Hygrolidin

Cat. No.: HY-133537: FAQs
Data Sheet Handling Instructions

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Hygrolidin is a 16-membered macrolide antibiotic produced by Streptomyces hygroscopicus D-1166. Hygrolidin has anti-fungus activity against Valsa ceratosperma. Hygrolidin induces p21 expression and abrogates cell cycle progression at G1 and S phases. Hygrolidin has antitumor activity.

For research use only. We do not sell to patients.

Hygrolidin Chemical Structure

CAS No. : 83329-73-1

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5 mg	Get quote	Estimated Time of Arrival: December 31
10 mg	Get quote	Estimated Time of Arrival: December 31
25 mg	Get quote	Estimated Time of Arrival: December 31

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Biological Activity
Purity & Documentation
References
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Description

IC₅₀ & Target

Macrolide

In Vitro

Hygrolidin (0.1, 1 μg/ml; for 24 h) increases both G1 and S phase populations and decreases M phase population^[1].
Hygrolidin (0.1, 1, 10 μg/ml; for 24 h) decreases the amounts of cdk4, cyclin D, cyclin B and increases the amounts of cyclin E and p21. Hygrolidin-induced p21 preferentially associates with cyclin A-cdk2 complex and inhibits it^[1].
Hygrolidin (0.1, 1 μg/ml; for 24 h) selectively induces p21 in DLD-1 cells at mRNA level, but not in WI-38 fibroblasts^[1].
Hygrolidin, for 3 days, inhibits growth of various cell lines including DLD-1 colon cancer, LNCaP prostate cancer, K562 leukemia cells, LNCaP prostate cancer, EL-4 lymphoma (IC₅₀s = 1.0-33 ng/ml, respectively)^[1].
Hygrolidin shows growth inhibition on Trypanosoma cruzi (IC₅₀=1.1 nM), Trypanosoma brucei brucei (IC₅₀=77 nM), and Leishmania donovani (IC₅₀=72.5 nM)^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Cycle Analysis^[1]

Cell Line:	DLD-1 cells
Concentration:	0.1, 1 μg/ml
Incubation Time:	For 24 hours
Result:	Increased both G1 and S phase populations and decreased M phase population.

Western Blot Analysis^[1]

Cell Line:	DLD-1 cells
Concentration:	0.1, 1, 10 μg/ml
Incubation Time:	For 24 hours
Result:	Decreased the amounts of cdk4, cyclin D, cyclin B and increased the amounts of cyclin E and p21.

RT-PCR^[1]

Cell Line:	DLD-1 or WI-38 cells
Concentration:	0.1, 1 μg/ml
Incubation Time:	For 24 hours
Result:	Selectively induced p21 in DLD-1 cells at mRNA level, but not in WI-38 fibroblasts.

Molecular Weight

690.86

Formula

C₃₈H₅₈O₁₁

CAS No.

83329-73-1

SMILES

O=C(O[C@@H]1C[C@@]([C@@H](C)[C@H](O)[C@@H]([C@H]([C@@H](OC)/C=C/C=C(C)/C[C@H](C)[C@H](O)[C@H](C)/C=C(C)/C=C2\C)OC2=O)C)(O)O[C@H](CC)[C@@H]1C)/C=C/C(O)=O

Structure Classification

Initial Source

Microorganisms

Streptomyces hygroscopicus

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation

Handling Instructions (2659 KB)

References

[1]. Manabu Kawada, et al. Hygrolidin induces p21 expression and abrogates cell cycle progression at G1 and S phases. Biochem Biophys Res Commun. 2002 Oct 18;298(1):178-83. [Content Brief]

[2]. H Seto, et al. The isolation and structures of hygrolidin amide and defumarylhygrolidin. J Antibiot (Tokyo). 1984 May;37(5):610-3. [Content Brief]

[3]. F Annang, et al. High-throughput screening platform for natural product-based drug discovery against 3 neglected tropical diseases: human African trypanosomiasis, leishmaniasis, and Chagas disease. J Biomol Screen. 2015 Jan;20(1):82-91. [Content Brief]

Hygrolidin Related Classifications

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Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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