutant phenotype	19944011	GOA
acts upstream of or within apoptotic DNA fragmentation	IDA IDA: Inferred from direct assay	15572351	GOA
involved in apoptotic DNA fragmentation	IDA IDA: Inferred from direct assay	22253444	GOA
involved in apoptotic chromosome condensation	IDA IDA: Inferred from direct assay	10959840	GOA
involved in negative regulation of apoptotic DNA fragmentation	IDA IDA: Inferred from direct assay	11371636	GOA

Cellular Component GO Annotation	Evidence	Reference	Source
located in chromatin	IDA IDA: Inferred from direct assay	19882353	GOA
located in cytosol	IDA IDA: Inferred from direct assay	9108473	GOA
located in nucleus	IDA IDA: Inferred from direct assay	10959840	GOA
part of protein-containing complex	IDA IDA: Inferred from direct assay	11371636	GOA

EXP: Inferred from Experiment IDA: Inferred from direct assay IPI: Inferred from physical interaction IMP: Inferred from mutant phenotype IGI: Inferred from genetic interaction IEP: Inferred from expression pattern

DFFB Protein Structure

CIDE-N

DFF40

0
100
200
300
338 a.a.

Protein Preferred Names

Protein Names

DNA fragmentation factor subunit beta

DNA fragmentation factor 40 kDa subunit

DFFB Protein-protein interaction Information

Type Cross Intra	Protein Name	Protein ID	Interactor	Interactor Species	Interactor ID	Detection Method	Reference
Intra	DFFB	O76075	DFFA	Homo sapiens	O00273	Anti Tag CoIP	33961781
Intra	DFFB	O76075	DFFA	Homo sapiens	O00273	Y2H Prey Pooling	32296183
Intra	DFFB	O76075	DFFA	Homo sapiens	O00273	Y2H Array	32296183
Intra	DFFB	O76075	DFFA	Homo sapiens	O00273	CoIP	9108473

Cross: Cross-species interaction Intra: Intraspecies interaction

Related Diseases

Diseases

Alias

Huntington Disease

Huntington'S Disease	Huntington Chorea
Huntington'S Chorea	HD
Huntington Chronic Progressive Hereditary Chorea	Juvenile Huntington Disease
Chronic Progressive Chorea	Chronic Progressive Hereditary Chorea
Hc - [Huntington Chorea]	Hereditary Chorea
Progressive Hereditary Chorea

Diseases	Alias
Waldenstroem'S Macroglobulinemia	Waldenstroem'S Macroglobulinemia Waldenstroem'S Macroglobulinemia Macroglobulinemia Of Waldenstrom Lymphoplasmacytic Lymphoma With Igm Gammopathy Lymphoplasmacytic Lymphoma Waldenstroem'S Macroglobulinemia Waldenstroem'S Macroglobulinemia Macroglobulinemia Of Waldenstrom Lymphoplasmacytic Lymphoma With Igm Gammopathy Lymphoplasmacytic Lymphoma

Related Agents

Pre-clinical Phase

Bioactive Molecules (1)

Cat. No.	Product Name	Effect	Purity	Rare Diseases
HY-RS03709	DFFB Human Pre-designed siRNA Set A	Pre-designed Sets	/	Unkown

Orthologs Information

Species	Symbol	Source	ID
Mus musculus	DFFB	MGD	MGI:1196287
Bos taurus	DFFB	VGNC	VGNC:28018
Rattus norvegicus	DFFB	RGD	RGD:620335
Macaca mulatta	DFFB	VGNC	VGNC:99891
Felis catus	DFFB	VGNC	VGNC:61454
Canis familiaris	DFFB	VGNC	VGNC:39910

Name
Email *

	Sorry, but the email address you supplied was invalid.