Ischemin sodium

Cat. No.: HY-110315 Purity: 99.32%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Ischemin sodium is a CBP bromodomain inhibitor that inhibits p53 interaction with CBP and transcriptional activity in cells. Ischemin sodium salt inhibits p53-induced p21 activation with an IC₅₀ value of 5 µM. Ischemin sodium salt also prevents apoptosis in ischemic cardiomyocytes. Ischemin sodium salt can be used in the study of cardiovascular diseases (such as myocardial ischemia).

For research use only. We do not sell to patients.

Ischemin sodium Chemical Structure

Size	Price	Stock	Quantity
5 mg	USD 285	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 450	In-stock	Estimated Time of Arrival: December 31
50 mg		Get quote
100 mg		Get quote

or Bulk Inquiry

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

Biological Activity
Purity & Documentation
References
Customer Review

Description

In Vitro

Ischemin sodium (50, 100 µM; 24 h) inhibits p53 activation on DNA damaging stress in U2OS cells^[1].
Ischemin sodium (10 μM; 3 days) blocks apoptosis in cardiomyocytes by inhibiting caspase 3/7 activity^[1].
Ischemin sodium salt inhibits p53 cellular signaling pathways in U2OS cells^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[1]

Cell Line:	U2OS cells
Concentration:	50, 100 μM
Incubation Time:	24 h
Result:	Inhibited the Doxorubicin-induced increased levels of p53 protein, its Ser15-phosphorylated (p53S15p) and Lys382-acetylated (p53K382ac).

Apoptosis Analysis^[1]

Cell Line:	U2OS cells
Concentration:	10 μM
Incubation Time:	3 days
Result:	Functioned as a cellular protective agent againsted myocardial ischemic stress.

Molecular Weight

357.36

Formula

C₁₅H₁₆N₃NaO₄S

Appearance

Solid

Color

Light brown to brown

SMILES

CC1=CC(/N=N/C2=C(C=C(C(S(=O)(O[Na])=O)=C2)C)C)=C(C=C1O)N

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility

In Vitro:

H₂O : 36 mg/mL (100.74 mM; Need ultrasonic and warming)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.7983 mL	13.9915 mL	27.9830 mL
5 mM	0.5597 mL	2.7983 mL	5.5966 mL
10 mM	0.2798 mL	1.3991 mL	2.7983 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.32%

Select Batch:

Data Sheet (272 KB) SDS (252 KB)

COA (248 KB) LCMS (100 KB)

Handling Instructions (2659 KB)

References

[1]. Borah JC, et al. A small molecule binding to the coactivator CREB-binding protein blocks apoptosis in cardiomyocytes. Chem Biol. 2011 Apr 22;18(4):531-41. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O	1 mM	2.7983 mL	13.9915 mL	27.9830 mL	69.9575 mL
	5 mM	0.5597 mL	2.7983 mL	5.5966 mL	13.9915 mL
	10 mM	0.2798 mL	1.3991 mL	2.7983 mL	6.9957 mL
	15 mM	0.1866 mL	0.9328 mL	1.8655 mL	4.6638 mL
	20 mM	0.1399 mL	0.6996 mL	1.3991 mL	3.4979 mL
	25 mM	0.1119 mL	0.5597 mL	1.1193 mL	2.7983 mL
	30 mM	0.0933 mL	0.4664 mL	0.9328 mL	2.3319 mL
	40 mM	0.0700 mL	0.3498 mL	0.6996 mL	1.7489 mL
	50 mM	0.0560 mL	0.2798 mL	0.5597 mL	1.3991 mL
	60 mM	0.0466 mL	0.2332 mL	0.4664 mL	1.1660 mL
	80 mM	0.0350 mL	0.1749 mL	0.3498 mL	0.8745 mL
	100 mM	0.0280 mL	0.1399 mL	0.2798 mL	0.6996 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Ischemin sodium Related Classifications

Cardiovascular Disease

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

IscheminApoptosisMDM-2/p53Epigenetic Reader DomainU2OS293THEK293Tmyocardial ischemiap21apoptosiscardiomyocytesInhibitorinhibitorinhibit

Your Recently Viewed Products:

Product Name

Salutation

Applicant Name *

Email Address *

Phone Number *

Organization Name *

Department *

Requested quantity *

Country or Region *

Remarks