icFSP1

Cat. No.: HY-157068 Purity: 99.72%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

icFSP1 is a potent ferroptosis suppressor protein-1 (FSP1) inhibitor. icFSP1 does not competitively inhibit FSP1 enzyme activity, but instead triggers subcellular relocalization of FSP1 from the membrane and FSP1 condensation before ferroptosis induction, in synergism with GPX4 inhibition.

For research use only. We do not sell to patients.

icFSP1 Chemical Structure

CAS No. : 1115910-36-5

Size	Price	Stock	Quantity
1 mg	USD 350	In-stock	Estimated Time of Arrival: December 31
5 mg	USD 800	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 1120	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 1980	In-stock	Estimated Time of Arrival: December 31
100 mg		Get quote
200 mg		Get quote

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Purity & Documentation
References
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Description

Molecular Weight

459.49

Formula

C₂₆H₂₅N₃O₅

CAS No.

1115910-36-5

Appearance

Solid

Color

White to yellow

SMILES

O=C1C2=C(N=C(C)N1C3=CC=C(NC(CC4=CC(OC)=C(C(OC)=C4)OC)=O)C=C3)C=CC=C2

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (217.63 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.1763 mL	10.8816 mL	21.7633 mL
5 mM	0.4353 mL	2.1763 mL	4.3527 mL
10 mM	0.2176 mL	1.0882 mL	2.1763 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: 2.5 mg/mL (5.44 mM); Clear solution; Need ultrasonic

This protocol yields a clear solution of 2.5 mg/mL.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: 2.5 mg/mL (5.44 mM); Clear solution; Need ultrasonic

This protocol yields a clear solution of 2.5 mg/mL. If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.72%

Select Batch:

Data Sheet (270 KB) SDS (252 KB)

COA (247 KB) HNMR (145 KB) RP-HPLC (233 KB) LCMS (104 KB)

Handling Instructions (2659 KB)

References

[1]. Toshitaka Nakamura, et al. Phase separation of FSP1 promotes ferroptosis. Nature. 2023 Jul;619(7969):371-377. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.1763 mL	10.8816 mL	21.7633 mL	54.4081 mL
	5 mM	0.4353 mL	2.1763 mL	4.3527 mL	10.8816 mL
	10 mM	0.2176 mL	1.0882 mL	2.1763 mL	5.4408 mL
	15 mM	0.1451 mL	0.7254 mL	1.4509 mL	3.6272 mL
	20 mM	0.1088 mL	0.5441 mL	1.0882 mL	2.7204 mL
	25 mM	0.0871 mL	0.4353 mL	0.8705 mL	2.1763 mL
	30 mM	0.0725 mL	0.3627 mL	0.7254 mL	1.8136 mL
	40 mM	0.0544 mL	0.2720 mL	0.5441 mL	1.3602 mL
	50 mM	0.0435 mL	0.2176 mL	0.4353 mL	1.0882 mL
	60 mM	0.0363 mL	0.1814 mL	0.3627 mL	0.9068 mL
	80 mM	0.0272 mL	0.1360 mL	0.2720 mL	0.6801 mL
	100 mM	0.0218 mL	0.1088 mL	0.2176 mL	0.5441 mL

icFSP1 Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

icFSP11115910-36-5icFSP 1icFSP-1Ferroptosisferroptosis suppressor protein-1 (FSP1)GPX4Inhibitorinhibitorinhibit

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