2. MCE Kits
  3. Protein Biology
  4. Protein Electrophoresis & WB
  5. Electrophoresis Buffer
  6. Tris-HEPES-SDS Powder (500 mL of 1×)

Tris-HEPES-SDS Powder (500 mL of 1×) 

Cat. No.: HY-K1034
Manual SDS

MCE Tris-HEPES-SDS Powder (500 mL of 1×) consists of Tris, HEPES, EDTA and SDS, and is widely used in protein electrophoresis.

Tris-HEPES-SDS Powder (500 mL of 1×)
Size Price Stock Quantity
Free Sample (2 pouches)   Apply Now  
20 pouches USD 98 In-stock
100 pouches USD 290 In-stock
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  • Description

  • Storage

  • Protocol

  • Components

  • Documentation

Description
& Advantages

Tris-HEPES-SDS (HEPES) is widely used in protein electrophoresis, as well as in biochemical diagnostic kits, DNA/RNA extraction kits, PCR diagnostic kits, etc. HEPES is non-toxic to cells.

MCE Tris-HEPES-SDS Powder (500 mL of 1×) consists of Tris, HEPES, EDTA and SDS. Each pouch can be diluted to 500 mL 1× HEPES buffer, in which the concentration of Tris is 50 mM, the concentration of HEPES is 50 mM, the concentration of EDTA is 2 mM and the concentration of SDS is 0.1%. This product provides a convenient way to make HEPES buffer and eliminates the need to weigh and mix individual components.

Storage

Store at room temperature for 3 years.

Protocol

1.   Add 1 pouch of Tris-HEPES-SDS Powder into the cleaned beaker, dissolve with 300 mL distilled water under a magnetic stirrer.

2.   Add distilled water to the solution in step 1 until the total volume is 500 mL. The final solution is 1× HEPES buffer.

Note: The pH of the 1× HEPES buffer is 7.7 ± 0.2 @ 25°C.

Components
Components HY-K1034-20 pouches HY-K1034-100 pouches
Tris-HEPES-SDS Powder (500 mL of 1×) 20 pouches 5 × 20 pouches
Documentation
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Product Name:
Tris-HEPES-SDS Powder (500 mL of 1×)
Cat. No.:
HY-K1034
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