2. Immunology/Inflammation NF-κB Metabolic Enzyme/Protease
  3. Reactive Oxygen Species
  4. HKSOX-1r (5/6-mixture)

HKSOX-1r (5/6-mixture) is a fluorescent probe which is used for imaging and detection of endogenous superoxide in live cells and in vivo. HKSOX-1r (5/6-mixture) exhibits excellent selectivity and sensitivity towards superoxide anion radical.

For research use only. We do not sell to patients.

HKSOX-1r (5/6-mixture) Chemical Structure

HKSOX-1r (5/6-mixture) Chemical Structure

Size Price Stock Quantity
1 mg USD 375 In-stock
5 mg USD 990 In-stock
10 mg USD 1628 In-stock
25 mg USD 3256 In-stock
50 mg USD 4884 In-stock
100 mg USD 7326 In-stock
200 mg   Get quote  
500 mg   Get quote  

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 1 publication(s) in Google Scholar

HKSOX-1r (5/6-mixture) Related Antibodies

Top Publications Citing Use of Products

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

HKSOX-1r (5/6-mixture) is a fluorescent probe which is used for imaging and detection of endogenous superoxide in live cells and in vivo. HKSOX-1r (5/6-mixture) exhibits excellent selectivity and sensitivity towards superoxide anion radical[1].

In Vitro

HKSOX-1r 5/6-mixture (2 μM; 30 min) detects mitochondrial respiratory inhibitor-induced O2•− formation in a highly sensitive and rapid manner in HCT116, BV-2 and RAW264.7 cells[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

HKSOX-1r (5/6-mixture) Related Antibodies
In Vivo

HKSOX-1r 5/6-mixture (10 μM; 20 min) detected obvious fluorescence distribution in zebrafish embryos challenged with PMA or Antimycin A[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Molecular Weight

855.54

Formula

C29H15F10NO14S2
Appearance

Solid

Emission (Em)

534
Excitation (Ex)

509
SMILES

O=C(C1=CC2=C(C3(C4=C(OC5=C3C=C(F)C(OS(=O)(C(F)(F)F)=O)=C5F)C(F)=C(OS(=O)(C(F)(F)F)=O)C(F)=C4)OC2=O)C=C1)N(CC(OC)=O)CC(OC)=O.O=C(C6=CC(C7(C8=C(OC9=C7C=C(F)C(OS(=O)(C(F)(F)F)=O)=C9F)C(F)=C(OS(=O)(C(F)(F)F)=O)C(F)=C8)O%10)=C(C=C6)C%10=O)N(CC(OC)=O)CC(OC)=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 200 mg/mL (233.77 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.1689 mL 5.8443 mL 11.6885 mL
5 mM 0.2338 mL 1.1689 mL 2.3377 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    90% Corn Oil

    Solubility: ≥ 5 mg/mL (5.84 mM); Clear solution

    This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 98.68%

COA (255 KB) HNMR (428 KB) LCMS (101 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.1689 mL 5.8443 mL 11.6885 mL 29.2213 mL
5 mM 0.2338 mL 1.1689 mL 2.3377 mL 5.8443 mL
10 mM 0.1169 mL 0.5844 mL 1.1689 mL 2.9221 mL
15 mM 0.0779 mL 0.3896 mL 0.7792 mL 1.9481 mL
20 mM 0.0584 mL 0.2922 mL 0.5844 mL 1.4611 mL
25 mM 0.0468 mL 0.2338 mL 0.4675 mL 1.1689 mL
30 mM 0.0390 mL 0.1948 mL 0.3896 mL 0.9740 mL
40 mM 0.0292 mL 0.1461 mL 0.2922 mL 0.7305 mL
50 mM 0.0234 mL 0.1169 mL 0.2338 mL 0.5844 mL
60 mM 0.0195 mL 0.0974 mL 0.1948 mL 0.4870 mL
80 mM 0.0146 mL 0.0731 mL 0.1461 mL 0.3653 mL
100 mM 0.0117 mL 0.0584 mL 0.1169 mL 0.2922 mL
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HKSOX-1r (5/6-mixture) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

HKSOX-1r (5/6-mixture)Reactive Oxygen SpeciesfluorescentprobesuperoxideanionradicalInhibitorinhibitorinhibit

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