HSP70-IN-3

Cat. No.: HY-143400: FAQs
Data Sheet Handling Instructions

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HSP70-IN-3 is a potent HSP70 inhibitor (IC₅₀s of 1.1 and 1.9 μM in ASZ001 and C3H10T1/2, respectively). HSP70-IN-3 has anti-Hh (Hedgehog signaling) activity and anti-proliferative activity and reduces expression of the oncogenic transcription factor GLI1.

For research use only. We do not sell to patients.

HSP70-IN-3 Chemical Structure

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Biological Activity
Purity & Documentation
References
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Description

IC₅₀ & Target

IC₅₀: 1.1 μM (HSP70) in ASZ001, 1.9 μM (HSP70) in C3H10T1/2^[1]

In Vitro

HSP70-IN-3 (compound 8) (5 μM; 72 hours) has anti-Hh (Hedgehog signaling) activity and anti-proliferative activity in ASZ001 cells^[1].
HSP70-IN-3 can almost completely abolish the expression of Gli1 mRNA in ASZ001 cells after 20-hour incubation^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Proliferation Assay

Cell Line:	ASZ001 cells^[1]
Concentration:	5 μM
Incubation Time:	72 hours
Result:	Showed anti-Hh(Hedgehog signaling) activity and anti-proliferative activity.

Cell Proliferation Assay

Cell Line:	ASZ001 cells^[1]
Concentration:	10 μM
Incubation Time:	48 hours
Result:	After a 20-h incubation with HSP70-IN-3, expression of Gli1 mRNA was almost completely abolished.

Molecular Weight

883.23

Formula

C₄₈H₇₈N₆O₇S

SMILES

[H][C@@]12CS[C@@H]([C@@]1(NC(N2)=O)[H])CCCCC(NCCOCCOCCOCCN3C=C(N=N3)CO[C@@H]([C@H]([C@H]4CC[C@]5(/C(CCC[C@]45C)=C/C=C6C[C@H](CCC/6=C)O)[H])C)CCC(C)C)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation

Handling Instructions (2659 KB)

References

[1]. Wen J, et al. Affinity-based protein profiling identifies vitamin D3 as a heat shock protein 70 antagonist that regulates hedgehog transduction in murine basal cell carcinoma. Eur J Med Chem. 2022;228:114005. [Content Brief]

HSP70-IN-3 Related Classifications

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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The dilution calculator equation

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

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	×		=		×
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Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

HSP70-IN-3HSPHedgehogHeat shock proteinsVitamin D3Affinity-based proteinHeat shock protein 70Hedgehog pathwayBasal cell carcinomaInhibitorinhibitorinhibit

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