2. Peptides
  3. Peptide and Derivatives
  4. Inhibitors and Substrates
  5. Enzyme Substrates
  6. H-Ala-Pro-AFC

H-Ala-Pro-AFC is a biological active peptide. (This is a fluorescent peptide, Abs/Em=380/500nm. It is a substrate for dipeptidyl peptidase IV (DPP IV) and Xaa-Pro dipeptidase.)

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H-Ala-Pro-AFC Chemical Structure

H-Ala-Pro-AFC Chemical Structure

CAS No. : 125791-92-6

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5 mg USD 285 In-stock
10 mg USD 455 In-stock
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Based on 1 publication(s) in Google Scholar

H-Ala-Pro-AFC Related Antibodies

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Description

H-Ala-Pro-AFC is a biological active peptide. (This is a fluorescent peptide, Abs/Em=380/500nm. It is a substrate for dipeptidyl peptidase IV (DPP IV) and Xaa-Pro dipeptidase.)

Molecular Weight

397.35

Formula

C18H18F3N3O4
CAS No.
Appearance

Solid

Color

Off-white to light yellow

Sequence

Ala-Pro-{AFC}
Sequence Shortening

AP-{AFC}
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Sealed storage, away from moisture and light

Powder -80°C 2 years
-20°C 1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility
In Vitro: 

DMSO : ≥ 100 mg/mL (251.67 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.5167 mL 12.5834 mL 25.1667 mL
5 mM 0.5033 mL 2.5167 mL 5.0333 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
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Volume
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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

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g

Dosing volume
(per animal)

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Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.5167 mL 12.5834 mL 25.1667 mL 62.9168 mL
5 mM 0.5033 mL 2.5167 mL 5.0333 mL 12.5834 mL
10 mM 0.2517 mL 1.2583 mL 2.5167 mL 6.2917 mL
15 mM 0.1678 mL 0.8389 mL 1.6778 mL 4.1945 mL
20 mM 0.1258 mL 0.6292 mL 1.2583 mL 3.1458 mL
25 mM 0.1007 mL 0.5033 mL 1.0067 mL 2.5167 mL
30 mM 0.0839 mL 0.4194 mL 0.8389 mL 2.0972 mL
40 mM 0.0629 mL 0.3146 mL 0.6292 mL 1.5729 mL
50 mM 0.0503 mL 0.2517 mL 0.5033 mL 1.2583 mL
60 mM 0.0419 mL 0.2097 mL 0.4194 mL 1.0486 mL
80 mM 0.0315 mL 0.1573 mL 0.3146 mL 0.7865 mL
100 mM 0.0252 mL 0.1258 mL 0.2517 mL 0.6292 mL
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H-Ala-Pro-AFC Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

H-Ala-Pro-AFC125791-92-6OthersInhibitorinhibitorinhibit

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