2. GPCR/G Protein Neuronal Signaling
  3. mAChR
  4. Ipratropium bromide hydrate

Ipratropium bromide hydrate  (Synonyms: Sch 1000 bromide hydrate)

Cat. No.: HY-B1332 Purity: 99.82%
COA Handling Instructions

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Ipratropium bromide (Sch 1000) hydrate is a muscarinic receptor antagonist, with IC50s of 2.9 nM, 2 nM, and 1.7 nM for M1, M2, and M3 receptors, respectively. Ipratropium bromide hydrate relaxes smooth muscle, can be used in the research for COPD (chronic obstructive pulmonary disease) and asthma.

For research use only. We do not sell to patients.

Ipratropium bromide hydrate Chemical Structure

Ipratropium bromide hydrate Chemical Structure

CAS No. : 66985-17-9

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500 mg USD 120 In-stock
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Based on 1 publication(s) in Google Scholar

Other Forms of Ipratropium bromide hydrate:

Ipratropium bromide hydrate Related Antibodies

Top Publications Citing Use of Products

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mAChR1 mAChR2 mAChR3 mAChR4 mAChR5

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Ipratropium bromide (Sch 1000) hydrate is a muscarinic receptor antagonist, with IC50s of 2.9 nM, 2 nM, and 1.7 nM for M1, M2, and M3 receptors, respectively. Ipratropium bromide hydrate relaxes smooth muscle, can be used in the research for COPD (chronic obstructive pulmonary disease) and asthma[1][2][3][4][5].

IC50 & Target

mAChR1

2.9 nM (IC50)

mAChR2

2 nM (IC50)

mAChR3

1.7 nM (IC50)

In Vitro

Ipratropium bromide hydrate (1 nM, 10 nM, 100 nM; 15 min) exerts its toxic effects via disruption of mitochondrial membrane potential[1].
Ipratropium bromide hydrate (1 nM-1 μM; 4 h) increases infarct size in isolated perfused heart ischaemia/reperfusion experiments with a dose-responsive manner (EC50=22.7 nM)[1].
Ipratropium bromide hydrate (0.001 nM-0.1 mM; 2 h) inhibits adult rat cardiac myocyte growth after 4 h hypoxia treatment[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Ipratropium bromide hydrate Related Antibodies

Cell Viability Assay[1]

Cell Line: Adult Rat Cardiac Myocyte
Concentration: 0.001 nM-0.1 mM
Incubation Time: 2 h in dark; prior to 4 h hypoxia
Result: Resulted cell viability in a dose-dependent manner, with the inhibition rate of 52.7% at 0.1 mM dose.
In Vivo

Ipratropium bromide hydrate (1.0 μg/kg; i.v.; single dose) enhances vagal nerve stimulation induing bronchoconstriction[2].
Ipratropium bromide hydrate (0.04 mg/20 mL and 0.20 mg/20 mL; inhalation for 30 min, rate=30 mL/30 min) can protect the lungs against the cadmium-induced acute neutrophilic inflammation by reducing the parenchyma inflammatory infiltration of neutrophils[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Guinea-pigs of the Dunkin Hartley strain[2]
Dosage: 0.1-1 μg/kg
Administration: Intravenous injection; single dose
Result: Resulted little blocking effect on post-junctional muscarinic receptors at 0.3 μg/kg, and inhibited ACh-induced bronchoconstriction at 0.5 μg/kg.
Animal Model: Male Sprague-Dawley rats (300-350 g)[4]
Dosage: 0.04 mg/20 mL and 0.20 mg/20 mL
Administration: Inhalation; atomization rate of 30 mL/30 min; 30 min
Result: Had no significant effects on any parameters recorded in healthy rats but exerted a protective effect against the inflammatory reaction elicited by cadmium.
Clinical Trial
Molecular Weight

430.38

Formula

C20H32BrNO4
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

C[N+]1([C@H]2CC[C@H]1CC(OC(C(CO)C3=CC=CC=C3)=O)C2)C(C)C.O.[Br-]
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

H2O : 50 mg/mL (116.18 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.3235 mL 11.6176 mL 23.2353 mL
5 mM 0.4647 mL 2.3235 mL 4.6471 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  PBS

    Solubility: 50 mg/mL (116.18 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation

Purity: 99.82%

COA (257 KB) HNMR (260 KB) LCMS (88 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O 1 mM 2.3235 mL 11.6176 mL 23.2353 mL 58.0882 mL
5 mM 0.4647 mL 2.3235 mL 4.6471 mL 11.6176 mL
10 mM 0.2324 mL 1.1618 mL 2.3235 mL 5.8088 mL
15 mM 0.1549 mL 0.7745 mL 1.5490 mL 3.8725 mL
20 mM 0.1162 mL 0.5809 mL 1.1618 mL 2.9044 mL
25 mM 0.0929 mL 0.4647 mL 0.9294 mL 2.3235 mL
30 mM 0.0775 mL 0.3873 mL 0.7745 mL 1.9363 mL
40 mM 0.0581 mL 0.2904 mL 0.5809 mL 1.4522 mL
50 mM 0.0465 mL 0.2324 mL 0.4647 mL 1.1618 mL
60 mM 0.0387 mL 0.1936 mL 0.3873 mL 0.9681 mL
80 mM 0.0290 mL 0.1452 mL 0.2904 mL 0.7261 mL
100 mM 0.0232 mL 0.1162 mL 0.2324 mL 0.5809 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Ipratropium bromide hydrate Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Ipratropium bromide66985-17-9Sch 1000 bromidemAChRMuscarinic acetylcholine receptorchronic obstructive pulmonary diseaseasthmaischaemia/reperfusionI/RInhibitorinhibitorinhibit

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