2. Natural Products
  3. Plants Flavonoids
  4. Leguminosae Flavones
  5. Genista ephedroides DC.
  6. Isokaempferide

Isokaempferide  (Synonyms: Kaempferol 3-methyl ether; Kaempferol 3-O-methyl ether)

Cat. No.: HY-N3476 Purity: 99.88%
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Isokaempferide is a flavonoid can be isolated from the aerial parts of Genista ephedroides.

For research use only. We do not sell to patients.

Isokaempferide Chemical Structure

Isokaempferide Chemical Structure

CAS No. : 1592-70-7

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1 mg USD 390 In-stock
5 mg USD 986 In-stock
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Based on 1 publication(s) in Google Scholar

Isokaempferide Related Antibodies

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Description

Isokaempferide is a flavonoid can be isolated from the aerial parts of Genista ephedroides[1].

Cellular Effect
Cell Line Type Value Description References
B16 IC50
75.8 μM
Compound: 12, isokaempferide, kaempferol-3-monomethylether
Cytotoxicity against mouse B16 cells assessed as cell viability after 48 hrs by MTT assay
Cytotoxicity against mouse B16 cells assessed as cell viability after 48 hrs by MTT assay
[PMID: 25659770]
HepG2 IC50
62.5 μM
Compound: 15
Cytotoxicity against human HepG2 cells assessed as growth inhibition after 72 hrs by neutral red assay
Cytotoxicity against human HepG2 cells assessed as growth inhibition after 72 hrs by neutral red assay
[PMID: 28318944]
KB IC50
64.8 μM
Compound: 15
Cytotoxicity against human KB cells assessed as growth inhibition after 72 hrs by neutral red assay
Cytotoxicity against human KB cells assessed as growth inhibition after 72 hrs by neutral red assay
[PMID: 28318944]
Lu1 IC50
60.1 μM
Compound: 15
Cytotoxicity against human Lu1 cells assessed as growth inhibition after 72 hrs by neutral red assay
Cytotoxicity against human Lu1 cells assessed as growth inhibition after 72 hrs by neutral red assay
[PMID: 28318944]
MCF7 IC50
55.4 μM
Compound: 15
Cytotoxicity against human MCF7 cells assessed as growth inhibition after 72 hrs by neutral red assay
Cytotoxicity against human MCF7 cells assessed as growth inhibition after 72 hrs by neutral red assay
[PMID: 28318944]
Molecular Weight

300.26

Formula

C16H12O6
CAS No.
Appearance

Solid

Color

Light yellow to yellow

SMILES

O=C1C2=C(O)C=C(O)C=C2OC(C3=CC=C(O)C=C3)=C1OC
Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 250 mg/mL (832.61 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.3304 mL 16.6522 mL 33.3045 mL
5 mM 0.6661 mL 3.3304 mL 6.6609 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.3304 mL 16.6522 mL 33.3045 mL 83.2612 mL
5 mM 0.6661 mL 3.3304 mL 6.6609 mL 16.6522 mL
10 mM 0.3330 mL 1.6652 mL 3.3304 mL 8.3261 mL
15 mM 0.2220 mL 1.1101 mL 2.2203 mL 5.5507 mL
20 mM 0.1665 mL 0.8326 mL 1.6652 mL 4.1631 mL
25 mM 0.1332 mL 0.6661 mL 1.3322 mL 3.3304 mL
30 mM 0.1110 mL 0.5551 mL 1.1101 mL 2.7754 mL
40 mM 0.0833 mL 0.4163 mL 0.8326 mL 2.0815 mL
50 mM 0.0666 mL 0.3330 mL 0.6661 mL 1.6652 mL
60 mM 0.0555 mL 0.2775 mL 0.5551 mL 1.3877 mL
80 mM 0.0416 mL 0.2082 mL 0.4163 mL 1.0408 mL
100 mM 0.0333 mL 0.1665 mL 0.3330 mL 0.8326 mL
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Isokaempferide Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Isokaempferide1592-70-7Kaempferol 3-methyl ether Kaempferol 3-O-methyl etherOthersflavonoidInhibitorinhibitorinhibit

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