1. MCE Kits
  2. Cell Isolation
  3. Mouse CD3+ T Cells Negative Selection Kit

Mouse CD3+ T Cells Negative Selection Kit 

Cat. No.: HY-K0306
Manual SDS

MCE Mouse CD3+ T Cells Negative Selection Kit is designed for the isolation of CD3+ T cells from single cell suspensions of mouse spleen cells and lymph nodes.

Mouse CD3<sup>+</sup> T Cells Negative Selection Kit
Size Price Stock Quantity
1 mL USD 580 In-stock
2 mL USD 965 In-stock

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  • Description

  • Storage

  • Components

  • Documentation

Description
& Advantages

MCE Mouse CD3+ T Cells Negative Selection Kit is designed for the isolation of CD3+ T cells from single cell suspensions of mouse spleen cells and lymph nodes. Its principle hinges on utilizing a biotin-labeled monoclonal antibody to mark non-target cells (non-CD3+ T-cells), which are subsequently removed using streptavidin-labeled magnetic beads. This process effectively facilitates the sorting of human CD3+ T cells.

The kit is user-friendly and portable, with components that are non-toxic to cells. It enables the isolation of high-purity cells, and the resulting cells can be utilized in a variety of downstream applications. These include flow cytometry, cell culture, Western blot analysis, immunoprecipitation (IP), reporter gene detection, and DNA/RNA extraction.

Features of MCE Mouse CD3+ T Cells Negative Selection Kit

•   Efficiency: Enables sorting of target cells in as little as 15 minutes;

•   Separation Columns-free: Eliminates the need for separation columns by using a magnetic separator for swift cell separation;

•   High Purity: Can yield sorted cells with a purity exceeding 95%;

•   High Activity: The sorted cells are free of antibody and magnetic bead labeling, and retain high activity and functionality for downstream experiments.

Storage

4°C,2 years

Do not freeze the magnetic beads

Components
Components HY-K0306-1 mL (For 5 × 108 cells) HY-K0306-2 mL (For 109 cells)
Biotin-Antibody Mix 100 μL 200 μL
Streptavidin Magnetic Beads 1 mL 1 mL × 2
Documentation
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Product Name:
Mouse CD3+ T Cells Negative Selection Kit
Cat. No.:
HY-K0306
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